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Q8FMD0 (DEF2_COREF) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Peptide deformylase 2
Short name=PDF 2
EC=3.5.1.88
Alternative name(s):
Polypeptide deformylase 2
Gene names
Name:def2
Ordered Locus Names:CE2577
OrganismCorynebacterium efficiens [Complete proteome] [HAMAP]
Taxonomic identifier152794 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length193 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163

Catalytic activity

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163

Cofactor

Binds 1 Fe2+ ion By similarity. HAMAP MF_00163

Sequence similarities

Belongs to the polypeptide deformylase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandIron
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtranslation

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functioniron ion binding

Inferred from electronic annotation. Source: InterPro

peptide deformylase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 193193Peptide deformylase 2 HAMAP MF_00163
PRO_0000082773

Sites

Active site1431 By similarity
Metal binding1001Iron By similarity
Metal binding1421Iron By similarity
Metal binding1461Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FMD0 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 8596E2B1D2337925

FASTA19321,168
        10         20         30         40         50         60 
MTVRPIVIHG DPVLHNPTRE VTEPISELQE LIADMYETME VANGVGLAAN QIGVSKRIFV 

        70         80         90        100        110        120 
FNCPDDEGTM HRGCFINPVL ETSEIPETMP ADDGSDEEGC LSVPGEGFPT GRADWAKVTG 

       130        140        150        160        170        180 
LNEDGEEWSM EGTGFLARCF QHEVGHLDGV VYTDTLIGRW KRLAKKTIKA NGWTEPGLTW 

       190 
TPGVDEDPFG HDV

« Hide

References

[1]"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000035 Genomic DNA. Translation: BAC19387.1.
RefSeqNP_739187.1. NC_004369.1.

3D structure databases

ProteinModelPortalQ8FMD0.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1034243.
GenomeReviewsGene locus CE2577 in contig BA000035_GR.
KEGGcef:CE2577.
NMPDRfig|196164.1.peg.2577.
PATRIC21491301. VBICorEff9312_2544.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG665227.
OMATWMPGED.
PhylomeDBQ8FMD0.
ProtClustDBPRK12846.

Enzyme and pathway databases

BioCycCEFF196164:CE2577-MONOMER.

Family and domain databases

HAMAPMF_00163. Pep_deformylase.
[Tree]
InterProIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
Gene3DG3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KOK01462.
PANTHERPTHR10458. Fmet_deformylase. 1 hit.
PfamPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFPIRSF004749. Pep_def. 1 hit.
PRINTSPR01576. PDEFORMYLASE.
SUPFAMSSF56420. Fmet_deformylase. 1 hit.
TIGRFAMsTIGR00079. Pept_deformyl. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDEF2_COREF
AccessionPrimary (citable) accession number: Q8FMD0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2003
Last modified: January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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