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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi207 – 214ATP 1By similarity8
Nucleotide bindingi608 – 615ATP 2By similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:CE2613
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001409 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001911151 – 852Chaperone protein ClpBAdd BLAST852

Proteomic databases

PRIDEiQ8FM94

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Protein-protein interaction databases

STRINGi196164.HMPREF0290_0256

Structurei

3D structure databases

ProteinModelPortaliQ8FM94
SMRiQ8FM94
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 144N-terminalBy similarityAdd BLAST144
Regioni160 – 342NBD1By similarityAdd BLAST183
Regioni343 – 548LinkerBy similarityAdd BLAST206
Regioni558 – 756NBD2By similarityAdd BLAST199
Regioni757 – 852C-terminalBy similarityAdd BLAST96

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili393 – 527By similarityAdd BLAST135

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG4105C2Z Bacteria
COG0542 LUCA
HOGENOMiHOG000218211
KOiK03695
OMAiHHKVRIK
OrthoDBiPOG091H019M

Family and domain databases

Gene3Di1.10.1780.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR017730 Chaperonin_ClpB
IPR019489 Clp_ATPase_C
IPR004176 Clp_N
IPR036628 Clp_N_dom_sf
IPR001270 ClpA/B
IPR018368 ClpA/B_CS1
IPR028299 ClpA/B_CS2
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF07724 AAA_2, 1 hit
PF02861 Clp_N, 2 hits
PF10431 ClpB_D2-small, 1 hit
PRINTSiPR00300 CLPPROTEASEA
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01086 ClpB_D2-small, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
SSF81923 SSF81923, 1 hit
TIGRFAMsiTIGR03346 chaperone_ClpB, 1 hit
PROSITEiView protein in PROSITE
PS00870 CLPAB_1, 1 hit
PS00871 CLPAB_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8FM94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSFNPTTKT SEAMQAALQQ ASANGNPDIR PAHLLVAILD QADGVAAPVL
60 70 80 90 100
TAAGVDPKTI LAEAQKLVDG YPKASGSNLA NPNFNRDALN ALTASQELAG
110 120 130 140 150
ELGDEYVSTE VLLAGIARGK SDAADLLKGK GATYDAIKAA FQSVRGSQKV
160 170 180 190 200
TSQDPEGQFQ ALEKYSTDLT KLAREGKIDP VIGRDQEIRR VVQVLSRRTK
210 220 230 240 250
NNPVLIGEPG VGKTAIVEGL ARRIVAGDVP ESLKGKTLIS LDLGSMVAGA
260 270 280 290 300
KYRGEFEERL KAVLDEIKGA NGEIVTFIDE LHTIVGAGAS GESAMDAGNM
310 320 330 340 350
IKPLLARGEL RLVGATTLNE YRKYIEKDTA LERRFQQVYV GEPSVEDTVG
360 370 380 390 400
ILRGLKERYE VHHGVRIQDS ALVAAAELSH RYITSRFLPD KAIDLVDEAA
410 420 430 440 450
SRLRMEIDSS PQEIDELERI VRRLEIEEVA LTKETDVASR ERLERLRSEL
460 470 480 490 500
ADEREKLSEL KARWQNEKAV IDDVRKFKEE LEALRSESDI AEREGDYGRV
510 520 530 540 550
AELRYGRIPE LEKKIAEAEE KIGGADNSML TEEVTPEVIA EVVSAWTGIP
560 570 580 590 600
AGKMMQGETE KLLNMERFLG KRVVGQHEAV TAVSDAVRRS RAGVADPNRP
610 620 630 640 650
TGSFLFLGPT GVGKTELAKA VSEFLFDDER AMVRIDMSEY SEKHSVARLV
660 670 680 690 700
GAPPGYVGYD QGGQLTEAVR RRPYTTVLFD EVEKAHPDVF DILLQVLDDG
710 720 730 740 750
RLTDGQGRTV DFRNTILILT SNLGAGGTRE QMMDAVKMAF KPEFINRLDD
760 770 780 790 800
IVVFDPLSQE QLASIVEIQI SQLAERLSDR RLTLRVSDAA KLWLAERGYD
810 820 830 840 850
PAYGARPLRR LIQQAIGDQL AKELLAGEIR DGDRVLVDVA DGGQYLAVSR

EH
Length:852
Mass (Da):93,445
Last modified:March 1, 2003 - v1
Checksum:i426359F92D8BAB0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA Translation: BAC19423.1
RefSeqiWP_011076009.1, NZ_GG700685.1

Genome annotation databases

EnsemblBacteriaiBAC19423; BAC19423; BAC19423
KEGGicef:CE2613

Similar proteinsi

Entry informationi

Entry nameiCLPB_COREF
AccessioniPrimary (citable) accession number: Q8FM94
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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