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Q8FM16

- PCKG_COREF

UniProt

Q8FM16 - PCKG_COREF

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821SubstrateUniRule annotation
Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
Metal bindingi230 – 2301ManganeseUniRule annotation
Binding sitei230 – 2301SubstrateUniRule annotation
Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
Binding sitei272 – 2721SubstrateUniRule annotation
Active sitei274 – 2741UniRule annotation
Metal bindingi297 – 2971ManganeseUniRule annotation
Binding sitei390 – 3901GTPUniRule annotation
Binding sitei421 – 4211GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi273 – 2786GTPUniRule annotation
Nucleotide bindingi516 – 5194GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pck
Ordered Locus Names:CE2691
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001409: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 612612Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103602Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi196164.CE2691.

Structurei

3D structure databases

ProteinModelPortaliQ8FM16.
SMRiQ8FM16. Positions 13-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni388 – 3903Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000191700.
KOiK01596.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8FM16 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTAAIPGLQ GEAPTNNQEL LNWIAEAVEL FQPEAVVFAD GSQEEWDRMA
60 70 80 90 100
EELVEAGTLI RLNEEKRPNS FLARSNPSDV ARVESRTFIC SENQEDAGPT
110 120 130 140 150
NNWAPPQAMK EEMTEVYRGS MKGRTMYVVP FCMGPITDPE PKLGVQLTDS
160 170 180 190 200
AYVVMSMRIM TRMGKDALDK IGENGSFVRC LHSVGAPLEE GQEDVAWPCN
210 220 230 240 250
DTKYITQFPE TKEIWSYGSG YGGNAILAKK CYALRIASVM AREEGWMAEH
260 270 280 290 300
MLILKLTNPE GQAYHIAAAF PSACGKTNLA MITPTIPGWK AEVVGDDIAW
310 320 330 340 350
LKFREDGHLY AVNPENGFFG VAPGTNYASN PIAMQTMEPG NTLFTNVALT
360 370 380 390 400
DDGDIWWEGM DGDAPEHLID WKGNDWTPES NQPAAHPNSR YCVAIEQCPT
410 420 430 440 450
AAPEFNDWKG VKVDAILFGG RRPDTVPLVT QTHDWEHGTM VGALLASGQT
460 470 480 490 500
AASAEAKVGT LRHDPMAMLP FMGYNAGEYL QNWIDMGNKG GDKMPSIFLV
510 520 530 540 550
NWFRRGEDGR FLWPGFGENS RVLKWVIDRI EGRVGAEETV VGYTARAEDL
560 570 580 590 600
DLEGLDTPIE DIREALTAPA EQWAADLEDN AEYLTFLGPK VPTEVHEQFE
610
ALKKRIQAAQ AS
Length:612
Mass (Da):67,642
Last modified:December 15, 2003 - v2
Checksum:iD9A27B4ED0521148
GO

Sequence cautioni

The sequence BAC19501.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000035 Genomic DNA. Translation: BAC19501.1. Different initiation.
RefSeqiNP_739301.1. NC_004369.1.

Genome annotation databases

EnsemblBacteriaiBAC19501; BAC19501; BAC19501.
GeneIDi1032110.
KEGGicef:CE2691.
PATRICi21491535. VBICorEff9312_2660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000035 Genomic DNA. Translation: BAC19501.1 . Different initiation.
RefSeqi NP_739301.1. NC_004369.1.

3D structure databases

ProteinModelPortali Q8FM16.
SMRi Q8FM16. Positions 13-606.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196164.CE2691.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC19501 ; BAC19501 ; BAC19501 .
GeneIDi 1032110.
KEGGi cef:CE2691.
PATRICi 21491535. VBICorEff9312_2660.

Phylogenomic databases

HOGENOMi HOG000191700.
KOi K01596.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
    Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
    Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Entry informationi

Entry nameiPCKG_COREF
AccessioniPrimary (citable) accession number: Q8FM16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: October 29, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3