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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821SubstrateUniRule annotation
Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
Metal bindingi230 – 2301ManganeseUniRule annotation
Binding sitei230 – 2301SubstrateUniRule annotation
Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
Binding sitei272 – 2721SubstrateUniRule annotation
Active sitei274 – 2741UniRule annotation
Metal bindingi297 – 2971ManganeseUniRule annotation
Binding sitei390 – 3901GTPUniRule annotation
Binding sitei421 – 4211GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi273 – 2786GTPUniRule annotation
Nucleotide bindingi516 – 5194GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pck
Ordered Locus Names:CE2691
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001409 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 612612Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103602Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi196164.CE2691.

Structurei

3D structure databases

ProteinModelPortaliQ8FM16.
SMRiQ8FM16. Positions 13-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni388 – 3903Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000191700.
KOiK01596.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8FM16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTAAIPGLQ GEAPTNNQEL LNWIAEAVEL FQPEAVVFAD GSQEEWDRMA
60 70 80 90 100
EELVEAGTLI RLNEEKRPNS FLARSNPSDV ARVESRTFIC SENQEDAGPT
110 120 130 140 150
NNWAPPQAMK EEMTEVYRGS MKGRTMYVVP FCMGPITDPE PKLGVQLTDS
160 170 180 190 200
AYVVMSMRIM TRMGKDALDK IGENGSFVRC LHSVGAPLEE GQEDVAWPCN
210 220 230 240 250
DTKYITQFPE TKEIWSYGSG YGGNAILAKK CYALRIASVM AREEGWMAEH
260 270 280 290 300
MLILKLTNPE GQAYHIAAAF PSACGKTNLA MITPTIPGWK AEVVGDDIAW
310 320 330 340 350
LKFREDGHLY AVNPENGFFG VAPGTNYASN PIAMQTMEPG NTLFTNVALT
360 370 380 390 400
DDGDIWWEGM DGDAPEHLID WKGNDWTPES NQPAAHPNSR YCVAIEQCPT
410 420 430 440 450
AAPEFNDWKG VKVDAILFGG RRPDTVPLVT QTHDWEHGTM VGALLASGQT
460 470 480 490 500
AASAEAKVGT LRHDPMAMLP FMGYNAGEYL QNWIDMGNKG GDKMPSIFLV
510 520 530 540 550
NWFRRGEDGR FLWPGFGENS RVLKWVIDRI EGRVGAEETV VGYTARAEDL
560 570 580 590 600
DLEGLDTPIE DIREALTAPA EQWAADLEDN AEYLTFLGPK VPTEVHEQFE
610
ALKKRIQAAQ AS
Length:612
Mass (Da):67,642
Last modified:December 15, 2003 - v2
Checksum:iD9A27B4ED0521148
GO

Sequence cautioni

The sequence BAC19501.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC19501.1. Different initiation.
RefSeqiNP_739301.1. NC_004369.1.
WP_006768941.1. NC_004369.1.
WP_035109279.1. NZ_GG700685.1.

Genome annotation databases

EnsemblBacteriaiBAC19501; BAC19501; BAC19501.
KEGGicef:CE2691.
PATRICi21491535. VBICorEff9312_2660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC19501.1. Different initiation.
RefSeqiNP_739301.1. NC_004369.1.
WP_006768941.1. NC_004369.1.
WP_035109279.1. NZ_GG700685.1.

3D structure databases

ProteinModelPortaliQ8FM16.
SMRiQ8FM16. Positions 13-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196164.CE2691.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC19501; BAC19501; BAC19501.
KEGGicef:CE2691.
PATRICi21491535. VBICorEff9312_2660.

Phylogenomic databases

HOGENOMiHOG000191700.
KOiK01596.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
    Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
    Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Entry informationi

Entry nameiPCKG_COREF
AccessioniPrimary (citable) accession number: Q8FM16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: May 27, 2015
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.