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Q8FM16 (PCKG_COREF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Gene names
Name:pckG
Synonyms:pck
Ordered Locus Names:CE2691
OrganismCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]
Taxonomic identifier196164 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length612 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Sequence caution

The sequence BAC19501.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 612612Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_0000103602

Regions

Nucleotide binding273 – 2786GTP By similarity
Nucleotide binding516 – 5194GTP By similarity
Region388 – 3903Substrate binding By similarity

Sites

Active site2741 By similarity
Metal binding2301Manganese By similarity
Metal binding2501Manganese; via tele nitrogen By similarity
Metal binding2971Manganese By similarity
Binding site821Substrate By similarity
Binding site2231Substrate; via amide nitrogen By similarity
Binding site2301Substrate By similarity
Binding site2721Substrate By similarity
Binding site3901GTP By similarity
Binding site4211GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FM16 [UniParc].

Last modified December 15, 2003. Version 2.
Checksum: D9A27B4ED0521148

FASTA61267,642
        10         20         30         40         50         60 
MTTAAIPGLQ GEAPTNNQEL LNWIAEAVEL FQPEAVVFAD GSQEEWDRMA EELVEAGTLI 

        70         80         90        100        110        120 
RLNEEKRPNS FLARSNPSDV ARVESRTFIC SENQEDAGPT NNWAPPQAMK EEMTEVYRGS 

       130        140        150        160        170        180 
MKGRTMYVVP FCMGPITDPE PKLGVQLTDS AYVVMSMRIM TRMGKDALDK IGENGSFVRC 

       190        200        210        220        230        240 
LHSVGAPLEE GQEDVAWPCN DTKYITQFPE TKEIWSYGSG YGGNAILAKK CYALRIASVM 

       250        260        270        280        290        300 
AREEGWMAEH MLILKLTNPE GQAYHIAAAF PSACGKTNLA MITPTIPGWK AEVVGDDIAW 

       310        320        330        340        350        360 
LKFREDGHLY AVNPENGFFG VAPGTNYASN PIAMQTMEPG NTLFTNVALT DDGDIWWEGM 

       370        380        390        400        410        420 
DGDAPEHLID WKGNDWTPES NQPAAHPNSR YCVAIEQCPT AAPEFNDWKG VKVDAILFGG 

       430        440        450        460        470        480 
RRPDTVPLVT QTHDWEHGTM VGALLASGQT AASAEAKVGT LRHDPMAMLP FMGYNAGEYL 

       490        500        510        520        530        540 
QNWIDMGNKG GDKMPSIFLV NWFRRGEDGR FLWPGFGENS RVLKWVIDRI EGRVGAEETV 

       550        560        570        580        590        600 
VGYTARAEDL DLEGLDTPIE DIREALTAPA EQWAADLEDN AEYLTFLGPK VPTEVHEQFE 

       610 
ALKKRIQAAQ AS 

« Hide

References

[1]"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000035 Genomic DNA. Translation: BAC19501.1. Different initiation.
RefSeqNP_739301.1. NC_004369.1.

3D structure databases

ProteinModelPortalQ8FM16.
SMRQ8FM16. Positions 13-606.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196164.CE2691.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC19501; BAC19501; BAC19501.
GeneID1032110.
KEGGcef:CE2691.
PATRIC21491535. VBICorEff9312_2660.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000191700.
KOK01596.
OrthoDBEOG6MPWQS.
ProtClustDBPRK04210.

Enzyme and pathway databases

UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_COREF
AccessionPrimary (citable) accession number: Q8FM16
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: April 16, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways