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Q8FM11 (TRMB_COREF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:CE2696
OrganismCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]
Taxonomic identifier196164 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171323

Regions

Region164 – 1696Interaction with RNA Potential
Region232 – 2354Substrate binding By similarity

Sites

Active site1581 By similarity
Binding site831S-adenosyl-L-methionine By similarity
Binding site1081S-adenosyl-L-methionine By similarity
Binding site1351S-adenosyl-L-methionine By similarity
Binding site1581S-adenosyl-L-methionine By similarity
Binding site1621Substrate By similarity
Binding site1941Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FM11 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: DCE8743C7364C5A3

FASTA25428,580
        10         20         30         40         50         60 
MSISDNSREE LGELPAGRPL QSEFNDDLDY PRLGSVTFRR GTLTENQQTM WNEKWPELGR 

        70         80         90        100        110        120 
VLEDELIDID AWFGRTGAKT IVEIGSGTGT STAAMAPLEA DTNIIAVELY KPGLAKLMGA 

       130        140        150        160        170        180 
VVRGGIDNIR MVRGDGIEVL NRMFADGSLD GVRIFFPDPW PKARHNKRRI IQSGPLNLIA 

       190        200        210        220        230        240 
KKLKPGGVLH VATDHADYAE WINELVEVEP LLEYKGWPWP ECPQLTDRQV ITKFEGKGLD 

       250 
KDHTINEYLW QKKQ 

« Hide

References

[1]"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000035 Genomic DNA. Translation: BAC19506.1.
RefSeqNP_739306.1. NC_004369.1.

3D structure databases

ProteinModelPortalQ8FM11.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196164.CE2696.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC19506; BAC19506; BAC19506.
GeneID1033792.
KEGGcef:CE2696.
PATRIC21491543. VBICorEff9312_2664.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000073968.
KOK03439.
OMALDKDHTI.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_COREF
AccessionPrimary (citable) accession number: Q8FM11
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways