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Protein

Glycerol kinase

Gene

glpK

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 171SubstrateUniRule annotation
Binding sitei21 – 211ATPUniRule annotation
Binding sitei139 – 1391SubstrateUniRule annotation
Binding sitei278 – 2781ATPUniRule annotation
Binding sitei322 – 3221ATP; via carbonyl oxygenUniRule annotation
Binding sitei326 – 3261ATP; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 193ATPUniRule annotation
Nucleotide bindingi423 – 4275ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCEFF196164:GJW8-2777-MONOMER.
UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:CE2721
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001409 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 508508Glycerol kinasePRO_0000059447Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi196164.HMPREF0290_0144.

Structurei

3D structure databases

ProteinModelPortaliQ8FLY8.
SMRiQ8FLY8. Positions 8-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 882Substrate bindingUniRule annotation
Regioni256 – 2572Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JQ0. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
KOiK00864.
OrthoDBiEOG6RZB46.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8FLY8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRASKANAYV AAIDQGTTST RCIIIDDTGA VVSVAAKEHE QILPQQGWVE
60 70 80 90 100
HDPVEIWDNV RAVVSQAMVA IDITPYEISS VGVTNQRETT VIWDPATGEP
110 120 130 140 150
VYNAIVWQDT RTNEICRELA GEEGQQKWLD RTGLLINSYP AGPKIKWILD
160 170 180 190 200
NVEGVRERAE KGELYFGTMD TWLLWNLTGG IRGDDGEEAL HVTDVTNASR
210 220 230 240 250
TLLMDIHTQQ WDPELCEALD IPMSLLPEIK PSIGDYRSVR HRGILADVPI
260 270 280 290 300
TGVLGDQQAA LFGQGGFKEG DTKNTYGTGL FLVMNTGPEL KISEHGLLST
310 320 330 340 350
IAYQIEGQDP VYALEGSVSM GGSLVQWLRD SLQMIPTAPA IEEFARQVPD
360 370 380 390 400
NGGVHIVPAF SGLFAPRWHP EARGVITGLT RFANRNHICR AVLEATAFQT
410 420 430 440 450
REVVDAMARD AGKELESLRV DGAMVQNELL MQMQADILGI DVIRPGDIET
460 470 480 490 500
TALGTAFAAG LGSGFFKDFD QIIELISIRQ TWTPEMTEAE RERAYDAWNE

AVTHAYPR
Length:508
Mass (Da):55,972
Last modified:April 26, 2004 - v2
Checksum:i608835CF8BB46632
GO

Sequence cautioni

The sequence BAC19531.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC19531.1. Different initiation.
RefSeqiWP_035109268.1. NZ_GG700685.1.

Genome annotation databases

EnsemblBacteriaiBAC19531; BAC19531; BAC19531.
KEGGicef:CE2721.
PATRICi21491593. VBICorEff9312_2689.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC19531.1. Different initiation.
RefSeqiWP_035109268.1. NZ_GG700685.1.

3D structure databases

ProteinModelPortaliQ8FLY8.
SMRiQ8FLY8. Positions 8-504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196164.HMPREF0290_0144.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC19531; BAC19531; BAC19531.
KEGGicef:CE2721.
PATRICi21491593. VBICorEff9312_2689.

Phylogenomic databases

eggNOGiENOG4108JQ0. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
KOiK00864.
OrthoDBiEOG6RZB46.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.
BioCyciCEFF196164:GJW8-2777-MONOMER.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
    Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
    Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Entry informationi

Entry nameiGLPK_COREF
AccessioniPrimary (citable) accession number: Q8FLY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: December 9, 2015
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.