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Q8FLM0

- SYL_COREF

UniProt

Q8FLM0 - SYL_COREF

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei730 – 7301ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:CE2848
    OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
    Taxonomic identifieri196164 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000001409: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 957957Leucine--tRNA ligasePRO_0000152006Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi196164.CE2848.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8FLM0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi70 – 8112"HIGH" regionAdd
    BLAST
    Motifi727 – 7315"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000200748.
    KOiK01869.
    OMAiYARYHRM.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 4 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8FLM0-1 [UniParc]FASTAAdd to Basket

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    MTNPSEGTSS SAQSLAYRYT PELANAIERE WQNYWTDKGT FNAPNPVGDL    50
    APEDGRELPG DKLFVQDMFP YPSGAGLHVG HPLGYIATDV FARYNRMLGK 100
    NVLHTLGYDA FGLPAEQYAI QTGTHPRTTT MANIENMKRQ LGALGLGHDP 150
    RRAVASTDPE FYKWTQWIFL QIFNSWFDEE QQKARPISEL IPLLESGEIP 200
    TRDGADYNGL DRVAKQKAID EFRLVYRSNS TVNWCPGLGT VLANEEVTAD 250
    GRSERGNFPV FRKNLSQWMM RITAYSDRLI DDLELLDWPE KVKSMQRNWI 300
    GRSRGAEVDF TAEGETITVF TTRPDTLFGA TYMVLAPEHE LVDVLVSRGT 350
    GSYDGVDPRW TNGQATPAEA VAAYRASIAA KSDLERQENK DKTGVFLGVH 400
    ATNPVNGEQI PVFIADYVLT GYGTGAIMAV PAHDDRDYEF ATVFGLPIVE 450
    VVAGGNIAEA AYTASGESIN SANDQGLDIN GLAMVDAVAR TIEWLEEKQL 500
    GRGTIQYKLR DWLFARQRYW GEPFPVVYDE DGVAYALPES MLPVELPEVE 550
    DYKPVSFDPE DADSEPSPPL AKAREWVEVE LDLGDGVKKY TRDTNVMPQW 600
    AGSSWYQLRY IDPTNDDQFC NLENEAYWTG PRPDVHGPND PGGVDLYVGG 650
    VEHAVLHLLY SRFWHKVLYD LGYVTSKEPY RRLYNQGYIQ AFAYTDSRGV 700
    YVPAEEVEEK DGKFFYQGEE VTQEYGKMGK SLKNAVAPDD ICNNYGADTL 750
    RVYEMSMGPL DTSRPWATKD VVGAQRFLQR LWRLIVDENT GEVLTRDEAL 800
    TDEDNKHLHR TIAGVRDDYA NLRVNTVVAK LIEYVNYLTK TYPDTIPTGA 850
    VLPLVVMVSP VAPHIAEELW KKLGHTDTVT YEPFPTFEEK WLTDDEVELP 900
    VQINGKVRSR ITVAADASQE QIIEVALADE KIALQIEGKN LIKQIVIPGR 950
    MVNLVVK 957
    Length:957
    Mass (Da):107,399
    Last modified:March 1, 2003 - v1
    Checksum:iA6FFBF26AAFBC1FF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000035 Genomic DNA. Translation: BAC19658.1.
    RefSeqiNP_739458.1. NC_004369.1.

    Genome annotation databases

    EnsemblBacteriaiBAC19658; BAC19658; BAC19658.
    GeneIDi1033530.
    KEGGicef:CE2848.
    PATRICi21491841. VBICorEff9312_2807.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000035 Genomic DNA. Translation: BAC19658.1 .
    RefSeqi NP_739458.1. NC_004369.1.

    3D structure databases

    ProteinModelPortali Q8FLM0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196164.CE2848.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC19658 ; BAC19658 ; BAC19658 .
    GeneIDi 1033530.
    KEGGi cef:CE2848.
    PATRICi 21491841. VBICorEff9312_2807.

    Phylogenomic databases

    HOGENOMi HOG000200748.
    KOi K01869.
    OMAi YARYHRM.
    OrthoDBi EOG63Z74X.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 4 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
      Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
      Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

    Entry informationi

    Entry nameiSYL_COREF
    AccessioniPrimary (citable) accession number: Q8FLM0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 25, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3