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Q8FLM0 (SYL_COREF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CE2848
OrganismCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]
Taxonomic identifier196164 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length957 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 957957Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152006

Regions

Motif70 – 8112"HIGH" region HAMAP-Rule MF_00049
Motif727 – 7315"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7301ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FLM0 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: A6FFBF26AAFBC1FF

FASTA957107,399
        10         20         30         40         50         60 
MTNPSEGTSS SAQSLAYRYT PELANAIERE WQNYWTDKGT FNAPNPVGDL APEDGRELPG 

        70         80         90        100        110        120 
DKLFVQDMFP YPSGAGLHVG HPLGYIATDV FARYNRMLGK NVLHTLGYDA FGLPAEQYAI 

       130        140        150        160        170        180 
QTGTHPRTTT MANIENMKRQ LGALGLGHDP RRAVASTDPE FYKWTQWIFL QIFNSWFDEE 

       190        200        210        220        230        240 
QQKARPISEL IPLLESGEIP TRDGADYNGL DRVAKQKAID EFRLVYRSNS TVNWCPGLGT 

       250        260        270        280        290        300 
VLANEEVTAD GRSERGNFPV FRKNLSQWMM RITAYSDRLI DDLELLDWPE KVKSMQRNWI 

       310        320        330        340        350        360 
GRSRGAEVDF TAEGETITVF TTRPDTLFGA TYMVLAPEHE LVDVLVSRGT GSYDGVDPRW 

       370        380        390        400        410        420 
TNGQATPAEA VAAYRASIAA KSDLERQENK DKTGVFLGVH ATNPVNGEQI PVFIADYVLT 

       430        440        450        460        470        480 
GYGTGAIMAV PAHDDRDYEF ATVFGLPIVE VVAGGNIAEA AYTASGESIN SANDQGLDIN 

       490        500        510        520        530        540 
GLAMVDAVAR TIEWLEEKQL GRGTIQYKLR DWLFARQRYW GEPFPVVYDE DGVAYALPES 

       550        560        570        580        590        600 
MLPVELPEVE DYKPVSFDPE DADSEPSPPL AKAREWVEVE LDLGDGVKKY TRDTNVMPQW 

       610        620        630        640        650        660 
AGSSWYQLRY IDPTNDDQFC NLENEAYWTG PRPDVHGPND PGGVDLYVGG VEHAVLHLLY 

       670        680        690        700        710        720 
SRFWHKVLYD LGYVTSKEPY RRLYNQGYIQ AFAYTDSRGV YVPAEEVEEK DGKFFYQGEE 

       730        740        750        760        770        780 
VTQEYGKMGK SLKNAVAPDD ICNNYGADTL RVYEMSMGPL DTSRPWATKD VVGAQRFLQR 

       790        800        810        820        830        840 
LWRLIVDENT GEVLTRDEAL TDEDNKHLHR TIAGVRDDYA NLRVNTVVAK LIEYVNYLTK 

       850        860        870        880        890        900 
TYPDTIPTGA VLPLVVMVSP VAPHIAEELW KKLGHTDTVT YEPFPTFEEK WLTDDEVELP 

       910        920        930        940        950 
VQINGKVRSR ITVAADASQE QIIEVALADE KIALQIEGKN LIKQIVIPGR MVNLVVK 

« Hide

References

[1]"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000035 Genomic DNA. Translation: BAC19658.1.
RefSeqNP_739458.1. NC_004369.1.

3D structure databases

ProteinModelPortalQ8FLM0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196164.CE2848.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC19658; BAC19658; BAC19658.
GeneID1033530.
KEGGcef:CE2848.
PATRIC21491841. VBICorEff9312_2807.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_COREF
AccessionPrimary (citable) accession number: Q8FLM0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries