Reviewed, UniProtKB/Swiss-Prot Q8FKI9 (BETA_ECOL6)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	c0431

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Choline dehydrogenase HAMAP MF_00750		PRO_0000205588
Regions
	Nucleotide binding	4 – 33	30	FAD Probable
Sites
	Active site	473	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FKI9-1 [UniParc]. Last modified February 16, 2004. Version 2. Checksum: 7958C0BD6D20BFC1	FASTA	556	61,899
	10 20 30 40 50 60 MQFDYIIIGA GSAGNVLATR LTEDPNTTVL LLEAGGPDYR FDFRTQMPAA LAFPLQGKRY 70 80 90 100 110 120 NWAYETEPEP FMNNRRMECG RGKGLGGSSL INGMCYIRGN ALDLDNWAQE PSLENWSYLD 130 140 150 160 170 180 CLPYYRKAET RDVGENDYHG GDGPVSVTTS KPGVNPLFEA MIEAGMQAGY PRTDDLNGYQ 190 200 210 220 230 240 QEGFGPMDRT VTPHGRRAST ARGYLDQAKS RPNLTIRTHA MTDHIIFDGK RAVGVEWLEG 250 260 270 280 290 300 DSTIPTRAAA NKEVLLCAGA IASPQILQRS GVGNAELLAE FDIPLVHELP GVGENLQDHL 310 320 330 340 350 360 EMYLQYECKE PVSLYPALQW WNQPRIGAEW LFGGTGVGAS NHFEAGGFIR SREEFAWPNI 370 380 390 400 410 420 QYHFLPVAIN YNGSNAVKEH GFQCHVGSMR SPSRGHVRIK SRDPHQHPGI LFNYMSHEQD 430 440 450 460 470 480 WQEFRDAIRI TREIMHQPAL DQYRGREISP GVECQTDEQL DEFVRNHAET AFHPCGTCKM 490 500 510 520 530 540 GYDEMAVVDG EGRVHGLEGL RVVDASIMPQ IITGNLNATT IMIGEKIADM IRGKEALPRS 550 TAGYFVANGM PVRAKK

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014075 Genomic DNA. Translation: AAN78912.1. Different initiation.
RefSeq	NP_752368.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1036260.
GenomeReviews	Gene locus c0431 in contig AE014075_GR.
KEGG	ecc:c0431.
NMPDR	fig|199310.1.peg.412.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8FKI9.
OMA	Q8FKI9. PNLQYHF.
Enzyme and pathway databases
BRENDA	1.1.99.1. 292881.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_ECOL6

Accession

Primary (citable) accession number: Q8FKI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	June 16, 2009
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)