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Reviewed, UniProtKB/Swiss-Prot Q8FKG6 (BGAL_ECOL6)

Last modified January 19, 2010. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Beta-galactosidase
      Short name=Beta-gal
    EC=3.2.1.23
Alternative name(s):
    Lactase
Gene names
Name: lacZ
Ordered Locus Names: c0459
OrganismEscherichia coli O6 [Complete proteome] [HAMAP]
Taxonomic identifier217992 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length1024 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP MF_01687

Subunit structure

Homotetramer By similarity. HAMAP MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10241024Beta-galactosidase HAMAP MF_01687
PRO_0000366996

Regions

Region538 – 5414Substrate binding By similarity

Sites

Active site4621Proton donor By similarity
Active site5381Nucleophile By similarity
Metal binding2021Sodium By similarity
Metal binding4171Magnesium 1 By similarity
Metal binding4191Magnesium 1 By similarity
Metal binding4621Magnesium 1 By similarity
Metal binding5981Magnesium 2 By similarity
Metal binding6021Sodium; via carbonyl oxygen By similarity
Metal binding6051Sodium By similarity
Binding site1031Substrate By similarity
Binding site2021Substrate By similarity
Binding site4621Substrate By similarity
Binding site6051Substrate By similarity
Binding site10001Substrate By similarity
Site3581Transition state stabilizer By similarity
Site3921Transition state stabilizer By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q8FKG6-1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: FD2A3EDED90D07F8

FASTA1,024116,495
        10         20         30         40         50         60 
MTMITDSLAV VLQRRDWENP GVTQLNRLAA HPPFASWRNS EEARTDRPSQ QLRSLNGEWR 

        70         80         90        100        110        120 
FAWFPAPEAV PESWLECDLP DADTVVVPSN WQMHGYDAPI YTNVTYPITV NPPFVPAENP 

       130        140        150        160        170        180 
TGCYSLTFNI DESWLQEGQT RIIFDGVNSA FHLWCNGRWV GYGQDSRLPS EFDLSAFLRA 

       190        200        210        220        230        240 
GENRLAVMVL RWSDGSYLED QDMWRMSGIF RDVSLLHKPT TQISDFQVTT LFNDDFSRAV 

       250        260        270        280        290        300 
LEAEVQMYGE LRDELRVTVS LWQGETQVAS GTAPFGGEII DERGGYADRV TLRLNVENPE 

       310        320        330        340        350        360 
LWSAEIPNLY RAVVELHTAD GTLIEAEACD VGFREVRIEN GLLLLNGKPL LIRGVNRHEH 

       370        380        390        400        410        420 
HPLHGQVMDE QTMVQDILLM KQNNFNAVRC SHYPNHPLWY TLCDRYGLYV VDEANIETHG 

       430        440        450        460        470        480 
MVPMNRLTDD PRWLPAMSER VTRMVQRDRN HPSVIIWSLG NESGHGANHD ALYRWIKSVD 

       490        500        510        520        530        540 
PSRPVQYEGG GADTTATDII CPMYARVDED QPFPAVPKWS IKKWLSLPGE MRPLILCEYA 

       550        560        570        580        590        600 
HAMGNSLGGF AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD ENGNPWSAYG GDFGDTPNDR 

       610        620        630        640        650        660 
QFCMNGLVFA DRTPHPALTE AKHQQQYFQF RLSGRTIEVT SEYLFRHSDN EFLHWMVALD 

       670        680        690        700        710        720 
GKPLASGEVP LDVGPQGKQL IELPELPQPE SAGQLWLTVR VVQPNATAWS EAGHISAWQQ 

       730        740        750        760        770        780 
WRLAENLSVT LPSASHAIPQ LTTSGTDFCI ELGNKRWQFN RQSGFLSQMW IGDEKQLLTP 

       790        800        810        820        830        840 
LRDQFTRAPL DNDIGVSEAT RIDPNAWVER WKAAGHYQAE AALLQCTADT LADAVLITTA 

       850        860        870        880        890        900 
HAWQHQGKTL FISRKTYRID GHGEMVINVD VAVASDTPHP ARIGLTCQLA QVSERVNWLG 

       910        920        930        940        950        960 
LGPQENYPDR LTAACFDRWD LPLSDMYTPY VFPSENGLRC GTRELNYGPH QWRGDFQFNI 

       970        980        990       1000       1010       1020 
SRYSQQQLME TSHRHLLHAE EGTWLNIDGF HMGIGGDDSW SPSVSAEFQL SAGRYHYQLV 


WCQK

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References

[1]"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli."
Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R.
Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE014075 Genomic DNA. Translation: AAN78938.1.
RefSeqNP_752394.1.

3D structure databases

HSSPHSSP built from PDB template 1BGL based on UniProtKB P00722.
SMRQ8FKG6. Positions 4-1024.
ModBaseSearch...

Genome annotation databases

GeneID1036228.
GenomeReviewsGene locus c0459 in contig AE014075_GR.
KEGGecc:c0459.
NMPDRfig|199310.1.peg.438.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG670643.
OMAQMCGELR.

Family and domain databases

HAMAPMF_01687. Beta_gal.
[Tree]
InterProIPR008979. Galactose-bd-like.
IPR011013. Glyco_hydro-type_carb-bd.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006104. Glyco_hydro_2_carb-bd.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006103. Glyco_hydro_2_TIM.
IPR004199. Glyco_hydro_42_D5.
IPR017853. Glyco_hydro_catalytic_core.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits.
G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameBGAL_ECOL6
AccessionPrimary (citable) accession number: Q8FKG6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 1, 2003
Last modified: January 19, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents