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Q8FKG6

- BGAL_ECOL6

UniProt

Q8FKG6 - BGAL_ECOL6

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Protein
Beta-galactosidase
Gene
lacZ, c0459
Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031Substrate By similarity
Metal bindingi202 – 2021Sodium By similarity
Binding sitei202 – 2021Substrate By similarity
Sitei358 – 3581Transition state stabilizer By similarity
Sitei392 – 3921Transition state stabilizer By similarity
Metal bindingi417 – 4171Magnesium 1 By similarity
Metal bindingi419 – 4191Magnesium 1 By similarity
Active sitei462 – 4621Proton donor By similarity
Metal bindingi462 – 4621Magnesium 1 By similarity
Binding sitei462 – 4621Substrate By similarity
Active sitei538 – 5381Nucleophile By similarity
Metal bindingi598 – 5981Magnesium 2 By similarity
Metal bindingi602 – 6021Sodium; via carbonyl oxygen By similarity
Metal bindingi605 – 6051Sodium By similarity
Binding sitei605 – 6051Substrate By similarity
Binding sitei1000 – 10001Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciECOL199310:C0459-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:c0459
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001410: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10241024Beta-galactosidaseUniRule annotation
PRO_0000366996Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi199310.c0459.

Structurei

3D structure databases

ProteinModelPortaliQ8FKG6.
SMRiQ8FKG6. Positions 14-1024.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni538 – 5414Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000252443.
KOiK01190.
OMAiDPKKPYI.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8FKG6-1 [UniParc]FASTAAdd to Basket

« Hide

MTMITDSLAV VLQRRDWENP GVTQLNRLAA HPPFASWRNS EEARTDRPSQ     50
QLRSLNGEWR FAWFPAPEAV PESWLECDLP DADTVVVPSN WQMHGYDAPI 100
YTNVTYPITV NPPFVPAENP TGCYSLTFNI DESWLQEGQT RIIFDGVNSA 150
FHLWCNGRWV GYGQDSRLPS EFDLSAFLRA GENRLAVMVL RWSDGSYLED 200
QDMWRMSGIF RDVSLLHKPT TQISDFQVTT LFNDDFSRAV LEAEVQMYGE 250
LRDELRVTVS LWQGETQVAS GTAPFGGEII DERGGYADRV TLRLNVENPE 300
LWSAEIPNLY RAVVELHTAD GTLIEAEACD VGFREVRIEN GLLLLNGKPL 350
LIRGVNRHEH HPLHGQVMDE QTMVQDILLM KQNNFNAVRC SHYPNHPLWY 400
TLCDRYGLYV VDEANIETHG MVPMNRLTDD PRWLPAMSER VTRMVQRDRN 450
HPSVIIWSLG NESGHGANHD ALYRWIKSVD PSRPVQYEGG GADTTATDII 500
CPMYARVDED QPFPAVPKWS IKKWLSLPGE MRPLILCEYA HAMGNSLGGF 550
AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD ENGNPWSAYG GDFGDTPNDR 600
QFCMNGLVFA DRTPHPALTE AKHQQQYFQF RLSGRTIEVT SEYLFRHSDN 650
EFLHWMVALD GKPLASGEVP LDVGPQGKQL IELPELPQPE SAGQLWLTVR 700
VVQPNATAWS EAGHISAWQQ WRLAENLSVT LPSASHAIPQ LTTSGTDFCI 750
ELGNKRWQFN RQSGFLSQMW IGDEKQLLTP LRDQFTRAPL DNDIGVSEAT 800
RIDPNAWVER WKAAGHYQAE AALLQCTADT LADAVLITTA HAWQHQGKTL 850
FISRKTYRID GHGEMVINVD VAVASDTPHP ARIGLTCQLA QVSERVNWLG 900
LGPQENYPDR LTAACFDRWD LPLSDMYTPY VFPSENGLRC GTRELNYGPH 950
QWRGDFQFNI SRYSQQQLME TSHRHLLHAE EGTWLNIDGF HMGIGGDDSW 1000
SPSVSAEFQL SAGRYHYQLV WCQK 1024
Length:1,024
Mass (Da):116,495
Last modified:March 1, 2003 - v1
Checksum:iFD2A3EDED90D07F8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014075 Genomic DNA. Translation: AAN78938.1.
RefSeqiNP_752394.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN78938; AAN78938; c0459.
GeneIDi1036228.
KEGGiecc:c0459.
PATRICi18278972. VBIEscCol75197_0431.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014075 Genomic DNA. Translation: AAN78938.1 .
RefSeqi NP_752394.1. NC_004431.1.

3D structure databases

ProteinModelPortali Q8FKG6.
SMRi Q8FKG6. Positions 14-1024.
ModBasei Search...

Protein-protein interaction databases

STRINGi 199310.c0459.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN78938 ; AAN78938 ; c0459 .
GeneIDi 1036228.
KEGGi ecc:c0459.
PATRICi 18278972. VBIEscCol75197_0431.

Phylogenomic databases

HOGENOMi HOG000252443.
KOi K01190.
OMAi DPKKPYI.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci ECOL199310:C0459-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFT073 / ATCC 700928 / UPEC.

Entry informationi

Entry nameiBGAL_ECOL6
AccessioniPrimary (citable) accession number: Q8FKG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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