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Protein

Glutamine--tRNA ligase

Gene

glnS

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei67L-glutamineUniRule annotation1
Binding sitei212L-glutamineUniRule annotation1
Binding sitei231ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi35 – 37ATPUniRule annotation3
Nucleotide bindingi41 – 47ATPUniRule annotation7
Nucleotide bindingi261 – 262ATPUniRule annotation2
Nucleotide bindingi269 – 271ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL199310:C0766-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligaseUniRule annotation (EC:6.1.1.18UniRule annotation)
Alternative name(s):
Glutaminyl-tRNA synthetaseUniRule annotation
Short name:
GlnRSUniRule annotation
Gene namesi
Name:glnSUniRule annotation
Ordered Locus Names:c0766
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001410 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001958342 – 554Glutamine--tRNA ligaseAdd BLAST553

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi199310.c0766

Structurei

3D structure databases

ProteinModelPortaliQ8FJW4
SMRiQ8FJW4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni317 – 324Interaction with tRNAUniRule annotation8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi34 – 44"HIGH" regionUniRule annotationAdd BLAST11
Motifi268 – 272"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CX6 Bacteria
COG0008 LUCA
HOGENOMiHOG000259232
KOiK01886
OMAiVTHSICT

Family and domain databases

Gene3Di1.10.1160.101 hit
2.40.240.102 hits
3.40.50.6202 hits
HAMAPiMF_00126 Gln_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR004514 Gln-tRNA-synth
IPR022861 Gln_tRNA_ligase_bac
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059 Glu/Gln-tRNA-synth_Ib_codon-bd
IPR020061 Glu_tRNA_lig_a-bdl
IPR020056 Rbsml_L25/Gln-tRNA_synth_N
IPR011035 Ribosomal_L25/Gln-tRNA_synth
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PF03950 tRNA-synt_1c_C, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF50715 SSF50715, 1 hit
TIGRFAMsiTIGR00440 glnS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8FJW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEAEARPTN FIRQIIDEDL ASGKHTTVHT RFPPEPNGYL HIGHAKSICL
60 70 80 90 100
NFGIAQDYKG QCNLRFDDTN PVKEDIEYVD SIKNDVEWLG FHWSGNVRYS
110 120 130 140 150
SDYFDQLHAY AIELINKGLA YVDELTPEQI REYRGTLTQP GKNSPYRDRS
160 170 180 190 200
VEENLALFEK MRTGGFEEGK ACLRAKIDMA SPFIVMRDPV LYRIKFAEHH
210 220 230 240 250
QTGNKWCIYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL YDWVLDNITI
260 270 280 290 300
PVHPRQYEFS RLNLEYTVMS KRKLNLLVTD KHVEGWDDPR MPTISGLRRR
310 320 330 340 350
GYTAASIREF CKRIGVTKQD NTIEMASLES CIREDLNENA PRAMAVIDPV
360 370 380 390 400
KLVIENYQGE GEMVTMPNHP NKPEMGSRQV PFSGEIWIDR ADFREEANKQ
410 420 430 440 450
YKRLVLGKEV RLRNAYVIKA ERVEKDAEGN ITTIFCTYDA DTLSKDPADG
460 470 480 490 500
RKVKGVIHWV SAAHALPVEI RLYDRLFSVP NPGAADDFLS VINPESLVIK
510 520 530 540 550
QGFAEPSLKD AVAGKAFQFE REGYFCLDSR HSTAEKPVFN RTVGLRDTWA

KVGE
Length:554
Mass (Da):63,494
Last modified:January 23, 2007 - v3
Checksum:iFE93254893E811F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA Translation: AAN79239.1
RefSeqiWP_001287134.1, NC_004431.1

Genome annotation databases

EnsemblBacteriaiAAN79239; AAN79239; c0766
KEGGiecc:c0766

Similar proteinsi

Entry informationi

Entry nameiSYQ_ECOL6
AccessioniPrimary (citable) accession number: Q8FJW4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: January 23, 2007
Last modified: February 28, 2018
This is version 101 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome