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Protein

Galactokinase

Gene

galK

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P).UniRule annotation

Catalytic activityi

ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei28 – 281Transition state stabilizerUniRule annotation
Metal bindingi130 – 1301MagnesiumUniRule annotation
Metal bindingi162 – 1621MagnesiumUniRule annotation
Active sitei174 – 1741Proton acceptorUniRule annotation
Binding sitei223 – 2231SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi124 – 1307ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. galactokinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. galactose metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL199310:C0833-MONOMER.
UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
GalactokinaseUniRule annotation (EC:2.7.1.6UniRule annotation)
Alternative name(s):
Galactose kinaseUniRule annotation
Gene namesi
Name:galKUniRule annotation
Ordered Locus Names:c0833
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001410: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 382381GalactokinasePRO_0000184609Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi199310.c0833.

Structurei

3D structure databases

ProteinModelPortaliQ8FJS1.
SMRiQ8FJS1. Positions 3-381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 374Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the GHMP kinase family. GalK subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000241100.
KOiK00849.
OMAiTFYVCKA.
OrthoDBiEOG6DG2SH.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00246. Galactokinase.
InterProiIPR000705. Galactokinase.
IPR022963. Galactokinase_bac.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8FJS1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLKEKTQSL FANAFGYPAT HTIQAPGRVN LIGEHTDYND GFVLPCAIDY
60 70 80 90 100
QTVISCAPRD DRKVRVMAAD YENQLDEFSL DAPIVAHENY QWANYVRGVV
110 120 130 140 150
KHLQLRNNSF GGVDMVISGN VPQGAGLSSS ASLEVAVGTV LQQLYHLPLD
160 170 180 190 200
GAQIALNGQE AENQFVGCNC GIMDQLISAL GKKDHALLID CRSLGTKAVS
210 220 230 240 250
MPKGVAVVII NSNFKRTLVG SEYNTRREQC ETGARFFQQP ALRDVTIEEF
260 270 280 290 300
NAVAHELDPI VAKRVRHILT ENARTVEAAS ALEQGDLKRM GELMAESHAS
310 320 330 340 350
MRDDFEITVP QIDTLVEIVK AVIGDKGGVR MTGGGFGGCI VALFPEELVP
360 370 380
AVQQAVAEQY EAKTGIKETF YVCKPSQGAG QC
Length:382
Mass (Da):41,476
Last modified:January 23, 2007 - v3
Checksum:iBE4B6CE6FC48F9A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN79306.1.
RefSeqiNP_752763.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN79306; AAN79306; c0833.
GeneIDi1038288.
KEGGiecc:c0833.
PATRICi18279725. VBIEscCol75197_0792.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN79306.1.
RefSeqiNP_752763.1. NC_004431.1.

3D structure databases

ProteinModelPortaliQ8FJS1.
SMRiQ8FJS1. Positions 3-381.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi199310.c0833.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN79306; AAN79306; c0833.
GeneIDi1038288.
KEGGiecc:c0833.
PATRICi18279725. VBIEscCol75197_0792.

Phylogenomic databases

HOGENOMiHOG000241100.
KOiK00849.
OMAiTFYVCKA.
OrthoDBiEOG6DG2SH.

Enzyme and pathway databases

UniPathwayiUPA00214.
BioCyciECOL199310:C0833-MONOMER.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00246. Galactokinase.
InterProiIPR000705. Galactokinase.
IPR022963. Galactokinase_bac.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFT073 / ATCC 700928 / UPEC.

Entry informationi

Entry nameiGAL1_ECOL6
AccessioniPrimary (citable) accession number: Q8FJS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.