Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8FHV4 (RLUB_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal large subunit pseudouridine synthase B

EC=5.4.99.22
Alternative name(s):
23S rRNA pseudouridine(2605) synthase
rRNA pseudouridylate synthase B
rRNA-uridine isomerase B
Gene names
Name:rluB
Ordered Locus Names:c1734
OrganismEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]
Taxonomic identifier199310 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA By similarity.

Catalytic activity

23S rRNA uridine(2605) = 23S rRNA pseudouridine(2605).

Sequence similarities

Belongs to the pseudouridine synthase RsuA family.

Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
   Biological processrRNA processing
   LigandRNA-binding
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpseudouridine synthesis

Inferred from electronic annotation. Source: InterPro

rRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

pseudouridine synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Ribosomal large subunit pseudouridine synthase B
PRO_0000099984

Regions

Domain3 – 7573S4 RNA-binding

Sites

Active site1101Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FHV4 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: C31DF8F0C5CB5578

FASTA29132,725
        10         20         30         40         50         60 
MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIAKLGD RVEITPGLKI RIDGHLISVR 

        70         80         90        100        110        120 
ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG ARWIAVGRLD VNTCGLLLFT 

       130        140        150        160        170        180 
TDGELANRLM HPSREVEREY AVRVFGQVDD AKLRDLSRGV QLEDGPAAFK TIKFSGGEGI 

       190        200        210        220        230        240 
NQWYNVTLTE GRNREVRRLW EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL 

       250        260        270        280        290 
RELVELPPET SSKVAVEKDR RRMKANQIRR AVKRHSQVSG GRRSGGRNNN G 

« Hide

References

[1]"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli."
Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R.
Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014075 Genomic DNA. Translation: AAN80200.1.
RefSeqNP_753638.1. NC_004431.1.

3D structure databases

ProteinModelPortalQ8FHV4.
SMRQ8FHV4. Positions 2-240.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING199310.c1734.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN80200; AAN80200; c1734.
GeneID1035518.
KEGGecc:c1734.
PATRIC18281390. VBIEscCol75197_1614.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000044954.
KOK06178.
OMAARSQKIR.
OrthoDBEOG6130DV.

Enzyme and pathway databases

BioCycECOL199310:C1734-MONOMER.

Family and domain databases

Gene3D3.10.290.10. 1 hit.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTSM00363. S4. 1 hit.
[Graphical view]
SUPFAMSSF55120. SSF55120. 1 hit.
TIGRFAMsTIGR00093. TIGR00093. 1 hit.
PROSITEPS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRLUB_ECOL6
AccessionPrimary (citable) accession number: Q8FHV4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families