Q8FHV4 (RLUB_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal large subunit pseudouridine synthase B
EC=5.4.99.22

Alternative name(s):
23S rRNA pseudouridine(2605) synthase
rRNA pseudouridylate synthase B
rRNA-uridine isomerase B

Gene names

Name:	rluB
Ordered Locus Names:	c1734

Organism

Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

199310 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA By similarity.
Catalytic activity	23S rRNA uridine(2605) = 23S rRNA pseudouridine(2605).
Sequence similarities	Belongs to the pseudouridine synthase RsuA family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	RNA-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Ribosomal large subunit pseudouridine synthase B		PRO_0000099984
Regions
	Domain	3 – 75	73	S4 RNA-binding
Sites
	Active site	110	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FHV4 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C31DF8F0C5CB5578	FASTA	291	32,725
	10 20 30 40 50 60 MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIAKLGD RVEITPGLKI RIDGHLISVR 70 80 90 100 110 120 ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG ARWIAVGRLD VNTCGLLLFT 130 140 150 160 170 180 TDGELANRLM HPSREVEREY AVRVFGQVDD AKLRDLSRGV QLEDGPAAFK TIKFSGGEGI 190 200 210 220 230 240 NQWYNVTLTE GRNREVRRLW EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL 250 260 270 280 290 RELVELPPET SSKVAVEKDR RRMKANQIRR AVKRHSQVSG GRRSGGRNNN G

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN80200.1.
RefSeq	NP_753638.1. NC_004431.1.
3D structure databases
ProteinModelPortal	Q8FHV4.
SMR	Q8FHV4. Positions 2-240.
ModBase	Search...
Protein-protein interaction databases
STRING	199310.c1734.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN80200; AAN80200; c1734.
GeneID	1035518.
KEGG	ecc:c1734.
PATRIC	18281390. VBIEscCol75197_1614.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000044954.
KO	K06178.
OMA	PRGGWIE.
ProtClustDB	PRK10700.
Family and domain databases
Gene3D	3.10.290.10. 1 hit.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR000748. PsdUridine_synth_RsuA/RluB/E/F. IPR018496. PsdUridine_synth_RsuA/RluB_CS. IPR006145. PsdUridine_synth_RsuA/RluD. IPR002942. S4_RNA-bd. [Graphical view]
Pfam	PF00849. PseudoU_synth_2. 1 hit. PF01479. S4. 1 hit. [Graphical view]
SMART	SM00363. S4. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00093. TIGR00093. 1 hit.
PROSITE	PS01149. PSI_RSU. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLUB_ECOL6

Accession

Primary (citable) accession number: Q8FHV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program