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Reviewed, UniProtKB/Swiss-Prot Q8FHH1 (MAO1_ECOL6)

Last modified June 16, 2009. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38
Gene names
Name: sfcA
Ordered Locus Names: c1912
OrganismEscherichia coli O6 [Complete proteome] [HAMAP]
Taxonomic identifier217992 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length565 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619

Cofactor

Divalent metal cations. Prefers magnesium or manganese By similarity.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the malic enzymes family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 565565NAD-dependent malic enzyme HAMAP MF_01619
PRO_0000160217

Sites

Active site1041Proton donor By similarity
Active site1751Proton acceptor By similarity
Metal binding2461Divalent metal cation By similarity
Metal binding2471Divalent metal cation By similarity
Metal binding2701Divalent metal cation By similarity
Binding site1571NAD By similarity
Binding site2701NAD By similarity
Binding site4181NAD By similarity
Site2701Important for activity By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q8FHH1-1 [UniParc].

Last modified December 6, 2005. Version 2.
Checksum: E0F0F413E83F8F3A

FASTA56563,202
        10         20         30         40         50         60 
MEPKTKKQRS LYIPYAGPVL LEFPLLNKGS AFSMEERRNF NLLGLLPEVV ETIEEQAERA 

        70         80         90        100        110        120 
WIQYQGFKTE IDKHIYLRNI QDTNETLFYR LVNNHLDEMM PVIYTPTVGA ACERFSEIYR 

       130        140        150        160        170        180 
RSRGVFISYQ NRHNMDDILQ NVPNHNIKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 

       190        200        210        220        230        240 
ACGGISPAYT LPVVLDVGTN NQQLLNDPLY MGWRNPRITD DEYYEFVDEF IQAVKQRWPD 

       250        260        270        280        290        300 
VLLQFEDFAQ KNAMPLLNRY RNEICSFNDD IQGTAAVTVG TLIAASRAAG GQLSEKKIVF 

       310        320        330        340        350        360 
LGAGSAGCGI AEMIIAQTQR EGLSEEAARQ KVFMVDRFGL LTDKMPNLLP FQTKLVQKRE 

       370        380        390        400        410        420 
NLSDWDTDSD VLSLLDVVRN VKPDILIGVS GQTGLFTEEI IREMHKHCPR PIVMPLSNPT 

       430        440        450        460        470        480 
SRVEATPQDI IAWTEGNALV ATGSPFNPVV WKDKIYPIAQ CNNAFIFPGI GLGVIASGAS 

       490        500        510        520        530        540 
RITDEMLMSA SEMLAQYSPL VLNGEGLVLP ELKDIQKVSR AIAFAVGKMA QQQGVAVKTS 

       550        560 
AEALQQAIDD NFWHAEYRDY RRTSI

« Hide

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References

[1]"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli."
Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R.
Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

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Cross-references

Sequence databases

AE014075 Genomic DNA. Translation: AAN80371.1. Different initiation.
RefSeqNP_753809.1.

3D structure databases

HSSPHSSP built from PDB template 1GZ3 based on UniProtKB P23368.
ModBaseSearch...

Genome annotation databases

GeneID1035818.
GenomeReviewsGene locus c1912 in contig AE014075_GR.
KEGGecc:c1912.
NMPDRfig|199310.1.peg.1852.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ8FHH1.
OMAQ8FHH1. IQDNNET.

Enzyme and pathway databases

BRENDA1.1.1.38. 292881.

Family and domain databases

HAMAPMF_01619.
[Tree]
InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N.
IPR012302. Malic_NAD_bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PRINTSPR00072. MALOXRDTASE.
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMAO1_ECOL6
AccessionPrimary (citable) accession number: Q8FHH1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 16, 2009
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents