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Protein

Dihydrofolate reductase FolM

Gene

folM

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reduction of dihydrofolate to tetrahydrofolate.By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei152 – 1521Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. one-carbon metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciECOL199310:C1998-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase FolM (EC:1.5.1.3)
Short name:
DHFR
Gene namesi
Name:folM
Ordered Locus Names:c1998
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001410 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Dihydrofolate reductase FolMPRO_0000339395Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi199310.c1998.

Structurei

3D structure databases

ProteinModelPortaliQ8FHB1.
SMRiQ8FHB1. Positions 7-240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK13938.
OMAiAVIHNAS.
OrthoDBiEOG6WDSG3.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8FHB1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKTQSLPIL ITGGGRRIGL ALAWHFINQK QPVIVSYRTH YPAIDGLIKA
60 70 80 90 100
GAQCIQADFS TNDGVMAFAD EVLKSTHGLR AILHNASAWM AEKPGAPLTD
110 120 130 140 150
VLACMMQIHV NTPYLLNHAL ERLLRGHGHA ASDIIHFTDY VVERGSDKHI
160 170 180 190 200
AYAASKAALD NMTRSFARKL APEVKVNSIA PSLILFNEHD DAEYRQQALN
210 220 230 240
KSLMKTAPGE KEVIDLVDYL LTSCFVTGRS LPLDGGRHLR
Length:240
Mass (Da):26,348
Last modified:March 1, 2003 - v1
Checksum:i72A9A8E1D7EB6C4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN80458.1.
RefSeqiNP_753893.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN80458; AAN80458; c1998.
GeneIDi1036028.
KEGGiecc:c1998.
PATRICi18281893. VBIEscCol75197_1865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN80458.1.
RefSeqiNP_753893.1. NC_004431.1.

3D structure databases

ProteinModelPortaliQ8FHB1.
SMRiQ8FHB1. Positions 7-240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi199310.c1998.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN80458; AAN80458; c1998.
GeneIDi1036028.
KEGGiecc:c1998.
PATRICi18281893. VBIEscCol75197_1865.

Phylogenomic databases

KOiK13938.
OMAiAVIHNAS.
OrthoDBiEOG6WDSG3.

Enzyme and pathway databases

BioCyciECOL199310:C1998-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFT073 / ATCC 700928 / UPEC.

Entry informationi

Entry nameiFOLM_ECOL6
AccessioniPrimary (citable) accession number: Q8FHB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 1, 2003
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.