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Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive

Gene

aroH

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).By similarity

Catalytic activityi

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Pathwayi: chorismate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase (c1220)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Quinate/shikimate dehydrogenase (ydiB), Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciECOL199310:C2100-MONOMER.
UniPathwayiUPA00053; UER00084.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (EC:2.5.1.54)
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesi
Name:aroH
Ordered Locus Names:c2100
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001410 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitivePRO_0000140841Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi199310.c2100.

Structurei

3D structure databases

ProteinModelPortaliQ8FH32.
SMRiQ8FH32. Positions 2-347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I DAHP synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105E99. Bacteria.
COG0722. LUCA.
HOGENOMiHOG000220501.
KOiK01626.
OMAiPCLSWED.
OrthoDBiEOG63JR9W.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view]
PANTHERiPTHR21225. PTHR21225. 1 hit.
PfamiPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsiTIGR00034. aroFGH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FH32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRTDELRTA RIESLVTPAE LALRYPVTPG VATHVTDSRR RIEKILNGED
60 70 80 90 100
KRLLVIIGPC SIHDLTAAME YATRLQSLRN QYQSRLEIVM RTYFEKPRTV
110 120 130 140 150
VGWKGLISDP DLNGSYRVNH GLELARKLLL QVNELGVPTA TEFLDMVTGQ
160 170 180 190 200
FIADLISWGA IGARTTESQI HREMASALSC PVGFKNGTDG NTRIAVDAIR
210 220 230 240 250
AARASHMFLS PDKNGQMTIY QTSGNPYGHI IMRGGKKPNY HADDIAAACD
260 270 280 290 300
TLHEFDLPEH LVVDFSHGNC QKQHRRQLEV CEDICQQIRN GSTAIAGIMA
310 320 330 340
ESFLREGTQK IVGGQPLTYG QSITDPCLGW EDTERLVEKL ASAVDTRF
Length:348
Mass (Da):38,705
Last modified:March 1, 2003 - v1
Checksum:i52C3D89F85B226E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN80560.1.
RefSeqiWP_001082226.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN80560; AAN80560; c2100.
KEGGiecc:c2100.
PATRICi18282099. VBIEscCol75197_1966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN80560.1.
RefSeqiWP_001082226.1. NC_004431.1.

3D structure databases

ProteinModelPortaliQ8FH32.
SMRiQ8FH32. Positions 2-347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi199310.c2100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN80560; AAN80560; c2100.
KEGGiecc:c2100.
PATRICi18282099. VBIEscCol75197_1966.

Phylogenomic databases

eggNOGiENOG4105E99. Bacteria.
COG0722. LUCA.
HOGENOMiHOG000220501.
KOiK01626.
OMAiPCLSWED.
OrthoDBiEOG63JR9W.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00084.
BioCyciECOL199310:C2100-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view]
PANTHERiPTHR21225. PTHR21225. 1 hit.
PfamiPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsiTIGR00034. aroFGH. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFT073 / ATCC 700928 / UPEC.

Entry informationi

Entry nameiAROH_ECOL6
AccessioniPrimary (citable) accession number: Q8FH32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2003
Last modified: November 11, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.