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Q8FGN6

- OTSA_ECOL6

UniProt

Q8FGN6 - OTSA_ECOL6

Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 2 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.By similarity

    Catalytic activityi

    UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101Glucose-6-phosphateBy similarity
    Binding sitei77 – 771Glucose-6-phosphateBy similarity
    Sitei86 – 861Involved in alpha anomer selectivityBy similarity
    Binding sitei131 – 1311Glucose-6-phosphateBy similarity
    Sitei156 – 1561Involved in alpha anomer selectivityBy similarity
    Binding sitei263 – 2631UDP-glucoseBy similarity
    Binding sitei268 – 2681UDP-glucoseBy similarity
    Binding sitei301 – 3011Glucose-6-phosphateBy similarity
    Binding sitei340 – 3401UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

    GO - Biological processi

    1. trehalose biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00299.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
    Alternative name(s):
    Osmoregulatory trehalose synthesis protein A
    Trehalose-6-phosphate synthase
    UDP-glucose-glucosephosphate glucosyltransferase
    Gene namesi
    Name:otsA
    Ordered Locus Names:c2310
    OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
    Taxonomic identifieri199310 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001410: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 474474Alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000348899Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi199310.c2310.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8FGN6.
    SMRiQ8FGN6. Positions 2-457.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni22 – 232UDP-glucose bindingBy similarity
    Regioni366 – 3705UDP-glucose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 20 family.Curated

    Phylogenomic databases

    HOGENOMiHOG000191478.
    KOiK00697.
    OrthoDBiEOG6CCH1M.

    Family and domain databases

    InterProiIPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view]
    PfamiPF00982. Glyco_transf_20. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8FGN6-1 [UniParc]FASTAAdd to Basket

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    MSRLVVVSNR IAPPDEHAAS AGGLAVGILG ALKAAGGLWF GWSGETGNED    50
    QPLKKVKKGN ITWASFNLSE QDLDEYYNKF SNAVLWPAFH YRLDLVQFQR 100
    PAWDGYLRVN ALLADKLLPL LQDDDIIWIH DYHLLPFAHE LRKRGVNNRI 150
    GFFLHIPFPT PEIFNALPTY DTLLEQLCDY DLLGFQTEND RLAFLDCLSN 200
    LTRVTTRSAK SHTACGKAFR TEVYPIGIEP KEIAKQAAGP LPPKLAQLKA 250
    ELKNVQNIFS VERLDYSKGL PERFLAYEAL LEKYPQHHGK IRYTQIAPTS 300
    RGDVQAYQDI RHQLENEAGR INGKYGQLGW TPLYYLNQHF DRKLLMKIFR 350
    YSDVGLVTPL RDGMNLVAKE YVAAQDPANP GVLVLSQFAG AANELTSALI 400
    VNPYDRDEVA AALDRALTMS LAERISRHAE MLDVIVKNDI NHWQECFISD 450
    LKQIVPRSAE SQQRDKVATF PKLA 474
    Length:474
    Mass (Da):53,528
    Last modified:September 2, 2008 - v2
    Checksum:iC81B480CF458A7FE
    GO

    Sequence cautioni

    The sequence AAN80769.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014075 Genomic DNA. Translation: AAN80769.1. Different initiation.
    RefSeqiNP_754202.1. NC_004431.1.

    Genome annotation databases

    EnsemblBacteriaiAAN80769; AAN80769; c2310.
    GeneIDi1036913.
    KEGGiecc:c2310.
    PATRICi18282501. VBIEscCol75197_2167.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014075 Genomic DNA. Translation: AAN80769.1 . Different initiation.
    RefSeqi NP_754202.1. NC_004431.1.

    3D structure databases

    ProteinModelPortali Q8FGN6.
    SMRi Q8FGN6. Positions 2-457.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 199310.c2310.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN80769 ; AAN80769 ; c2310 .
    GeneIDi 1036913.
    KEGGi ecc:c2310.
    PATRICi 18282501. VBIEscCol75197_2167.

    Phylogenomic databases

    HOGENOMi HOG000191478.
    KOi K00697.
    OrthoDBi EOG6CCH1M.

    Enzyme and pathway databases

    UniPathwayi UPA00299 .

    Family and domain databases

    InterProi IPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view ]
    Pfami PF00982. Glyco_transf_20. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CFT073 / ATCC 700928 / UPEC.

    Entry informationi

    Entry nameiOTSA_ECOL6
    AccessioniPrimary (citable) accession number: Q8FGN6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 57 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3