Reviewed, UniProtKB/Swiss-Prot Q8FFN4 (GLPB_ECOL6)

Names and origin

Protein names

Recommended name:
    Anaerobic glycerol-3-phosphate dehydrogenase subunit B
      Short name=Anaerobic G-3-P dehydrogenase subunit B
      Short name=Anaerobic G3Pdhase B
    EC=1.1.5.3

Gene names

Name:	glpB
Ordered Locus Names:	c2783

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor By similarity.
Catalytic activity	sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol. HAMAP MF_00753
Cofactor	FMN By similarity.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1. HAMAP MF_00753
Subunit structure	Composed of a catalytic glpA/B dimer and of membrane bound glpC By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity. Note: Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase By similarity.
Sequence similarities	Belongs to the anaerobic G-3-P dehydrogenase subunit B family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Anaerobic glycerol-3-phosphate dehydrogenase subunit B HAMAP MF_00753		PRO_0000204560

Sequences

	Sequence		Length	Mass (Da)
	Q8FFN4-1 [UniParc]. Last modified March 29, 2004. Version 2. Checksum: 21E116E3644E0EDD	FASTA	419	45,398
	10 20 30 40 50 60 MRFDTVIMGG GLAGLLCGLQ LQKHGLRCAI VTRGQSALHF SSGSLDLLSH LPDGQPVTDI 70 80 90 100 110 120 HSGLESLRQQ APAHPYTLLG PQRVLDLACQ AQALIAESGA QLQGSVELAH QRITPLGTLR 130 140 150 160 170 180 STWLSSPEVP VWPLPAKKIC VVGISGLMDF QAHLAAASLR ELDLAVETAE IELPELDVLR 190 200 210 220 230 240 NNATEFRAVN IARFLDNEEN WPLIIDALIP VANTCEMILM PACFGLADDK LWRWLNEKLP 250 260 270 280 290 300 CSLMLLPTLP PSVLGIRLQN QLQRQFVRQG GVWMPGDEVK KVTCKNGVVN EIWTRNHADI 310 320 330 340 350 360 PLRPRFVVLA SGSFFSGGLV AERDGIREPI LGLDVLQTAT RGEWYKGDFF APQPWQQFGV 370 380 390 400 410 TTDEALRPSQ AGQTIENLFA IGSVLGGFDP IAQGCGGGVC AVSALHVAQQ IAQRAGGQQ

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014075 Genomic DNA. Translation: AAN81237.1. Different initiation.
RefSeq	NP_754669.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1038299.
GenomeReviews	Gene locus c2783 in contig AE014075_GR.
KEGG	ecc:c2783.
NMPDR	fig|199310.1.peg.2712.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8FFN4.
OMA	Q8FFN4. TWLSQPF.
Enzyme and pathway databases
BRENDA	1.1.99.5. 292881.
Family and domain databases
HAMAP	MF_00753. [Tree]
InterPro	IPR009158. Anaerobic_glycerol3P_DH_bsu. IPR003953. FAD_bind2_N. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. [Graphical view]
PIRSF	PIRSF000141. Anaerobic_G3P_dh. 1 hit.
TIGRFAMs	TIGR03378. glycerol3P_GlpB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLPB_ECOL6

Accession

Primary (citable) accession number: Q8FFN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	March 29, 2004
Last modified:	June 16, 2009
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)