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Protein

5'-deoxynucleotidase YfbR

Gene

yfbR

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the strictly specific dephosphorylation of 2'-deoxyribonucleoside 5'-monophosphates.UniRule annotation

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
A 2'-deoxyribonucleoside 5'-monophosphate + H2O = a 2'-deoxyribonucleoside + phosphate.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Enzyme regulationi

Inhibited by both ribo- and deoxyribonucleoside di- and triphosphates.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei18Appears to be important in orienting the phosphate for catalysisUniRule annotation1
Metal bindingi33Divalent metal cation; via tele nitrogenUniRule annotation1
Binding sitei33SubstrateUniRule annotation1
Metal bindingi68Divalent metal cation; via tele nitrogenUniRule annotation1
Metal bindingi69Divalent metal cationUniRule annotation1
Binding sitei69SubstrateUniRule annotation1
Metal bindingi137Divalent metal cationUniRule annotation1
Binding sitei137SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL199310:C2832-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
5'-deoxynucleotidase YfbRUniRule annotation (EC:3.1.3.89UniRule annotation)
Alternative name(s):
5'-deoxyribonucleotidaseUniRule annotation
Nucleoside 5'-monophosphate phosphohydrolaseUniRule annotation
Gene namesi
Name:yfbRUniRule annotation
Ordered Locus Names:c2832
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001410 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950511 – 1995'-deoxynucleotidase YfbRAdd BLAST199

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi199310.c2832.

Structurei

3D structure databases

ProteinModelPortaliQ8FFJ5.
SMRiQ8FFJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 142HDPROSITE-ProRule annotationAdd BLAST113

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 19Substrate bindingUniRule annotation2
Regioni77 – 80Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the 5DNU family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DK2. Bacteria.
COG1896. LUCA.
HOGENOMiHOG000276964.
KOiK08722.
OMAiNQSHFFA.

Family and domain databases

CDDicd00077. HDc. 1 hit.
HAMAPiMF_01100. 5DNU. 1 hit.
InterProiView protein in InterPro
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR022971. YfbR.
PfamiView protein in Pfam
PF13023. HD_3. 1 hit.
SMARTiView protein in SMART
SM00471. HDc. 1 hit.
PROSITEiView protein in PROSITE
PS51831. HD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FFJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQSHFFAHL SRLKLINRWP LMRNVRTENV SEHSLQVAMV AHALAAIKNR
60 70 80 90 100
KFGGNVNAER IALLAMYHDA SEVLTGDLPT PVKYFNSQIA QEYKAIEKIA
110 120 130 140 150
QQKLVDMVPE ELQDIFAPLI DEHAYSDEEK SLVKQADALC AYLKCLEELA
160 170 180 190
AGNNEFLLAK TRLEATLEAR RSQEMDYFME VFVPSFHLSL DEISQDSPL
Length:199
Mass (Da):22,666
Last modified:March 1, 2003 - v1
Checksum:i1A6EBF69F00DA238
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN81286.1.
RefSeqiWP_000813854.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN81286; AAN81286; c2832.
KEGGiecc:c2832.

Similar proteinsi

Entry informationi

Entry namei5DNU_ECOL6
AccessioniPrimary (citable) accession number: Q8FFJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: March 1, 2003
Last modified: September 27, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families