Q8FEF7 (QUEF_ECOL6) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 73.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: NADPH-dependent 7-cyano-7-deazaguanine reductase EC=1.7.1.13 Alternative name(s): 7-cyano-7-carbaguanine reductase NADPH-dependent nitrile oxidoreductase PreQ(0) reductase | ||||
| Gene names |
| ||||
| Organism | Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 199310 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › ![]() |
Protein attributes
| Sequence length | 282 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00817 |
| Catalytic activity | 7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00817 |
| Pathway | tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00817 |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Queuosine biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological_process | queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP |
| Cellular_component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP preQ1 synthase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 282 | 282 | NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00817 | PRO_0000163033 | |||
Sequences
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References
| [1] | "Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFT073 / ATCC 700928 / UPEC. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE014075 Genomic DNA. Translation: AAN81807.1. |
| RefSeq | NP_755237.1. NC_004431.1. |
3D structure databases | |
| ProteinModelPortal | Q8FEF7. |
| SMR | Q8FEF7. Positions 21-282. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 199310.c3360. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | AAN81807; AAN81807; c3360. |
| GeneID | 1039203. |
| KEGG | ecc:c3360. |
| PATRIC | 18284552. VBIEscCol75197_3165. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HOG000273755. |
| KO | K06879. |
| OMA | ERIFCDL. |
| ProtClustDB | PRK11792. |
Enzyme and pathway databases | |
| UniPathway | UPA00392. |
Family and domain databases | |
| HAMAP | MF_00817. QueF_type2. |
| InterPro | IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view] |
| Pfam | PF01227. GTP_cyclohydroI. 1 hit. [Graphical view] |
| PIRSF | PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit. |
| TIGRFAMs | TIGR03138. QueF. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | QUEF_ECOL6 | ||||||||
| Accession | Primary (citable) accession number: Q8FEF7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
