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Q8FE30

- GSHB_ECOL6

UniProt

Q8FE30 - GSHB_ECOL6

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Protein
Glutathione synthetase
Gene
gshB, c3533
Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi281 – 2811Magnesium or manganese By similarity
Metal bindingi283 – 2831Magnesium or manganese By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi151 – 20757ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutathione synthase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL199310:C3533-MONOMER.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Alternative name(s):
GSH synthetase
Short name:
GSH-S
Short name:
GSHase
Glutathione synthase
Gene namesi
Name:gshB
Ordered Locus Names:c3533
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001410: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Glutathione synthetaseUniRule annotation
PRO_0000197466Add
BLAST

Proteomic databases

PRIDEiQ8FE30.

Interactioni

Protein-protein interaction databases

STRINGi199310.c3533.

Structurei

3D structure databases

ProteinModelPortaliQ8FE30.
SMRiQ8FE30. Positions 1-314.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 310186ATP-grasp
Add
BLAST

Sequence similaritiesi

Contains 1 ATP-grasp domain.

Phylogenomic databases

HOGENOMiHOG000265022.
KOiK01920.
OMAiDWKIARA.
OrthoDBiEOG6WMHWB.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8FE30-1 [UniParc]FASTAAdd to Basket

« Hide

MIKLGIVMDP IANINIKKDS SFAMLLEAQR RGYELHYMEM ADLYLINGEA    50
RARTRTLSVE QNYDKWYEFT GEQDLPLADL DVILMRKDPP FDTEFIYSTY 100
ILERAEDKGT LIVNKPQSLR DCNEKLFTAW FSDLTPETLV TRNKAQLKAF 150
WEKHSDIILK PLDGMGGASI FRVKEGDPNL GVIAETLTEH GTRYCMAQNY 200
LPAIKDGDKR VLVVDGEPVP YCLARIPQGG ETRGNLAAGG RGEPRPLTES 250
DWKIARQIGP TLKEKGLIFV GLDIIGDRLT EINVTSPTCI REIEAEFPVS 300
ITGMLMDAIE ARLQQQ 316
Length:316
Mass (Da):35,615
Last modified:March 1, 2003 - v1
Checksum:iF2F48A07427CB27A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014075 Genomic DNA. Translation: AAN81981.1.
RefSeqiNP_755408.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN81981; AAN81981; c3533.
GeneIDi1039448.
KEGGiecc:c3533.
PATRICi18284886. VBIEscCol75197_3328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014075 Genomic DNA. Translation: AAN81981.1 .
RefSeqi NP_755408.1. NC_004431.1.

3D structure databases

ProteinModelPortali Q8FE30.
SMRi Q8FE30. Positions 1-314.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 199310.c3533.

Proteomic databases

PRIDEi Q8FE30.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN81981 ; AAN81981 ; c3533 .
GeneIDi 1039448.
KEGGi ecc:c3533.
PATRICi 18284886. VBIEscCol75197_3328.

Phylogenomic databases

HOGENOMi HOG000265022.
KOi K01920.
OMAi DWKIARA.
OrthoDBi EOG6WMHWB.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .
BioCyci ECOL199310:C3533-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPi MF_00162. GSH_S.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
Pfami PF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view ]
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01380. glut_syn. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFT073 / ATCC 700928 / UPEC.

Entry informationi

Entry nameiGSHB_ECOL6
AccessioniPrimary (citable) accession number: Q8FE30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2003
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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