Q8FDN6 (MASZ_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	c3705

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Cofactor	Magnesium By similarity. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 723	722	Malate synthase G HAMAP MF_00641		PRO_0000166887
Sites
	Active site	338	1	Proton acceptor By similarity
	Active site	631	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FDN6 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: AAF740E5FE038F6F	FASTA	723	80,440
	10 20 30 40 50 60 MSQTITQGRL RIDANFKRFV DEEVLPGVEL DAAAFWHNVD EIVHDLAPEN RQLLAERDRI 70 80 90 100 110 120 QAALDEWHRS NPGPVKDKAA YKSFLRELGY LVPQPDHVTV ETTGIDSEIT SQAGPQLVVP 130 140 150 160 170 180 AMNARYALNA ANARWGSLYD ALYGSDIIPQ EGAMVSGYDP QRGEQVIAWV RRFLDESLPL 190 200 210 220 230 240 ENGSYQDVVA FKVVDKQLRI QLKNGKETTL RTPAQFVGYR GDTAAPTCIL LKNNGLHIEL 250 260 270 280 290 300 QIDANGRIGK DDSAHINDVI VEAAISTILD CEDSVAAVDA EDKILLYRNL LGLMQGTLQE 310 320 330 340 350 360 KMEKNGRQIV RKLNDDRQYT AADGSEISLH GRSLLFIRNV GHLMTIPVIW DSEGNEIPEG 370 380 390 400 410 420 ILDGVMTGAI ALYDLKVQKN SRTGSVYIVK PKMHGPQEVA FANKLFSRVE TMLGMAPNTL 430 440 450 460 470 480 KMGIMDEERR TSLNLRSCIA QARNRVAFIN TGFLDRTGDE MHSVMEAGPM LRKNQMKSTP 490 500 510 520 530 540 WIKAYERNNV LSGLFCGLRG KAQIGKGMWA MPDLMADMYS QKGDQLRAGA NTAWVPSPTA 550 560 570 580 590 600 ATLHALHYHQ TNVQSVQANI AQTEFNAEFE PLLDDLLTIP VAENANWSVE EIQQELDNNV 610 620 630 640 650 660 QGILGYVVRW VEQGIGCSKV PDIHNVALME DRATLRISSQ HIANWLRHGI LTKEQVQASL 670 680 690 700 710 720 ENMAKVVDQQ NAGDPAYRPM AGNFANSCAF KAASDLIFLG VKQPNGYTEP LLHAWRLREK ESH

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN82151.1.
RefSeq	NP_755578.1. NC_004431.1.
3D structure databases
ProteinModelPortal	Q8FDN6.
SMR	Q8FDN6. Positions 1-723.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000043965; EBESCP00000042314; EBESCG00000043015.
GeneID	1039747.
GenomeReviews	Gene locus c3705 in contig AE014075_GR.
KEGG	ecc:c3705.
NMPDR	fig|199310.1.peg.3621.
PATRIC	18285199. VBIEscCol75197_3483.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010684.
HOGENOM	HBG350005.
OMA	ENANWSV.
ProtClustDB	PRK02999.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_ECOL6

Accession

Primary (citable) accession number: Q8FDN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program