Reviewed, UniProtKB/Swiss-Prot Q8FCS8 (RTCA_ECOL6)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	c4197

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subunit structure	Homodimer; disulfide-linked By similarity. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156417
Sites
	Active site	308	1	By similarity
Amino acid modifications
	Disulfide bond	307		Interchain By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FCS8-1 [UniParc]. Last modified June 6, 2003. Version 2. Checksum: 38075DF4CA2CBC33	FASTA	338	35,949
	10 20 30 40 50 60 MKRMIALDGA QGEGGGQILR SALSLPMITG QPFTITGIRA GRAKPGLLRQ HLTAVKAAAE 70 80 90 100 110 120 ICRATVEGAE LGSQRLVFRP GTVRGGDYRF AIGSAGSCTL VLQTVLPALW FADGPSRVEV 130 140 150 160 170 180 SGGTDNPSAP PADFIRRVLE PLLARIGVHQ QTTLLRHGFY PAGGGVVATE VSPVASFNSL 190 200 210 220 230 240 QLGERGNIVQ MRGEVLLAGV PRHVAEREIA TLAGSFSLHE QNIHNLPRDQ GPGNTVSLEV 250 260 270 280 290 300 ESENITERFF VVGEKRVSAE VVAAQLVKEV KRYLASPAAV GEYLADQLVL PMALAGAGEF 310 320 330 TVAHPSCHLQ TNIAVVERFL PVRFSLIETD GVTRVSIE

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN82635.1. Different initiation.
RefSeq	NP_756061.1.
3D structure databases
SMR	Q8FCS8. Positions 4-338.
ModBase	Search...
Genome annotation databases
GeneID	1036815.
GenomeReviews	Gene locus c4197 in contig AE014075_GR.
KEGG	ecc:c4197.
NMPDR	fig|199310.1.peg.4104.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG683539.
OMA	EIDGSYG.
Enzyme and pathway databases
BRENDA	6.5.1.4. 292881.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR000228. RNA3'_term_phos_cycl-like. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR020723. RNA3'_term_phos_cycl_prd. IPR017770. RNA3'_term_phos_cycl_sub. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_ECOL6

Accession

Primary (citable) accession number: Q8FCS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	June 6, 2003
Last modified:	February 9, 2010
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)