Q8FAE1 (OTCC_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase, catabolic
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	arcB
Ordered Locus Names:	c5348

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2. HAMAP MF_01109
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.
Sequence caution	The sequence AAN83770.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Arginine metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Ornithine carbamoyltransferase, catabolic HAMAP MF_01109		PRO_0000112921
Regions
	Region	57 – 61	5	Carbamoyl phosphate binding By similarity
	Region	274 – 277	4	Ornithine binding By similarity
Sites
	Binding site	108	1	Carbamoyl phosphate By similarity
	Binding site	135	1	Carbamoyl phosphate By similarity
	Site	32	1	Important for structural integrity By similarity
	Site	148	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FAE1 [UniParc]. Last modified July 11, 2003. Version 2. Checksum: 67145B6F1F094C3D	FASTA	334	37,113
	10 20 30 40 50 60 MATSLKNRNF LKLLDYTPAE IQYLIDLAIN LKAAKKSGNE KQTLVGKNIA LIFEKSSTRT 70 80 90 100 110 120 RCAFEVAAFD QGAQVTYIGP SGSQIGHKES MKDTARVLGR MYDGIEYRGY GQNIVEELGE 130 140 150 160 170 180 FAGVPVWNGL TNEFHPTQIL ADLMTMLEHA PGKTLPELSF AYLGDARNNM GNSLMVGAAK 190 200 210 220 230 240 MGMDIRLVAP KSFWPDEALV TQCREIASVT GARITLTEDV EEGVYDVDFL YTDVWVSMGE 250 260 270 280 290 300 PKEAWAERVS LMAPYQINQQ VITATRNPEV KFMHCLPAFH NEHTTVGREI EMAYGLKGLE 310 320 330 VTEEVFESAH SIVFDEAENR MHTIKAVMVA TLGD

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN83770.1. Different initiation.
RefSeq	NP_757196.2. NC_004431.1.
3D structure databases
ProteinModelPortal	Q8FAE1.
SMR	Q8FAE1. Positions 7-332.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000041818; EBESCP00000040167; EBESCG00000040868.
GeneID	1037360.
GenomeReviews	Gene locus c5348 in contig AE014075_GR.
KEGG	ecc:c5348.
PATRIC	18288340. VBIEscCol75197_5011.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009122.
HOGENOM	HBG579429.
OMA	MTPYQIN.
PhylomeDB	Q8FAE1.
ProtClustDB	PRK02102.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
KO	K00611.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTCC_ECOL6

Accession

Primary (citable) accession number: Q8FAE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	July 11, 2003
Last modified:	January 25, 2012
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program