Q8F9L0 (FUMC_LEPIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate hydratase class II
Short name=Fumarase C
EC=4.2.1.2

Gene names

Name:	fumC
Synonyms:	fum
Ordered Locus Names:	LA_0185

Organism

Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)

Taxonomic identifier

189518 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743
Subunit structure	Homotetramer By similarity. HAMAP MF_00743
Subcellular location	Cytoplasm By similarity HAMAP MF_00743.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161283
Regions
	Region	128 – 131	4	B site By similarity
	Region	138 – 140	3	Substrate binding By similarity
Sites
	Binding site	99	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8F9L0 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: AE316E36BAB02FA8	FASTA	464	50,397
	10 20 30 40 50 60 MKTRIETDSM GEIAVDDSKY WGAQTERSLH HFHIGNDRFP REMIRALGIL KKSAAVVNAE 70 80 90 100 110 120 LGLLSEDKKK LIVQAADEVI SGKLDEHFPL SVWQTGSGTQ TNMNSNEVIS NRAIEIAGGV 130 140 150 160 170 180 KGSKKPIHPN DDVNKAQSSN DTFPTAMHIA AAEQLNQKLI PALIQLKETF KKKTDEFQNI 190 200 210 220 230 240 IKIGRTHLQD ATPLTLGQEF SGYVQQLEYN IARVKAVLPS VYRLALGGTA VGTGLNTHPQ 250 260 270 280 290 300 FAVKAAAQIA KETGLPFVSA ENKFEALAAH DSLVETSGVL KTIAASLMKI ANDIRWLSSG 310 320 330 340 350 360 PRCGIGEISI PENEPGSSIM PGKVNPTQSE QMTMVAAQVI ANDVAVNIGG ASGNFELNVF 370 380 390 400 410 420 KPLIIHNVLN SIRLLSDSCV SFEEHCARGI IPNKEKLNEH LNNSLMLVTA LNPHIGYDNA 430 440 450 460 AKIAKNAHKK GTTLKESGIE LGLLTSEQFD QWVLPEKMIH PSVD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing." Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P. Nature 422:888-893(2003) [PubMed: 12712204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 56601.
[2]	"Physical and genetic maps of the Leptospira interrogans serovar icterohaemorrhagiae strain Ictero No.1 chromosome and sequencing of a 19-kb region of the genome containing the 5S rRNA gene." Takahashi Y., Akase K., Hirano H., Fukunaga M. Gene 215:37-45(1998) [PubMed: 9666070] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-459. Strain: Ictero No.1 / Serogroup Icterohaemorrhagiae.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010300 Genomic DNA. Translation: AAN47384.1. AB010203 Genomic DNA. Translation: BAA24376.1.
PIR	T00129.
RefSeq	NP_710366.1. NC_004342.2.
3D structure databases
ProteinModelPortal	Q8F9L0.
SMR	Q8F9L0. Positions 4-459.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1149528.
GenomeReviews	Gene locus LA_0185 in contig AE010300_GR.
KEGG	lil:LA_0185.
PATRIC	22381209. VBILepInt91350_0184.
Phylogenomic databases
HOGENOM	HBG284369.
OMA	RIEKDTM.
PhylomeDB	Q8F9L0.
ProtClustDB	PRK00485.
Enzyme and pathway databases
BioCyc	LINT-130-01:LINT-130-01-000154-MONOMER. LINT189518:LA0185-MONOMER.
Family and domain databases
HAMAP	MF_00743. FumaraseC. [Tree]
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR022761. Lyase1_N. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KO	K01679.
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00979. FumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_LEPIN

Accession

Primary (citable) accession number: Q8F9L0
Secondary accession number(s): O50640

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program