Q8F7A0 (ISPD_LEPIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	LA_1048

Organism

Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)

Taxonomic identifier

189518 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_0000075582
Sites
	Site	22	1	Transition state stabilizer By similarity
	Site	29	1	Transition state stabilizer By similarity
	Site	160	1	Positions MEP for the nucleophilic attack By similarity
	Site	214	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8F7A0 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C02400F042434DFB	FASTA	238	26,663
	10 20 30 40 50 60 MKSLFLSEKI YVLILAGGTG SRMGSKIPKQ FLELNGEPIL IHSLKRFQNW GKQKRIVLVS 70 80 90 100 110 120 HFESIPKIES ICASYLENED RIVQGGENRH SSMLCGLSVL DFKDEDIILV HDAARPFVLA 130 140 150 160 170 180 DELDSLCEKV RSDGIATLAS RTSETVLEEL NGKTVSFLDR EHVWFMKTPQ GIRGDVLKEL 190 200 210 220 230 LTFSVDSIPT DLCSWALTFG KTSSIVESNP LNLKITRKED LDLAEVFSSL FQKISSDI

References

[1]

"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing." Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P. Nature 422:888-893(2003) [PubMed: 12712204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 56601.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010300 Genomic DNA. Translation: AAN48247.1.
RefSeq	NP_711229.1. NC_004342.2.
3D structure databases
ProteinModelPortal	Q8F7A0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1150391.
GenomeReviews	Gene locus LA_1048 in contig AE010300_GR.
KEGG	lil:LA_1048.
PATRIC	22382935. VBILepInt91350_1044.
Phylogenomic databases
HOGENOM	HBG672839.
OMA	REQQDFW.
PhylomeDB	Q8F7A0.
ProtClustDB	CLSK573749.
Enzyme and pathway databases
BioCyc	LINT189518:LA1048-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_LEPIN

Accession

Primary (citable) accession number: Q8F7A0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program