Reviewed, UniProtKB/Swiss-Prot Q8F6R2 (CARA_LEPIN)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	LA_1239

Organism

Leptospira interrogans [Complete proteome] [HAMAP]

Taxonomic identifier

173 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112289
Regions
	Domain	177 – 363	187	Glutamine amidotransferase type-1
	Region	1 – 173	173	CPSase HAMAP MF_01209
Sites
	Active site	253	1	Nucleophile By similarity
	Active site	336	1	By similarity
	Active site	338	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8F6R2-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 58BD2BB8F43D0BEE	FASTA	363	40,261
	10 20 30 40 50 60 MMKAFLVLDN GTIFEGESFG YETESVGEIV FNTSMAGYQE ILTDPSYCNQ IITLTYPMIG 70 80 90 100 110 120 NYGIHPDNME SSKIQASGLI VKEYVDLPSN FKSEKTLSQF LKEYKIPAIQ GIDTRKLTRF 130 140 150 160 170 180 IRTNGSPNGG IFVASEYSPS FLEKVKSFPG IINADLAKVV TTSSKYIFGT HTGKKFKLAV 190 200 210 220 230 240 YDYGVKTNIL RLLDANGFAV TVYPAKTPSE EIMKEGTDAF FLSNGPGDPA PLDYAIASTQ 250 260 270 280 290 300 KIMEKRYPLF GICLGHQIIG LSLGKKTEKM KFGHRGGNQP VKNLETGQVE ITSQNHGFAV 310 320 330 340 350 360 IDDQKQDEPI SFLNLNDHTV EGILKSGYPL LTVQYHPESA PGPNDSRYLF QKFYDLVEKT KKG

References

[1]

"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing." Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P. Nature 422:888-893(2003) [PubMed: 12712204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 56601 / Serogroup Icterohaemorrhagiae / Serovar lai.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010300 Genomic DNA. Translation: AAN48438.1.
RefSeq	NP_711420.1.
3D structure databases
SMR	Q8F6R2. Positions 1-362.
ModBase	Search...
Genome annotation databases
GeneID	1150582.
GenomeReviews	Gene locus LA_1239 in contig AE010300_GR.
KEGG	lil:LA1239.
NMPDR	fig|189518.1.peg.1239.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286341.
OMA	EADIPFF.
Enzyme and pathway databases
BioCyc	LINT189518:LA1239-MONOMER.
BRENDA	6.3.5.5. 258108.
Family and domain databases
HAMAP	MF_01209_B. CPSase_S_chain_B. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_LEPIN

Accession

Primary (citable) accession number: Q8F6R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)