Q8F6R2 (CARA_LEPIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbamoyl-phosphate synthase small chain
EC=6.3.5.5

Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	LA_1239

Organism

Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)

Taxonomic identifier

189518 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the CarA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112289
Regions
	Domain	176 – 362	187	Glutamine amidotransferase type-1
	Region	1 – 172	172	CPSase HAMAP MF_01209
Sites
	Active site	252	1	Nucleophile By similarity
	Active site	335	1	By similarity
	Active site	337	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8F6R2 [UniParc]. Last modified August 10, 2010. Version 2. Checksum: ECB2DC06136069AC	FASTA	362	40,130
	10 20 30 40 50 60 MKAFLVLDNG TIFEGESFGY ETESVGEIVF NTSMAGYQEI LTDPSYCNQI ITLTYPMIGN 70 80 90 100 110 120 YGIHPDNMES SKIQASGLIV KEYVDLPSNF KSEKTLSQFL KEYKIPAIQG IDTRKLTRFI 130 140 150 160 170 180 RTNGSPNGGI FVASEYSPSF LEKVKSFPGI INADLAKVVT TSSKYIFGTH TGKKFKLAVY 190 200 210 220 230 240 DYGVKTNILR LLDANGFAVT VYPAKTPSEE IMKEGTDAFF LSNGPGDPAP LDYAIASTQK 250 260 270 280 290 300 IMEKRYPLFG ICLGHQIIGL SLGKKTEKMK FGHRGGNQPV KNLETGQVEI TSQNHGFAVI 310 320 330 340 350 360 DDQKQDEPIS FLNLNDHTVE GILKSGYPLL TVQYHPESAP GPNDSRYLFQ KFYDLVEKTK KG

References

[1]

"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing." Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P. Nature 422:888-893(2003) [PubMed: 12712204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 56601.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010300 Genomic DNA. Translation: AAN48438.2.
RefSeq	NP_711420.2. NC_004342.2.
3D structure databases
ProteinModelPortal	Q8F6R2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1150582.
GenomeReviews	Gene locus LA_1239 in contig AE010300_GR.
KEGG	lil:LA_1239.
NMPDR	fig|189518.1.peg.1239.
PATRIC	22383329. VBILepInt91350_1239.
Phylogenomic databases
HOGENOM	HBG286341.
OMA	GWNDEDS.
ProtClustDB	PRK12564.
Enzyme and pathway databases
BioCyc	LINT189518:LA1239-MONOMER.
Family and domain databases
HAMAP	MF_01209. CPSase_S_chain. [Tree]
InterPro	IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR017926. GATASE_1. [Graphical view]
Gene3D	G3DSA:3.50.30.20. G3DSA:3.50.30.20. 1 hit.
KO	K01956.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
SMART	SM01097. CPSase_sm_chain. 1 hit. [Graphical view]
SUPFAM	SSF52021. CP_synthsmall. 1 hit.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_LEPIN

Accession

Primary (citable) accession number: Q8F6R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	August 10, 2010
Last modified:	January 25, 2012
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program