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Q8F6I1

- SYI_LEPIN

UniProt

Q8F6I1 - SYI_LEPIN

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei557 – 5571Aminoacyl-adenylateUniRule annotation
Binding sitei601 – 6011ATPUniRule annotation
Metal bindingi889 – 8891ZincUniRule annotation
Metal bindingi892 – 8921ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLINT189518:GJBB-1059-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LA_1325
OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Taxonomic identifieri189518 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
ProteomesiUP000001408: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 914914Isoleucine--tRNA ligasePRO_0000098408Add
BLAST

Proteomic databases

PaxDbiQ8F6I1.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi189518.LA1325.

Structurei

3D structure databases

ProteinModelPortaliQ8F6I1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionAdd
BLAST
Motifi598 – 6025"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
InParanoidiQ8F6I1.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8F6I1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSETQKENPY SSTVLLPKTD FPMKADLAKR EPAQIQSWKS GQIFRKMKEQ
60 70 80 90 100
RKGKKEFVLH DGPPYANGNF HLGHSLNKIV KDIIIKSKSL AGFYADMIPG
110 120 130 140 150
WDCHGLPIEV QVLKNLGKKA RETGPEELRR LCRNYAEEFV GKQGEDLSRF
160 170 180 190 200
LCFWEEDRIY KTMSPGFEAK IVEVFGELFQ NGYVYRGKKP VYWSIDLATA
210 220 230 240 250
HAEAEIEYYP HVSPSIYVKF PIVGEQKKFC LIWTTTPWTL PANLGICFNR
260 270 280 290 300
KIEYSFFKTE SGEELILADG LAESVTSTTG VSLNKVKSIS SDELSVLKFQ
310 320 330 340 350
HPFMDRISIS LFGDHVTLDA GTGCVHTAPG HGQDDYKVGL AAGLEPFSPV
360 370 380 390 400
DDYGKYTDEF PLMQGKKVFD ANPEIIQLLK DKGLLLHHSE FEHSYPHSWR
410 420 430 440 450
SKKPLIFRAT PQWFFKMDFN ELREKSLSAI DGVQWIPSWG ITRIRSMVET
460 470 480 490 500
RPDWCLSRQR NWGVPIPAFT CESCGQTHID DVSIQFFTKM VREKGIEIWY
510 520 530 540 550
SEKAADLLPP GTKCGKCGSD SFKKGNDILD VWFDSGVSSF AVLGERKDEP
560 570 580 590 600
PADLYFEGSD QHRGWFQSSL WPSMALRGIP PYKTVLTHGY VLDEKGHPMS
610 620 630 640 650
KSLGNGIDPT ADVIQIYGAD ILRLWVSSLD FRDDIKVGKE SLKIVSEQYR
660 670 680 690 700
KIRNTFRYLL GNLDGHTSEQ NLPFEELEEL DLFYLSKLSQ FAEDVIANYE
710 720 730 740 750
AYQFHQIYQK LILFCTVTLS QDYFDMIRDR MYCDARNSKT RKSSATALQY
760 770 780 790 800
ILETLCILTA PILSFTAEEV WVSNGKKDSV FLQNFPDLKF WKNQNLEEKF
810 820 830 840 850
ESVLQVREVV QKALEIARQE NKLGKSLEAG LEIVSKNGTN LTKILPKETL
860 870 880 890 900
EILFVVSQVH EKNPGLDVLS FYENEKFSVK VLKPVQVECP RCWRHTEDIS
910
KEGDLCGRCK SVVG
Length:914
Mass (Da):104,352
Last modified:March 1, 2003 - v1
Checksum:iF52E6E8801959220
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE010300 Genomic DNA. Translation: AAN48524.1.
RefSeqiNP_711506.1. NC_004342.2.

Genome annotation databases

EnsemblBacteriaiAAN48524; AAN48524; LA_1325.
GeneIDi1150668.
KEGGilil:LA_1325.
PATRICi22383499. VBILepInt91350_1323.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE010300 Genomic DNA. Translation: AAN48524.1 .
RefSeqi NP_711506.1. NC_004342.2.

3D structure databases

ProteinModelPortali Q8F6I1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 189518.LA1325.

Proteomic databases

PaxDbi Q8F6I1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN48524 ; AAN48524 ; LA_1325 .
GeneIDi 1150668.
KEGGi lil:LA_1325.
PATRICi 22383499. VBILepInt91350_1323.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
InParanoidi Q8F6I1.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LINT189518:GJBB-1059-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 56601.

Entry informationi

Entry nameiSYI_LEPIN
AccessioniPrimary (citable) accession number: Q8F6I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3