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Q8F6I1

- SYI_LEPIN

UniProt

Q8F6I1 - SYI_LEPIN

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei557 – 5571Aminoacyl-adenylateUniRule annotation
    Binding sitei601 – 6011ATPUniRule annotation
    Metal bindingi889 – 8891ZincUniRule annotation
    Metal bindingi892 – 8921ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciLINT189518:GJBB-1059-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:LA_1325
    OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
    Taxonomic identifieri189518 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
    ProteomesiUP000001408: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 914914Isoleucine--tRNA ligasePRO_0000098408Add
    BLAST

    Proteomic databases

    PaxDbiQ8F6I1.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi189518.LA1325.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8F6I1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi64 – 7411"HIGH" regionAdd
    BLAST
    Motifi598 – 6025"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8F6I1-1 [UniParc]FASTAAdd to Basket

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    MSETQKENPY SSTVLLPKTD FPMKADLAKR EPAQIQSWKS GQIFRKMKEQ    50
    RKGKKEFVLH DGPPYANGNF HLGHSLNKIV KDIIIKSKSL AGFYADMIPG 100
    WDCHGLPIEV QVLKNLGKKA RETGPEELRR LCRNYAEEFV GKQGEDLSRF 150
    LCFWEEDRIY KTMSPGFEAK IVEVFGELFQ NGYVYRGKKP VYWSIDLATA 200
    HAEAEIEYYP HVSPSIYVKF PIVGEQKKFC LIWTTTPWTL PANLGICFNR 250
    KIEYSFFKTE SGEELILADG LAESVTSTTG VSLNKVKSIS SDELSVLKFQ 300
    HPFMDRISIS LFGDHVTLDA GTGCVHTAPG HGQDDYKVGL AAGLEPFSPV 350
    DDYGKYTDEF PLMQGKKVFD ANPEIIQLLK DKGLLLHHSE FEHSYPHSWR 400
    SKKPLIFRAT PQWFFKMDFN ELREKSLSAI DGVQWIPSWG ITRIRSMVET 450
    RPDWCLSRQR NWGVPIPAFT CESCGQTHID DVSIQFFTKM VREKGIEIWY 500
    SEKAADLLPP GTKCGKCGSD SFKKGNDILD VWFDSGVSSF AVLGERKDEP 550
    PADLYFEGSD QHRGWFQSSL WPSMALRGIP PYKTVLTHGY VLDEKGHPMS 600
    KSLGNGIDPT ADVIQIYGAD ILRLWVSSLD FRDDIKVGKE SLKIVSEQYR 650
    KIRNTFRYLL GNLDGHTSEQ NLPFEELEEL DLFYLSKLSQ FAEDVIANYE 700
    AYQFHQIYQK LILFCTVTLS QDYFDMIRDR MYCDARNSKT RKSSATALQY 750
    ILETLCILTA PILSFTAEEV WVSNGKKDSV FLQNFPDLKF WKNQNLEEKF 800
    ESVLQVREVV QKALEIARQE NKLGKSLEAG LEIVSKNGTN LTKILPKETL 850
    EILFVVSQVH EKNPGLDVLS FYENEKFSVK VLKPVQVECP RCWRHTEDIS 900
    KEGDLCGRCK SVVG 914
    Length:914
    Mass (Da):104,352
    Last modified:March 1, 2003 - v1
    Checksum:iF52E6E8801959220
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010300 Genomic DNA. Translation: AAN48524.1.
    RefSeqiNP_711506.1. NC_004342.2.

    Genome annotation databases

    EnsemblBacteriaiAAN48524; AAN48524; LA_1325.
    GeneIDi1150668.
    KEGGilil:LA_1325.
    PATRICi22383499. VBILepInt91350_1323.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010300 Genomic DNA. Translation: AAN48524.1 .
    RefSeqi NP_711506.1. NC_004342.2.

    3D structure databases

    ProteinModelPortali Q8F6I1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 189518.LA1325.

    Proteomic databases

    PaxDbi Q8F6I1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN48524 ; AAN48524 ; LA_1325 .
    GeneIDi 1150668.
    KEGGi lil:LA_1325.
    PATRICi 22383499. VBILepInt91350_1323.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci LINT189518:GJBB-1059-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 56601.

    Entry informationi

    Entry nameiSYI_LEPIN
    AccessioniPrimary (citable) accession number: Q8F6I1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3