Reviewed, UniProtKB/Swiss-Prot Q8F641 (HPPA_LEPIN)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Ordered Locus Names:	LA_1471

Organism

Leptospira interrogans [Complete proteome] [HAMAP]

Taxonomic identifier

173 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	704 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01129
Cofactor	Magnesium By similarity. Potassium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 1 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium Potassium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 704	704	Pyrophosphate-energized proton pump HAMAP MF_01129		PRO_0000217018
Regions
	Transmembrane	4 – 23	20	Potential
	Transmembrane	64 – 81	18	Potential
	Transmembrane	91 – 113	23	Potential
	Transmembrane	143 – 165	23	Potential
	Transmembrane	175 – 197	23	Potential
	Transmembrane	276 – 298	23	Potential
	Transmembrane	308 – 327	20	Potential
	Transmembrane	348 – 370	23	Potential
	Transmembrane	390 – 412	23	Potential
	Transmembrane	419 – 441	23	Potential
	Transmembrane	481 – 503	23	Potential
	Transmembrane	516 – 538	23	Potential
	Transmembrane	586 – 605	20	Potential
	Transmembrane	612 – 634	23	Potential
	Transmembrane	671 – 693	23	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8F641-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 9369E135382D96BC	FASTA	704	73,106
	10 20 30 40 50 60 MNSVTIIIAM SILAIVTAVV YTLKVTSIKV GTLGGNEKET KKLLEISSAI SEGAMAFLVR 70 80 90 100 110 120 EYKVISLFIA FMAVLIVLLL DNPGSEGFND GIYTAIAFVS GALISCISGF IGMKIATAGN 130 140 150 160 170 180 VRTAEAAKSS MAKAFRVAFD SGAVMGFGLV GLAILGMIVL FLVFTGMYPG VEKHFLMESL 190 200 210 220 230 240 AGFGLGGSAV ALFGRVGGGI YTKAADVGAD LVGKVEKGIP EDDPRNPATI ADNVGDNVGD 250 260 270 280 290 300 VAGMGADLFG SCAEATCAAL VIGATASALS GSVDALLYPL LISAFGIPAS ILTSFLARVK 310 320 330 340 350 360 EDGNVESALK VQLWVSTLLV AGIMYFVTKT FMVDSFEIAG KTITKWDVYI SMVVGLFSGM 370 380 390 400 410 420 FIGIVTEYYT SHSYKPVREV AEASNTGAAT NIIYGLSLGY HSSVIPVILL VITIVTANLL 430 440 450 460 470 480 AGMYGIAIAA LGMISTIAIG LTIDAYGPVS DNAGGIAEMA ELGKEVRDRT DTLDAAGNTT 490 500 510 520 530 540 AAIGKGFAIG SAALTSLALF AAFITRTHTT SLEVLNAEVF GGLMFGAMLP FLFTAMTMKS 550 560 570 580 590 600 VGKAAVDMVE EVRKQFKEIP GIMEGKNKPD YKRCVDISTS AALREMILPG LLVLLTPILV 610 620 630 640 650 660 GYLFGVKTLA GVLAGALVAG VVLAISAANS GGGWDNAKKY IEKKAGGKGS DQHKAAVVGD 670 680 690 700 TVGDPFKDTS GPSINILIKL MAITSLVFAE FFVQQGGLIF KIFH

References

[1]

"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing." Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P. Nature 422:888-893(2003) [PubMed: 12712204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 56601 / Serogroup Icterohaemorrhagiae / Serovar lai.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010300 Genomic DNA. Translation: AAN48670.1.
RefSeq	NP_711652.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1150814.
GenomeReviews	Gene locus LA_1471 in contig AE010300_GR.
KEGG	lil:LA1471.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8F641.
OMA	VFSAMAM.
Enzyme and pathway databases
BioCyc	LINT-130-01:LINT-130-01-002219-MON. LINT189518:LA1471-MON.
BRENDA	3.6.1.1. 258108.
Family and domain databases
HAMAP	MF_01129. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_LEPIN

Accession

Primary (citable) accession number: Q8F641

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)