Reviewed, UniProtKB/Swiss-Prot Q8F5D8 (CHEB2_LEPIN)

Names and origin

Protein names

Recommended name:
    Putative chemotaxis protein-glutamate methylesterase
    EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	LA_1744

Organism

Leptospira interrogans [Complete proteome] [HAMAP]

Taxonomic identifier

173 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in the modulation of the chemotaxis system By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm By similarity HAMAP MF_00099.
Sequence similarities	Contains 1 cheB-type methylesterase domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Putative chemotaxis protein-glutamate methylesterase HAMAP MF_00099		PRO_0000158003
Regions
	Domain	1 – 193	193	CheB-type methylesterase
Sites
	Active site	11	1	By similarity
	Active site	38	1	By similarity
	Active site	131	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8F5D8-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 1F1DB67891BE90BC	FASTA	193	21,292
	10 20 30 40 50 60 MNYEAIVIGV SAGGINAMKT ILPTLPTQFG IPIVIVQHIG ARSDGEWFRI LEKLCNIKIK 70 80 90 100 110 120 EAEEKEEIKS GMVYVAPPNY HLLIEKDKTF SFSIGERVNF SRPSIDVLFE TASEVYEDKL 130 140 150 160 170 180 IGVILTGANS DGAQGLKKIK ENGGLAVVQD PLTAEIALMP RSAIEATSVD YVLSLEKIAE 190 LFIRLDQNNL EQR

References

[1]

"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing." Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P. Nature 422:888-893(2003) [PubMed: 12712204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 56601 / Serogroup Icterohaemorrhagiae / Serovar lai.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010300 Genomic DNA. Translation: AAN48943.1.
RefSeq	NP_711925.1.
3D structure databases
SMR	Q8F5D8. Positions 4-185.
ModBase	Search...
Genome annotation databases
GeneID	1151087.
GenomeReviews	Gene locus LA_1744 in contig AE010300_GR.
KEGG	lil:LA1744.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG705324.
OMA	YSRPSID.
Enzyme and pathway databases
BioCyc	LINT189518:LA1744-MONOMER.
BRENDA	3.1.1.61. 258108.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. Divergent sequence. [Tree]
InterPro	IPR000673. Sig_transdc_resp-reg_Me-estase. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_LEPIN

Accession

Primary (citable) accession number: Q8F5D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	March 1, 2003
Last modified:	January 19, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)