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Q8F4I2 (DDL_LEPIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:LA_2058
OrganismLeptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) [Reference proteome] [HAMAP]
Taxonomic identifier189518 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177835

Regions

Domain135 – 343209ATP-grasp
Nucleotide binding167 – 22256ATP By similarity

Sites

Metal binding2981Magnesium or manganese 1 By similarity
Metal binding3101Magnesium or manganese 1 By similarity
Metal binding3101Magnesium or manganese 2 By similarity
Metal binding3121Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8F4I2 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 9F85D5234E041F58

FASTA35138,591
        10         20         30         40         50         60 
MAKIAVFFGG SSTEHSISIL TGCFICKTLH TMGHSVKPIL LTKDGGWVVP SEYRMSIPFE 

        70         80         90        100        110        120 
VSNSPDLFQE EFQKRYGVSR TNQIFSLDAD IVFLGLHGGQ GEDGTIQGFL EILGIPYTGS 

       130        140        150        160        170        180 
GVLASAIAMD KTRANQIFLQ SGQKVAPFFE IDKLEYLNST DAVITKLETL GFPQFLKPVE 

       190        200        210        220        230        240 
GGSSVSVYKI TNREQLKEKL ALIFESDSKV MSQSFLTGIE VSCGVLERYR DGKFKKIALP 

       250        260        270        280        290        300 
ATEIVPGGEF FDFESKYKQG GSHEITPARI SEQEMKRVQE LAIAAHRSLG CSGYSRTDFI 

       310        320        330        340        350 
IVNGEPHILE TNTLPGMTET SLIPQQAKAA GISMEEVFSD LIEIGLKRSL Y 

« Hide

References

[1]"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing."
Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. expand/collapse author list , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P.
Nature 422:888-893(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 56601.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010300 Genomic DNA. Translation: AAN49258.1.
RefSeqNP_712240.1. NC_004342.2.

3D structure databases

ProteinModelPortalQ8F4I2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING189518.LA2058.

Proteomic databases

PaxDbQ8F4I2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN49258; AAN49258; LA_2058.
GeneID1151402.
KEGGlil:LA_2058.
PATRIC22384969. VBILepInt91350_2055.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAANDTQYR.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycLINT189518:GJBB-1660-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_LEPIN
AccessionPrimary (citable) accession number: Q8F4I2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways