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Protein

Homoserine O-acetyltransferase

Gene

metX

Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the methionine biosynthesis. Catalyzes the transfer of the acetyl group from acetyl-CoA to the hydroxyl group of L-homoserine to yield O-acetyl-L-homoserine.UniRule annotation1 Publication

Catalytic activityi

Acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine.UniRule annotation1 Publication

Kineticsi

  1. KM=1.6 mM for L-homoserine1 Publication
  2. KM=95 mM for acetyl-CoA1 Publication

    Pathwayi: L-methionine biosynthesis via de novo pathway

    This protein is involved in step 1 of the subpathway that synthesizes O-acetyl-L-homoserine from L-homoserine.UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. Homoserine O-acetyltransferase (metX)
    This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes O-acetyl-L-homoserine from L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei153NucleophileUniRule annotation1
    Active sitei311UniRule annotation1
    Active sitei344UniRule annotation1

    GO - Molecular functioni

    • homoserine O-acetyltransferase activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Enzyme and pathway databases

    BRENDAi2.3.1.31. 2986.
    UniPathwayiUPA00051; UER00074.

    Protein family/group databases

    ESTHERilepin-METX. Homoserine_transacetylase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homoserine O-acetyltransferaseUniRule annotation (EC:2.3.1.31UniRule annotation)
    Alternative name(s):
    Homoserine O-trans-acetylaseUniRule annotation
    Short name:
    HTAUniRule annotation
    Short name:
    Homoserine transacetylaseUniRule annotation
    Gene namesi
    Name:metXUniRule annotation
    Ordered Locus Names:LA_2061
    OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
    Taxonomic identifieri189518 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesLeptospiralesLeptospiraceaeLeptospira
    Proteomesi
    • UP000001408 Componenti: Chromosome I

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001557221 – 366Homoserine O-acetyltransferaseAdd BLAST366

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation1 Publication

    Protein-protein interaction databases

    STRINGi189518.LA_2061.

    Structurei

    Secondary structure

    1366
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi13 – 18Combined sources6
    Beta strandi27 – 39Combined sources13
    Beta strandi48 – 52Combined sources5
    Beta strandi62 – 66Combined sources5
    Turni74 – 78Combined sources5
    Beta strandi83 – 86Combined sources4
    Turni87 – 89Combined sources3
    Beta strandi91 – 95Combined sources5
    Beta strandi101 – 106Combined sources6
    Turni112 – 114Combined sources3
    Beta strandi115 – 117Combined sources3
    Helixi119 – 121Combined sources3
    Helixi127 – 140Combined sources14
    Beta strandi144 – 152Combined sources9
    Helixi154 – 165Combined sources12
    Beta strandi169 – 177Combined sources9
    Helixi184 – 198Combined sources15
    Helixi204 – 206Combined sources3
    Beta strandi209 – 211Combined sources3
    Helixi214 – 226Combined sources13
    Helixi231 – 238Combined sources8
    Turni247 – 253Combined sources7
    Helixi256 – 259Combined sources4
    Beta strandi265 – 267Combined sources3
    Helixi271 – 283Combined sources13
    Helixi289 – 296Combined sources8
    Beta strandi301 – 308Combined sources8
    Helixi316 – 328Combined sources13
    Beta strandi333 – 338Combined sources6
    Beta strandi342 – 344Combined sources3
    Helixi345 – 348Combined sources4
    Helixi352 – 363Combined sources12

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2PL5X-ray2.20A1-366[»]
    ProteinModelPortaliQ8F4I0.
    SMRiQ8F4I0.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8F4I0.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini47 – 349AB hydrolase-1Sequence analysisAdd BLAST303

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily. HTA family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105DWV. Bacteria.
    COG2021. LUCA.
    HOGENOMiHOG000246301.
    InParanoidiQ8F4I0.
    KOiK00641.
    OMAiGSVGGMN.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    HAMAPiMF_00296. Homoser_O_acetyltr. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR008220. Homoserine_AcTrfase.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000443. Homoser_Ac_trans. 1 hit.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    TIGRFAMsiTIGR01392. homoserO_Ac_trn. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8F4I0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNETGSIGII ETKYAEFKEL ILNNGSVLSP VVIAYETYGT LSSSKNNAIL
    60 70 80 90 100
    ICHALSGDAH AAGYHSGSDK KPGWWDDYIG PGKSFDTNQY FIICSNVIGG
    110 120 130 140 150
    CKGSSGPLSI HPETSTPYGS RFPFVSIQDM VKAQKLLVES LGIEKLFCVA
    160 170 180 190 200
    GGSMGGMQAL EWSIAYPNSL SNCIVMASTA EHSAMQIAFN EVGRQAILSD
    210 220 230 240 250
    PNWKNGLYDE NSPRKGLALA RMVGHITYLS DDKMREKFGR NPPRGNILST
    260 270 280 290 300
    DFAVGSYLIY QGESFVDRFD ANSYIYVTKA LDHYSLGKGK ELTAALSNAT
    310 320 330 340 350
    CRFLVVSYSS DWLYPPAQSR EIVKSLEAAD KRVFYVELQS GEGHDSFLLK
    360
    NPKQIEILKG FLENPN
    Length:366
    Mass (Da):40,115
    Last modified:March 1, 2003 - v1
    Checksum:i8AADC3E30E30E3F6
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE010300 Genomic DNA. Translation: AAN49260.1.
    RefSeqiNP_712242.1. NC_004342.2.
    WP_001005981.1. NC_004342.2.

    Genome annotation databases

    EnsemblBacteriaiAAN49260; AAN49260; LA_2061.
    GeneIDi1151404.
    KEGGilil:LA_2061.
    PATRICi22384973. VBILepInt91350_2057.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE010300 Genomic DNA. Translation: AAN49260.1.
    RefSeqiNP_712242.1. NC_004342.2.
    WP_001005981.1. NC_004342.2.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2PL5X-ray2.20A1-366[»]
    ProteinModelPortaliQ8F4I0.
    SMRiQ8F4I0.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi189518.LA_2061.

    Protein family/group databases

    ESTHERilepin-METX. Homoserine_transacetylase.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAN49260; AAN49260; LA_2061.
    GeneIDi1151404.
    KEGGilil:LA_2061.
    PATRICi22384973. VBILepInt91350_2057.

    Phylogenomic databases

    eggNOGiENOG4105DWV. Bacteria.
    COG2021. LUCA.
    HOGENOMiHOG000246301.
    InParanoidiQ8F4I0.
    KOiK00641.
    OMAiGSVGGMN.

    Enzyme and pathway databases

    UniPathwayiUPA00051; UER00074.
    BRENDAi2.3.1.31. 2986.

    Miscellaneous databases

    EvolutionaryTraceiQ8F4I0.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    HAMAPiMF_00296. Homoser_O_acetyltr. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR008220. Homoserine_AcTrfase.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000443. Homoser_Ac_trans. 1 hit.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    TIGRFAMsiTIGR01392. homoserO_Ac_trn. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMETX_LEPIN
    AccessioniPrimary (citable) accession number: Q8F4I0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 23, 2003
    Last sequence update: March 1, 2003
    Last modified: November 2, 2016
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.