Q8F4I0 (METX_LEPIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine O-acetyltransferase
EC=2.3.1.31

Alternative name(s):
Homoserine O-trans-acetylase
Short name=HTA
Short name=Homoserine transacetylase

Gene names

Name:	metX
Ordered Locus Names:	LA_2061

Organism

Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) [Reference proteome] [HAMAP]

Taxonomic identifier

189518 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Spirochaetes › Spirochaetia › Spirochaetales › Leptospiraceae › Leptospira › Leptospira interrogans › Leptospira interrogans serovar Lai

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine. HAMAP-Rule MF_00296
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1. HAMAP-Rule MF_00296
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. HTA family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	homoserine O-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 366

366

Homoserine O-acetyltransferase HAMAP-Rule MF_00296

PRO_0000155722

Sites

Active site

153

1

Potential

Active site

344

1

Potential

Secondary structure

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366

Helix Strand Turn

Details...

Beta strand

13 – 18

6

Beta strand

27 – 39

13

Beta strand

48 – 52

5

Beta strand

62 – 66

5

Turn

74 – 78

5

Beta strand

83 – 86

4

Turn

87 – 89

3

Beta strand

91 – 95

5

Beta strand

101 – 106

6

Turn

112 – 114

3

Beta strand

115 – 117

3

Helix

119 – 121

3

Helix

127 – 140

14

Beta strand

144 – 152

9

Helix

154 – 165

12

Beta strand

169 – 177

9

Helix

184 – 198

15

Helix

204 – 206

3

Beta strand

209 – 211

3

Helix

214 – 226

13

Helix

231 – 238

8

Turn

247 – 253

7

Helix

256 – 259

4

Beta strand

265 – 267

3

Helix

271 – 283

13

Helix

289 – 296

8

Beta strand

301 – 308

8

Helix

316 – 328

13

Beta strand

333 – 338

6

Beta strand

342 – 344

3

Helix

345 – 348

4

Helix

352 – 363

12

Sequences

	Sequence		Length	Mass (Da)
	Q8F4I0 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8AADC3E30E30E3F6	FASTA	366	40,115
	10 20 30 40 50 60 MNETGSIGII ETKYAEFKEL ILNNGSVLSP VVIAYETYGT LSSSKNNAIL ICHALSGDAH 70 80 90 100 110 120 AAGYHSGSDK KPGWWDDYIG PGKSFDTNQY FIICSNVIGG CKGSSGPLSI HPETSTPYGS 130 140 150 160 170 180 RFPFVSIQDM VKAQKLLVES LGIEKLFCVA GGSMGGMQAL EWSIAYPNSL SNCIVMASTA 190 200 210 220 230 240 EHSAMQIAFN EVGRQAILSD PNWKNGLYDE NSPRKGLALA RMVGHITYLS DDKMREKFGR 250 260 270 280 290 300 NPPRGNILST DFAVGSYLIY QGESFVDRFD ANSYIYVTKA LDHYSLGKGK ELTAALSNAT 310 320 330 340 350 360 CRFLVVSYSS DWLYPPAQSR EIVKSLEAAD KRVFYVELQS GEGHDSFLLK NPKQIEILKG FLENPN

References

[1]

"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing." Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P. Nature 422:888-893(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 56601.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE010300 Genomic DNA. Translation: AAN49260.1.

RefSeq

NP_712242.1. NC_004342.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PL5	X-ray	2.20	A	1-366	[»]

ProteinModelPortal

Q8F4I0.

SMR

Q8F4I0. Positions 5-366.

ModBase

Search...

Protein-protein interaction databases

STRING

189518.LA2061.

Proteomic databases

PaxDb

Q8F4I0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAN49260; AAN49260; LA_2061.

GeneID

1151404.

KEGG

lil:LA_2061.

PATRIC

22384973. VBILepInt91350_2057.

Phylogenomic databases

eggNOG

COG2021.

HOGENOM

HOG000246301.

KO

K00641.

OMA

VSFTSDW.

ProtClustDB

PRK00175.

Enzyme and pathway databases

BioCyc

LINT189518:GJBB-1662-MONOMER.

UniPathway

UPA00051; UER00074.

Family and domain databases

HAMAP

MF_00296. Homoser_O-acetyltr.

InterPro

IPR000073. AB_hydrolase_1.
IPR008220. Homoserine_AcTrfase.
[Graphical view]

Pfam

PF00561. Abhydrolase_1. 1 hit.
[Graphical view]

PIRSF

PIRSF000443. Homoser_Ac_trans. 1 hit.

TIGRFAMs

TIGR01392. homoserO_Ac_trn. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q8F4I0.

Entry information

Entry name

METX_LEPIN

Accession

Primary (citable) accession number: Q8F4I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program