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Q8F498

- BIOB_LEPIN

UniProt

Q8F498 - BIOB_LEPIN

Protein

Biotin synthase

Gene

bioB

Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 2 (10 Aug 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi107 – 1071Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi139 – 1391Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi199 – 1991Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi269 – 2691Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciLINT189518:GJBB-1737-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:LA_2143
    OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
    Taxonomic identifieri189518 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
    ProteomesiUP000001408: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 351351Biotin synthasePRO_0000381448Add
    BLAST

    Proteomic databases

    PaxDbiQ8F498.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi189518.LA2143.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8F498.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8F498-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKTSEKIFS ETPSIITKEE GLKILNGVIP LTTCLDKAFQ ERNRYFENKV    50
    RIHILDNIKN GYCPEDCGYC AQRKNANSGV QEYPMKSEQE IYEDAVQAKK 100
    NGAYRFCMVT SGTGPNRLTT EKLASTIQRI TDELNMKVCL SAGLLDIEKA 150
    QVLKEAGLDR YNHNLNTSEN HYSEICDTHT YLQRTQTLDS VSKAGIGMCS 200
    GVIVGMGESF QDIVDVAFQL KSFRVISIPV NFFIPVKGHT IKNPSVLTPE 250
    LCVRILCMFR LINPDSEIRI AAGREGHLRS LSATALFAAN SLFSSGYLNV 300
    KGSEILETVA MIRDAGFVPE LSNGEILPEN FGTESFYSEK NFPELYKFKK 350
    F 351
    Length:351
    Mass (Da):39,281
    Last modified:August 10, 2010 - v2
    Checksum:i6031D8D876049768
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010300 Genomic DNA. Translation: AAN49342.2.
    RefSeqiNP_712324.2. NC_004342.2.

    Genome annotation databases

    EnsemblBacteriaiAAN49342; AAN49342; LA_2143.
    GeneIDi1151486.
    KEGGilil:LA_2143.
    PATRICi22385131. VBILepInt91350_2134.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010300 Genomic DNA. Translation: AAN49342.2 .
    RefSeqi NP_712324.2. NC_004342.2.

    3D structure databases

    ProteinModelPortali Q8F498.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 189518.LA2143.

    Proteomic databases

    PaxDbi Q8F498.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN49342 ; AAN49342 ; LA_2143 .
    GeneIDi 1151486.
    KEGGi lil:LA_2143.
    PATRICi 22385131. VBILepInt91350_2134.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci LINT189518:GJBB-1737-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 56601.

    Entry informationi

    Entry nameiBIOB_LEPIN
    AccessioniPrimary (citable) accession number: Q8F498
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: August 10, 2010
    Last modified: October 1, 2014
    This is version 70 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3