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Q8F3V7

- LIPA_LEPIN

UniProt

Q8F3V7 - LIPA_LEPIN

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Protein
Lipoyl synthase
Gene
lipA, LA_2292
Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi58 – 581Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi79 – 791Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi83 – 831Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi86 – 861Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLINT189518:GJBB-1864-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:LA_2292
OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Taxonomic identifieri189518 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
ProteomesiUP000001408: Chromosome I

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Lipoyl synthaseUniRule annotation
PRO_0000102323Add
BLAST

Proteomic databases

PaxDbiQ8F3V7.

Interactioni

Protein-protein interaction databases

STRINGi189518.LA2292.

Structurei

3D structure databases

ProteinModelPortaliQ8F3V7.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8F3V7-1 [UniParc]FASTAAdd to Basket

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MNPLKKKPRT HSLQNAPEKP DWLKVKLAFP DPKNNPVAIV RNSLEEKKLN    50
TVCESASCPN LNHCWSRKTA TYMLGGDICT RRCSYCDVAS GKPFPLDPEE 100
PKRIAESSIA LDLRHVVITS VNRDDLEDGG AAHFAKTVKE IRKGLPDCKI 150
ELLIPDLKVK QEALEIIFEC NPDIFNHNLE TVKRLFPEVA PQKRYERSLD 200
VLKIASARGF LTKSGLILGM GETLEEVKEC MQDLASVGVS LLTLGQYLQP 250
TSTHLPVKEY VVPQVFKDLR IYGKSIGFKG VFSGPLVRSS YHADEQISWN 300
P 301
Length:301
Mass (Da):33,724
Last modified:March 1, 2003 - v1
Checksum:i7E06D76826F1FDB7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE010300 Genomic DNA. Translation: AAN49491.1.
RefSeqiNP_712473.1. NC_004342.2.

Genome annotation databases

EnsemblBacteriaiAAN49491; AAN49491; LA_2292.
GeneIDi1151635.
KEGGilil:LA_2292.
PATRICi22385420. VBILepInt91350_2278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE010300 Genomic DNA. Translation: AAN49491.1 .
RefSeqi NP_712473.1. NC_004342.2.

3D structure databases

ProteinModelPortali Q8F3V7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 189518.LA2292.

Proteomic databases

PaxDbi Q8F3V7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN49491 ; AAN49491 ; LA_2292 .
GeneIDi 1151635.
KEGGi lil:LA_2292.
PATRICi 22385420. VBILepInt91350_2278.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci LINT189518:GJBB-1864-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 56601.

Entry informationi

Entry nameiLIPA_LEPIN
AccessioniPrimary (citable) accession number: Q8F3V7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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