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Q8F3P1

- NRDJ_LEPIN

UniProt

Q8F3P1 - NRDJ_LEPIN

Protein

Vitamin B12-dependent ribonucleotide reductase

Gene

nrdJ

Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (10 Aug 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Cofactori

    5'-deoxyadenosylcobalamine (coenzyme B12).By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei153 – 1531SubstrateBy similarity
    Binding sitei230 – 2301Substrate; via amide nitrogenBy similarity
    Active sitei482 – 4821Proton acceptorBy similarity
    Active sitei484 – 4841Cysteine radical intermediateBy similarity
    Active sitei486 – 4861Proton acceptorBy similarity

    GO - Molecular functioni

    1. cobalamin binding Source: UniProtKB-KW
    2. cobalt ion binding Source: InterPro
    3. nucleotide binding Source: UniProtKB-KW
    4. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    Cobalamin, Cobalt, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciLINT189518:GJBB-1922-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleoside-diphosphate reductase NrdJ
    Gene namesi
    Name:nrdJ
    Ordered Locus Names:LA_2360
    OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
    Taxonomic identifieri189518 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
    ProteomesiUP000001408: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12011201Vitamin B12-dependent ribonucleotide reductasePRO_0000231660Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi199 ↔ 495Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ8F3P1.

    Interactioni

    Protein-protein interaction databases

    STRINGi189518.LA2360.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni198 – 1992Substrate bindingBy similarity
    Regioni482 – 4865Substrate bindingBy similarity
    Regioni683 – 6875Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0209.
    HOGENOMiHOG000021770.
    KOiK00525.

    Family and domain databases

    InterProiIPR013678. RNR_2_N.
    IPR000788. RNR_lg_C.
    IPR013344. RNR_NrdJ/NrdZ.
    IPR024434. TSCPD_dom.
    IPR029072. YebC-like.
    [Graphical view]
    PfamiPF08471. Ribonuc_red_2_N. 1 hit.
    PF02867. Ribonuc_red_lgC. 2 hits.
    PF12637. TSCPD. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    SUPFAMiSSF75625. SSF75625. 1 hit.
    TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8F3P1-1 [UniParc]FASTAAdd to Basket

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    MKMNRHFTVP QNGESSTIQW TKRNSKITNP DGSKVFEAND ILVPEDWSQV     50
    AVDILAQKYF RRKGVPKYLK KVQEDGIPEW LQKSIPDTEK LESLKPEDRF 100
    GGETSALEVF HRLAGCWTYW GYKYKYFSDE ESAKIFYDEI VYMLATQMAA 150
    PNSPQWFNTG LNWAYGIDGK SQGHYYVDPS TGKLVKSTSA YEHPQPHACF 200
    IQSVDDDLVN EGGIMDLWVR EARLFKYGSG TGTNFSNLRG ENEPLSGGGK 250
    SSGLMSFLKI GDRAAGAIKS GGTTRRAAKM VCLDVDHPDI ENFIDWKVTE 300
    EKKVASLVTG SMLNNRHLNA IMSACYEMEG EDRFNPKKNS SLKKTIQDAK 350
    KVLIPDNYIK RVIDLARQGY KEILFEELTT DWQSDAYNTV SGQNSNNSIR 400
    LTNEFMAAVE QDQPWNLYFR TEKEKAKVEG RKAKPSQTLR ARELWEKISY 450
    AAWASADPGT QYHTTINEWH TCPEDGPINA SNPCSEYMFL DNTACNLASA 500
    NLQKFVNLET LNFDVEGFRY LCKLWTIILE ISVTMAQFPS KEIAELSYKF 550
    RTLGLGYANL GSVLMVLGIP YDSQQAMAIT GAISSIMHMT AYATSAEMAK 600
    EQGPFVGYAK NQKHMLRVIR NHRRAAYNAP SGDYEGLTIT PIGINPAFCP 650
    SYMLKAAQED ADLALSLGEK YGFRNAQVTV IAPTGTIGLV MDCDTTGIEP 700
    DFTLVKFKKL AGGGYFKIIN QSVPYGLKKL GYSPSEIEAI VNYCKGHATL 750
    NGAPVINTQA LKEKGFTNEI LEKVEASLPL AFDINFAFNK FNLGENFLTK 800
    NLGISKEIFD SPGFSLLEHL GFTKEDINKA NDYVCGTMTI ENAPFLKEKD 850
    YPVFDCANKC GKYGKRFLSY ESHIRIMAAA QPFISGAISK TINLPEEAVI 900
    EDIKNAYFLS WKMMIKANAL YRDGSKLSQP LNSVLELLNG IEIDDQEEIR 950
    EATISKDPVQ IAEKIVTKYI SHRRKLPSRR AGYTQKAIVG GHKVYLRTGE 1000
    YEDGQIGEIF IDMHKEGAAF RSLMNAFAIS VSLGLQHGVP LEEYVDAFTF 1050
    FKFEPNGIVS GNKHIKMSTS VIDYIFRELA ITYLGRYDLG QVAPEDLRGD 1100
    EIGSKRATAE SNGQEKETLS SMTAVIEPTP KKEVETISYS QMISKEKPSS 1150
    SPSGISLLEE VKLAKIKGYT GDSCSECGSF EMVRNGSCLK CMSCGSTTGC 1200
    S 1201
    Length:1,201
    Mass (Da):133,948
    Last modified:August 10, 2010 - v2
    Checksum:iE62F8C198030B8A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010300 Genomic DNA. Translation: AAN49559.2.
    RefSeqiNP_712541.2. NC_004342.2.
    WP_000783498.1. NC_004342.2.

    Genome annotation databases

    EnsemblBacteriaiAAN49559; AAN49559; LA_2360.
    GeneIDi1151703.
    KEGGilil:LA_2360.
    PATRICi22385550. VBILepInt91350_2342.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010300 Genomic DNA. Translation: AAN49559.2 .
    RefSeqi NP_712541.2. NC_004342.2.
    WP_000783498.1. NC_004342.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 189518.LA2360.

    Proteomic databases

    PaxDbi Q8F3P1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN49559 ; AAN49559 ; LA_2360 .
    GeneIDi 1151703.
    KEGGi lil:LA_2360.
    PATRICi 22385550. VBILepInt91350_2342.

    Phylogenomic databases

    eggNOGi COG0209.
    HOGENOMi HOG000021770.
    KOi K00525.

    Enzyme and pathway databases

    BioCyci LINT189518:GJBB-1922-MONOMER.

    Family and domain databases

    InterProi IPR013678. RNR_2_N.
    IPR000788. RNR_lg_C.
    IPR013344. RNR_NrdJ/NrdZ.
    IPR024434. TSCPD_dom.
    IPR029072. YebC-like.
    [Graphical view ]
    Pfami PF08471. Ribonuc_red_2_N. 1 hit.
    PF02867. Ribonuc_red_lgC. 2 hits.
    PF12637. TSCPD. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    SUPFAMi SSF75625. SSF75625. 1 hit.
    TIGRFAMsi TIGR02504. NrdJ_Z. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 56601.

    Entry informationi

    Entry nameiNRDJ_LEPIN
    AccessioniPrimary (citable) accession number: Q8F3P1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: August 10, 2010
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3