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Q8F3P1

- NRDJ_LEPIN

UniProt

Q8F3P1 - NRDJ_LEPIN

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Protein

Vitamin B12-dependent ribonucleotide reductase

Gene
nrdJ, LA_2360
Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei153 – 1531Substrate By similarity
Binding sitei230 – 2301Substrate; via amide nitrogen By similarity
Active sitei482 – 4821Proton acceptor By similarity
Active sitei484 – 4841Cysteine radical intermediate By similarity
Active sitei486 – 4861Proton acceptor By similarity

GO - Molecular functioni

  1. cobalamin binding Source: UniProtKB-KW
  2. cobalt ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW
  4. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

Cobalamin, Cobalt, Nucleotide-binding

Enzyme and pathway databases

BioCyciLINT189518:GJBB-1922-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene namesi
Name:nrdJ
Ordered Locus Names:LA_2360
OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Taxonomic identifieri189518 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
ProteomesiUP000001408: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12011201Vitamin B12-dependent ribonucleotide reductasePRO_0000231660Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi199 ↔ 495Redox-active By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8F3P1.

Interactioni

Protein-protein interaction databases

STRINGi189518.LA2360.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni198 – 1992Substrate binding By similarity
Regioni482 – 4865Substrate binding By similarity
Regioni683 – 6875Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000021770.
KOiK00525.

Family and domain databases

InterProiIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
IPR029072. YebC-like.
[Graphical view]
PfamiPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 2 hits.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF75625. SSF75625. 1 hit.
TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8F3P1-1 [UniParc]FASTAAdd to Basket

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MKMNRHFTVP QNGESSTIQW TKRNSKITNP DGSKVFEAND ILVPEDWSQV     50
AVDILAQKYF RRKGVPKYLK KVQEDGIPEW LQKSIPDTEK LESLKPEDRF 100
GGETSALEVF HRLAGCWTYW GYKYKYFSDE ESAKIFYDEI VYMLATQMAA 150
PNSPQWFNTG LNWAYGIDGK SQGHYYVDPS TGKLVKSTSA YEHPQPHACF 200
IQSVDDDLVN EGGIMDLWVR EARLFKYGSG TGTNFSNLRG ENEPLSGGGK 250
SSGLMSFLKI GDRAAGAIKS GGTTRRAAKM VCLDVDHPDI ENFIDWKVTE 300
EKKVASLVTG SMLNNRHLNA IMSACYEMEG EDRFNPKKNS SLKKTIQDAK 350
KVLIPDNYIK RVIDLARQGY KEILFEELTT DWQSDAYNTV SGQNSNNSIR 400
LTNEFMAAVE QDQPWNLYFR TEKEKAKVEG RKAKPSQTLR ARELWEKISY 450
AAWASADPGT QYHTTINEWH TCPEDGPINA SNPCSEYMFL DNTACNLASA 500
NLQKFVNLET LNFDVEGFRY LCKLWTIILE ISVTMAQFPS KEIAELSYKF 550
RTLGLGYANL GSVLMVLGIP YDSQQAMAIT GAISSIMHMT AYATSAEMAK 600
EQGPFVGYAK NQKHMLRVIR NHRRAAYNAP SGDYEGLTIT PIGINPAFCP 650
SYMLKAAQED ADLALSLGEK YGFRNAQVTV IAPTGTIGLV MDCDTTGIEP 700
DFTLVKFKKL AGGGYFKIIN QSVPYGLKKL GYSPSEIEAI VNYCKGHATL 750
NGAPVINTQA LKEKGFTNEI LEKVEASLPL AFDINFAFNK FNLGENFLTK 800
NLGISKEIFD SPGFSLLEHL GFTKEDINKA NDYVCGTMTI ENAPFLKEKD 850
YPVFDCANKC GKYGKRFLSY ESHIRIMAAA QPFISGAISK TINLPEEAVI 900
EDIKNAYFLS WKMMIKANAL YRDGSKLSQP LNSVLELLNG IEIDDQEEIR 950
EATISKDPVQ IAEKIVTKYI SHRRKLPSRR AGYTQKAIVG GHKVYLRTGE 1000
YEDGQIGEIF IDMHKEGAAF RSLMNAFAIS VSLGLQHGVP LEEYVDAFTF 1050
FKFEPNGIVS GNKHIKMSTS VIDYIFRELA ITYLGRYDLG QVAPEDLRGD 1100
EIGSKRATAE SNGQEKETLS SMTAVIEPTP KKEVETISYS QMISKEKPSS 1150
SPSGISLLEE VKLAKIKGYT GDSCSECGSF EMVRNGSCLK CMSCGSTTGC 1200
S 1201
Length:1,201
Mass (Da):133,948
Last modified:August 10, 2010 - v2
Checksum:iE62F8C198030B8A6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE010300 Genomic DNA. Translation: AAN49559.2.
RefSeqiNP_712541.2. NC_004342.2.
WP_000783498.1. NC_004342.2.

Genome annotation databases

EnsemblBacteriaiAAN49559; AAN49559; LA_2360.
GeneIDi1151703.
KEGGilil:LA_2360.
PATRICi22385550. VBILepInt91350_2342.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE010300 Genomic DNA. Translation: AAN49559.2 .
RefSeqi NP_712541.2. NC_004342.2.
WP_000783498.1. NC_004342.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 189518.LA2360.

Proteomic databases

PaxDbi Q8F3P1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN49559 ; AAN49559 ; LA_2360 .
GeneIDi 1151703.
KEGGi lil:LA_2360.
PATRICi 22385550. VBILepInt91350_2342.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000021770.
KOi K00525.

Enzyme and pathway databases

BioCyci LINT189518:GJBB-1922-MONOMER.

Family and domain databases

InterProi IPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
IPR029072. YebC-like.
[Graphical view ]
Pfami PF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 2 hits.
PF12637. TSCPD. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF75625. SSF75625. 1 hit.
TIGRFAMsi TIGR02504. NrdJ_Z. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 56601.

Entry informationi

Entry nameiNRDJ_LEPIN
AccessioniPrimary (citable) accession number: Q8F3P1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: August 10, 2010
Last modified: September 3, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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