Q8F3P1 (NRDJ_LEPIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vitamin B12-dependent ribonucleotide reductase
EC=1.17.4.1

Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ

Gene names

Name:	nrdJ
Ordered Locus Names:	LA_2360

Organism

Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) [Reference proteome] [HAMAP]

Taxonomic identifier

189518 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Spirochaetes › Spirochaetia › Spirochaetales › Leptospiraceae › Leptospira › Leptospira interrogans › Leptospira interrogans serovar Lai

Protein attributes

Sequence length	1201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase class-2 family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	Cobalamin Cobalt Nucleotide-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1201	1201	Vitamin B12-dependent ribonucleotide reductase		PRO_0000231660
Sites
	Active site	199	1	By similarity
	Active site	484	1	By similarity
	Active site	495	1	By similarity
	Active site	1194	1	By similarity
	Active site	1200	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8F3P1 [UniParc]. Last modified August 10, 2010. Version 2. Checksum: E62F8C198030B8A6	FASTA	1,201	133,948
	10 20 30 40 50 60 MKMNRHFTVP QNGESSTIQW TKRNSKITNP DGSKVFEAND ILVPEDWSQV AVDILAQKYF 70 80 90 100 110 120 RRKGVPKYLK KVQEDGIPEW LQKSIPDTEK LESLKPEDRF GGETSALEVF HRLAGCWTYW 130 140 150 160 170 180 GYKYKYFSDE ESAKIFYDEI VYMLATQMAA PNSPQWFNTG LNWAYGIDGK SQGHYYVDPS 190 200 210 220 230 240 TGKLVKSTSA YEHPQPHACF IQSVDDDLVN EGGIMDLWVR EARLFKYGSG TGTNFSNLRG 250 260 270 280 290 300 ENEPLSGGGK SSGLMSFLKI GDRAAGAIKS GGTTRRAAKM VCLDVDHPDI ENFIDWKVTE 310 320 330 340 350 360 EKKVASLVTG SMLNNRHLNA IMSACYEMEG EDRFNPKKNS SLKKTIQDAK KVLIPDNYIK 370 380 390 400 410 420 RVIDLARQGY KEILFEELTT DWQSDAYNTV SGQNSNNSIR LTNEFMAAVE QDQPWNLYFR 430 440 450 460 470 480 TEKEKAKVEG RKAKPSQTLR ARELWEKISY AAWASADPGT QYHTTINEWH TCPEDGPINA 490 500 510 520 530 540 SNPCSEYMFL DNTACNLASA NLQKFVNLET LNFDVEGFRY LCKLWTIILE ISVTMAQFPS 550 560 570 580 590 600 KEIAELSYKF RTLGLGYANL GSVLMVLGIP YDSQQAMAIT GAISSIMHMT AYATSAEMAK 610 620 630 640 650 660 EQGPFVGYAK NQKHMLRVIR NHRRAAYNAP SGDYEGLTIT PIGINPAFCP SYMLKAAQED 670 680 690 700 710 720 ADLALSLGEK YGFRNAQVTV IAPTGTIGLV MDCDTTGIEP DFTLVKFKKL AGGGYFKIIN 730 740 750 760 770 780 QSVPYGLKKL GYSPSEIEAI VNYCKGHATL NGAPVINTQA LKEKGFTNEI LEKVEASLPL 790 800 810 820 830 840 AFDINFAFNK FNLGENFLTK NLGISKEIFD SPGFSLLEHL GFTKEDINKA NDYVCGTMTI 850 860 870 880 890 900 ENAPFLKEKD YPVFDCANKC GKYGKRFLSY ESHIRIMAAA QPFISGAISK TINLPEEAVI 910 920 930 940 950 960 EDIKNAYFLS WKMMIKANAL YRDGSKLSQP LNSVLELLNG IEIDDQEEIR EATISKDPVQ 970 980 990 1000 1010 1020 IAEKIVTKYI SHRRKLPSRR AGYTQKAIVG GHKVYLRTGE YEDGQIGEIF IDMHKEGAAF 1030 1040 1050 1060 1070 1080 RSLMNAFAIS VSLGLQHGVP LEEYVDAFTF FKFEPNGIVS GNKHIKMSTS VIDYIFRELA 1090 1100 1110 1120 1130 1140 ITYLGRYDLG QVAPEDLRGD EIGSKRATAE SNGQEKETLS SMTAVIEPTP KKEVETISYS 1150 1160 1170 1180 1190 1200 QMISKEKPSS SPSGISLLEE VKLAKIKGYT GDSCSECGSF EMVRNGSCLK CMSCGSTTGC S

References

[1]

"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing." Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P. Nature 422:888-893(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 56601.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010300 Genomic DNA. Translation: AAN49559.2.
RefSeq	NP_712541.2. NC_004342.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	189518.LA2360.
Proteomic databases
PaxDb	Q8F3P1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN49559; AAN49559; LA_2360.
GeneID	1151703.
KEGG	lil:LA_2360.
PATRIC	22385550. VBILepInt91350_2342.
Phylogenomic databases
eggNOG	COG0209.
HOGENOM	HOG000021770.
KO	K00525.
ProtClustDB	PRK07562.
Enzyme and pathway databases
BioCyc	LINT189518:GJBB-1922-MONOMER.
Family and domain databases
InterPro	IPR013678. RNR_2_N. IPR000788. RNR_lg_C. IPR013344. RNR_NrdJ/NrdZ. IPR024434. TSCPD_dom. [Graphical view]
Pfam	PF08471. Ribonuc_red_2_N. 1 hit. PF02867. Ribonuc_red_lgC. 2 hits. PF12637. TSCPD. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02504. NrdJ_Z. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NRDJ_LEPIN

Accession

Primary (citable) accession number: Q8F3P1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	August 10, 2010
Last modified:	May 1, 2013
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program