Q8F242 (PUR5_LEPIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	LA_2935

Organism

Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) [Reference proteome] [HAMAP]

Taxonomic identifier

189518 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Spirochaetes › Spirochaetia › Spirochaetales › Leptospiraceae › Leptospira › Leptospira interrogans › Leptospira interrogans serovar Lai

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00741.
Sequence similarities	Belongs to the AIR synthase family.
Sequence caution	The sequence AAN50133.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741		PRO_0000148220

Sequences

	Sequence		Length	Mass (Da)
	Q8F242 [UniParc]. Last modified November 28, 2003. Version 2. Checksum: 266AF4D7F5C837EE	FASTA	344	37,761
	10 20 30 40 50 60 MEEKITYKNA GVDTEKGREF IQKIKRNVES THGPRVIGGL GGFAGAYDVS VLKKYKHPIL 70 80 90 100 110 120 LSGTDGVGTK IELARLLKIY NTIGIDLVAM CVNDILVCGG EPLFFLDYIA CGKLDPEKMD 130 140 150 160 170 180 QIVSGIVQGC KMSNASLLGG ETAEHPGTMK EDEFDLAGFV VGAVEKDSMI DGSTIRSGDK 190 200 210 220 230 240 ILGLESSGPH SNGFSLIRKL LLKEGKYLPT DPQQVKFLKD YALKPTRIYV SSILKLLQQV 250 260 270 280 290 300 SVKGMVHITG GGYQENVPRI LPQGTQSKFF KEKIPSGYFF EKIKKDHKIE ELELFATFNM 310 320 330 340 GIGYMVIVSE ENAELAKKIM ESSGEVVHEI GEIVSGNKEE VQFV

References

[1]

"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing." Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P. Nature 422:888-893(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 56601.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010300 Genomic DNA. Translation: AAN50133.2. Different initiation.
RefSeq	NP_713115.2. NC_004342.2.
3D structure databases
ProteinModelPortal	Q8F242.
ModBase	Search...
Protein-protein interaction databases
STRING	189518.LA2935.
Proteomic databases
PaxDb	Q8F242.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN50133; AAN50133; LA_2935.
GeneID	1152277.
KEGG	lil:LA_2935.
PATRIC	22386700. VBILepInt91350_2914.
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HOG000229090.
KO	K01933.
OMA	PRVLPKH.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	LINT189518:GJBB-2372-MONOMER.
UniPathway	UPA00074; UER00129.
Family and domain databases
Gene3D	3.90.650.10. 1 hit.
HAMAP	MF_00741_B. AIRS_B.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_LEPIN

Accession

Primary (citable) accession number: Q8F242

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	May 29, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program