Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8EZY7 (SYL_LEPIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LA_3714
OrganismLeptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) [Reference proteome] [HAMAP]
Taxonomic identifier189518 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152036

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif635 – 6395"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6381ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8EZY7 [UniParc].

Last modified June 20, 2003. Version 2.
Checksum: F108F39E0356E644

FASTA86399,581
        10         20         30         40         50         60 
MQYPFQEVES FWQKFWEEHK SFQTNIRSSK PKFYCLDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LSRFKRMKGF EVLHPMGWDA FGLPAERYAM QTGIHPAITT KDNIDNFRRQ IQMIGLSYDW 

       130        140        150        160        170        180 
SRELSTTDPD YYKFTQWIFI QLYQSWFNPE LKKAESINEL IRRFSNQGSN GLDYRQFNSE 

       190        200        210        220        230        240 
EWKNFSPVEK EKILSDFRLV YQAEIPVNWC EALGTVLANE EVEEWVGKGY EVVRKPMRQY 

       250        260        270        280        290        300 
MMRITAYADR LLEDLELVEW PPSTLEMQKN WIGKSEGLEI TFPFLKPLQS GLEGIRIFTT 

       310        320        330        340        350        360 
RPDTIFGVTY MVVAPEHPIV SEITTPEQKQ KVEEYQKTSS LKSDLDRMEL NKEKTGVFTG 

       370        380        390        400        410        420 
TFVFNPADPS QKIPVWISDY VLYGYGTGAI MAVPAHDQRD FEFARTFGLK IIPVIEGEIS 

       430        440        450        460        470        480 
EAAFDSKTST CINSSSSEIS INGLDYTSAS SKIISWAESK KIGRKKIQFK LRDWLFARQR 

       490        500        510        520        530        540 
YWGEPIPLVH YPSGVTKPIP ESELPLVLPN LEEFKPSGTG ESPLALAKDW LQYKDPSTGE 

       550        560        570        580        590        600 
IGTRETNTMP QWAGSCWYYL RYIDPKNGRF LCDPELEKKW MPVNLYVGGS EHAVLHLLYS 

       610        620        630        640        650        660 
RFWHKFLFDI GAVSTKEPFD KLIHQGLILG EDKRKMSKSL GNVVNPDDVI KEYGADSLRL 

       670        680        690        700        710        720 
FEMFMGPLEM VKPWSTRGVE GVFRFLNRIW RLFHSGSQES FRLDDVEPTP EELKILHKTI 

       730        740        750        760        770        780 
QKVNEDIPNF SFNTAIAQLM IFVNEFTPSD RRPKKVLESF ILLLAPFAPH IAEELWKRSG 

       790        800        810        820        830        840 
KMESLSYEKF PEADPQYLIE SEILIVVQVN GKLRDEFKAP KDVSQSDAIS MAKNLDKIKG 

       850        860 
ILEGKTIRKE IYVPGKLVNL VIG 

« Hide

References

[1]"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing."
Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. expand/collapse author list , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P.
Nature 422:888-893(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 56601.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010300 Genomic DNA. Translation: AAN50912.2.
RefSeqNP_713894.2. NC_004342.2.

3D structure databases

ProteinModelPortalQ8EZY7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING189518.LA3714.

Proteomic databases

PaxDbQ8EZY7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN50912; AAN50912; LA_3714.
GeneID1153056.
KEGGlil:LA_3714.
PATRIC22388261. VBILepInt91350_3690.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycLINT189518:GJBB-2955-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LEPIN
AccessionPrimary (citable) accession number: Q8EZY7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: April 16, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries