Reviewed, UniProtKB/Swiss-Prot Q8EZT7 (PUR2_LEPIN)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	LA_3764

Organism

Leptospira interrogans [Complete proteome] [HAMAP]

Taxonomic identifier

173 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151459
Regions
	Domain	113 – 320	208	ATP-grasp
	Nucleotide binding	139 – 200	62	ATP By similarity
Sites
	Metal binding	290	1	Magnesium or manganese By similarity
	Metal binding	292	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EZT7-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F27E9A51BA9F1DD0	FASTA	426	46,244
	10 20 30 40 50 60 MQVKLKVLLI GSGGRESAIA FYLRKSVLLS ELKVFPGNGG FPDQELLPPD SFQVLDKNSV 70 80 90 100 110 120 QSFLKQNPFD LIVVGPEDPL VAGFADWAAE LNIPVFGPDS FCAQVEGSKD FAKSLMTEAK 130 140 150 160 170 180 IPTAEYKTFS EYSDSLKYLE SKSIPIVIKA DGLAAGKGVT VATSKEMAQT ALKEIFKDKK 190 200 210 220 230 240 FGSSGNQVVI EEFMEGQEAS IFAISDGDSY FLLPAAQDHK RAFDGDQGPN TGGMGAYCPA 250 260 270 280 290 300 PVISESILQK VKEQIFDPMF DLFRKKGHPY RGLLYAGLMI SPNGEPKVVE FNCRFGDPET 310 320 330 340 350 360 QCVLAMLDGD LLELLYRAST GKIKGVQAAV KKGAAVVVVL AAQGYPDFYE KNIPLNLPET 370 380 390 400 410 420 SGQNVHLFHA GTLKKDGKVF SSGGRILGIV AQGADLKSSV DQAYSFLEKI QAPKTFYRKD IGYRAL

References

[1]

"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing." Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P. Nature 422:888-893(2003) [PubMed: 12712204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 56601 / Serogroup Icterohaemorrhagiae / Serovar lai.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010300 Genomic DNA. Translation: AAN50962.1.
RefSeq	NP_713944.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Genome annotation databases
GeneID	1153106.
GenomeReviews	Gene locus LA_3764 in contig AE010300_GR.
KEGG	lil:LA3764.
NMPDR	fig|189518.1.peg.3763.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8EZT7.
OMA	IEYNCRF.
Enzyme and pathway databases
BioCyc	LINT189518:LA3763-MON.
BRENDA	6.3.4.13. 258108.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_LEPIN

Accession

Primary (citable) accession number: Q8EZT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)