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Protein

Argininosuccinate synthase

Gene

argG

Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei91 – 911CitrullineUniRule annotation
Binding sitei96 – 961CitrullineUniRule annotation
Binding sitei121 – 1211ATP; via amide nitrogenUniRule annotation
Binding sitei123 – 1231AspartateUniRule annotation
Binding sitei127 – 1271AspartateUniRule annotation
Binding sitei127 – 1271CitrullineUniRule annotation
Binding sitei128 – 1281AspartateUniRule annotation
Binding sitei131 – 1311CitrullineUniRule annotation
Binding sitei180 – 1801CitrullineUniRule annotation
Binding sitei189 – 1891CitrullineUniRule annotation
Binding sitei265 – 2651CitrullineUniRule annotation
Binding sitei277 – 2771CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 219ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLINT189518:GJBB-3296-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:LA_4165
OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Taxonomic identifieri189518 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesLeptospiralesLeptospiraceaeLeptospira
ProteomesiUP000001408 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Argininosuccinate synthasePRO_0000148607Add
BLAST

Proteomic databases

PaxDbiQ8EYP7.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi189518.LA_4165.

Structurei

3D structure databases

ProteinModelPortaliQ8EYP7.
SMRiQ8EYP7. Positions 9-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
InParanoidiQ8EYP7.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8EYP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQNKPVKKI VLAYSGGLDT SVILTWLKDT YGCEVIAFTA DIGQKEELSG
60 70 80 90 100
LEEKGIKTGA SKVYIQDLRL EFARDFIFPA IQGNAIYEMR YLLGTSLARP
110 120 130 140 150
LIAKAMVEVA EKEGADAFAH GATGKGNDQV RFELGVKSLA PEKTIIAPWR
160 170 180 190 200
IWSFGGRSDL IEYAKSKGIP VPVTAEKPYS MDRNLMHISY EGGILEDPYK
210 220 230 240 250
EPDEKMFLLT TSPEKAPDAP EYLELDFEEG NCVAINGKKM NPLEIMETLN
260 270 280 290 300
TIAGKHGVGR VDIVENRLVG IKSRGVYETP GGTILFLAHR DLESITIDRD
310 320 330 340 350
TQHHKDKLSI EFAELIYNGH WFSSRMKAVR AFITETQRYV SGTVRIKLYK
360 370 380 390 400
GVCSVVGRKS QVSLYNPEMA TFEKEELYNQ KDAEGFINIY GLPAQETARL

RKK
Length:403
Mass (Da):45,061
Last modified:March 1, 2003 - v1
Checksum:i9241C01053C3AD91
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010300 Genomic DNA. Translation: AAN51364.1.
RefSeqiNP_714346.1. NC_004342.2.
WP_000068290.1. NC_004342.2.

Genome annotation databases

EnsemblBacteriaiAAN51364; AAN51364; LA_4165.
GeneIDi1153508.
KEGGilil:LA_4165.
PATRICi22389169. VBILepInt91350_4137.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010300 Genomic DNA. Translation: AAN51364.1.
RefSeqiNP_714346.1. NC_004342.2.
WP_000068290.1. NC_004342.2.

3D structure databases

ProteinModelPortaliQ8EYP7.
SMRiQ8EYP7. Positions 9-401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi189518.LA_4165.

Proteomic databases

PaxDbiQ8EYP7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN51364; AAN51364; LA_4165.
GeneIDi1153508.
KEGGilil:LA_4165.
PATRICi22389169. VBILepInt91350_4137.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
InParanoidiQ8EYP7.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciLINT189518:GJBB-3296-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 56601.

Entry informationi

Entry nameiASSY_LEPIN
AccessioniPrimary (citable) accession number: Q8EYP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: March 1, 2003
Last modified: July 22, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.