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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei256ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLINT189518:G1GL4-3308-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:LA_4190
OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Taxonomic identifieri189518 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesLeptospiralesLeptospiraceaeLeptospira
Proteomesi
  • UP000001408 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001195911 – 521Glutamate--tRNA ligaseAdd BLAST521

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi189518.LA_4190

Structurei

3D structure databases

ProteinModelPortaliQ8EYM3
SMRiQ8EYM3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni320 – 347InsertAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi13 – 23"HIGH" regionUniRule annotationAdd BLAST11
Motifi253 – 257"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
InParanoidiQ8EYM3
KOiK01885
OMAiHYINTLP

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 2 hits
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q8EYM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQSKEVRTR FAPSPSGFLH VGGARTALFN YLYAKSQGGK FILRIEDTDQ
60 70 80 90 100
NRSTEDSFKI ILESLKWLGV NWDEGPEVGG EYGPYIQSQR LNIYKEYTEK
110 120 130 140 150
LLKEKKAYRC FCTQEELEAK KKQSEAMGVP YVYDGLHANM SDEEVQEKLK
160 170 180 190 200
QGIPYSVRFK TPSKTLIIND IIQGKVKFET KLIGDFIIVK SDGFPSYNYA
210 220 230 240 250
VVVDDALMKI THVIRGVGHL SNTPRQILLY EALGYNIPEF AHASEIVGMD
260 270 280 290 300
GKKLSKRAGA TSILAFRDLG YLPETFRNYM ALLGWTSTDG REFLPGDELE
310 320 330 340 350
KIFDVHRCSK SPSTFDVFKK PKGSDEEVVT NFSDLTQIAE AMNPKSKLNW
360 370 380 390 400
LSNRTIRDLN ISKVLENLLP FLMDRKDIPA EVKNVNNPIL TSIVESVRVY
410 420 430 440 450
LDNLTQAPDY VAEFFVTDLK IQSEETIGFL KDGEGPKVVN EFYEILKNSD
460 470 480 490 500
PKTDEDYKNL MSKVGEVTGQ KGKTLYMPIR AATTGKSAGL ELPILFPLLG
510 520
KEKLLQRIEK TSKETGISLS N
Length:521
Mass (Da):58,809
Last modified:March 1, 2003 - v1
Checksum:i995625A4A3D4F98A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010300 Genomic DNA Translation: AAN51388.1
RefSeqiNP_714370.1, NC_004342.2
WP_001076468.1, NC_004342.2

Genome annotation databases

EnsemblBacteriaiAAN51388; AAN51388; LA_4190
GeneIDi1153532
KEGGilil:LA_4190
PATRICifig|189518.3.peg.4162

Similar proteinsi

Entry informationi

Entry nameiSYE_LEPIN
AccessioniPrimary (citable) accession number: Q8EYM3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health