Q8EX97 (ALR_LEPIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	LB_317

Organism

Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) [Reference proteome] [HAMAP]

Taxonomic identifier

189518 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Spirochaetes › Spirochaetia › Spirochaetales › Leptospiraceae › Leptospira › Leptospira interrogans › Leptospira interrogans serovar Lai

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP-Rule MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Alanine racemase HAMAP-Rule MF_01201		PRO_0000114531
Sites
	Active site	48	1	Proton acceptor; specific for D-alanine By similarity
	Active site	281	1	Proton acceptor; specific for L-alanine By similarity
	Binding site	144	1	Substrate By similarity
	Binding site	329	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	48	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EX97 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: B44569674B85B3BF	FASTA	389	43,278
	10 20 30 40 50 60 MQDINSGSSS MKDTYSSWIE ISKRSLSNNL DNFRSILRPN STLTAILKSN AYGHGIEPMT 70 80 90 100 110 120 RLCIEAGISR IGVNSIEEAL LIRNIDSKIP ILIMGEIQNP EKRKDVLSDP NFWIVFSRPE 130 140 150 160 170 180 TARILSSFLP APKLHLKIDT GMGRLGSHGE TLKQTLSELK NVGITLGGIC THFASTEDVL 190 200 210 220 230 240 EHKYSLMQTQ KFEEAIFLAK SFGYNHLIRH ACASASTMLF PNAHFDMVRI GISLYGLWPS 250 260 270 280 290 300 IQTRLSLNLT GNKNFQLNPI LSWKSRIVHI QYHPADSYIG YGSTFQTSYP TKVAIVPVGY 310 320 330 340 350 360 YEGLDRKLSS NGDMLVLGKK ARIIGRICMN MTMLDVTHIP GAEVGSIVTI IGQDGEESIT 370 380 ADDLADRTHT INYEVMTRIS ESIPRIVVD

References

[1]

"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing." Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X., Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X., Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H. , Yin H.-F., Zhang Y., Zhu G.-F., Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y., Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I., Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P. Nature 422:888-893(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 56601.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010301 Genomic DNA. Translation: AAN51876.1.
RefSeq	NP_714861.1. NC_004343.2.
3D structure databases
ProteinModelPortal	Q8EX97.
ModBase	Search...
Protein-protein interaction databases
STRING	189518.LB317.
Proteomic databases
PaxDb	Q8EX97.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN51876; AAN51876; LB_317.
GeneID	1153876.
KEGG	lil:LB_317.
PATRIC	22390170. VBILepInt91350_4636.
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HOG000031444.
KO	K01775.
OMA	RISESIP.
ProtClustDB	CLSK575426.
Enzyme and pathway databases
BioCyc	LINT189518:GJBB-3710-MONOMER.
UniPathway	UPA00042; UER00497.
Family and domain databases
Gene3D	2.40.37.10. 1 hit.
HAMAP	MF_01201. Ala_racemase.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_LEPIN

Accession

Primary (citable) accession number: Q8EX97

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program