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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Mycoplasma penetrans (strain HF-2)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMPEN272633:GJBP-154-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:MYPE1460
OrganismiMycoplasma penetrans (strain HF-2)
Taxonomic identifieri272633 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000002522 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295tRNA(Ile)-lysidine synthasePRO_0000181730Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272633.MYPE1460.

Structurei

3D structure databases

ProteinModelPortaliQ8EWQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107XRM. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000280671.
KOiK04075.
OMAiIVENYCK.
OrthoDBiPOG091H02FP.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8EWQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHKYLIAVS GGPDSMALLN KKRHLVEAVC HVNYHDREDS DNDEKIVRDY
60 70 80 90 100
CKKYNLKLFV FDTHKDDVSK YKDINNLQTW YREIRYDFFE KISQELGIKK
110 120 130 140 150
ILIAHQKNDF LESAYMSLNK NKKNLFLGIR RKSKFRSLIL IRPLLNKTKK
160 170 180 190 200
SLEQYCRSKN IEFVIDYTNF WDRYSRNVVR KMMAEWDKKT FQKFYLKVKW
210 220 230 240 250
FNLKNMFFIK LLDSKFNNWI KQDFDINYFL KIKNNYKESL IYLFLNHIGI
260 270 280 290
KPNENKIEQI IEFINKNKNG SVKKYRLKEN QFIEIKNKKI LYSIC
Length:295
Mass (Da):35,994
Last modified:March 1, 2003 - v1
Checksum:i2F46843ADCBE7F89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000026 Genomic DNA. Translation: BAC43937.1.
RefSeqiWP_011076973.1. NC_004432.1.

Genome annotation databases

EnsemblBacteriaiBAC43937; BAC43937; BAC43937.
KEGGimpe:MYPE1460.
PATRICi20019389. VBIMycPen56664_0150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000026 Genomic DNA. Translation: BAC43937.1.
RefSeqiWP_011076973.1. NC_004432.1.

3D structure databases

ProteinModelPortaliQ8EWQ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272633.MYPE1460.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC43937; BAC43937; BAC43937.
KEGGimpe:MYPE1460.
PATRICi20019389. VBIMycPen56664_0150.

Phylogenomic databases

eggNOGiENOG4107XRM. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000280671.
KOiK04075.
OMAiIVENYCK.
OrthoDBiPOG091H02FP.

Enzyme and pathway databases

BioCyciMPEN272633:GJBP-154-MONOMER.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTILS_MYCPE
AccessioniPrimary (citable) accession number: Q8EWQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.