Q8EWQ5 (ASNA_MYCPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	MYPE1480

Organism

Mycoplasma penetrans (strain HF-2) [Complete proteome] [HAMAP]

Taxonomic identifier

272633 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Aspartate--ammonia ligase HAMAP MF_00555		PRO_0000195883

Sequences

	Sequence		Length	Mass (Da)
	Q8EWQ5 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 701C768BB4194B0A	FASTA	326	37,679
	10 20 30 40 50 60 MYNLSILETQ KAIKFIKDLF QVNLAHALKL HRVTAPLVLE RNKGINDDLN GSENPVTFTS 70 80 90 100 110 120 DGNGISGEIP QSLAKWKRMM LGKYEIPLHE GIYADMNAIR KDESLSSIHS IYVDQWDWEL 130 140 150 160 170 180 HIKKTERNLE TLKVVVKKIY EIIRLCQKEV NKKYEWFAEN LLPEEITFIS SEDLLQRYPN 190 200 210 220 230 240 KTPKERERLI ASKYKAVFII GIGDNLSDGK PHDLRAPDYD DWKLNGDIIV WNETTKSALE 250 260 270 280 290 300 LSSMGIRVDE VSLVEQLDKS NNNSRKELDF HKKLINKEFP YSIGGGIGQS RLCYFLLHKQ 310 320 HIGEVQSSLW PKDILEEAEK NNIKLL

References

[1]

"The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans." Sasaki Y., Ishikawa J., Yamashita A., Oshima K., Kenri T., Furuya K., Yoshino C., Horino A., Shiba T., Sasaki T., Hattori M. Nucleic Acids Res. 30:5293-5300(2002) [PubMed: 12466555] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HF-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000026 Genomic DNA. Translation: BAC43939.1.
RefSeq	NP_757535.1. NC_004432.1.
3D structure databases
ProteinModelPortal	Q8EWQ5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1040788.
GenomeReviews	Gene locus MYPE1480 in contig BA000026_GR.
KEGG	mpe:MYPE1480.
NMPDR	fig|272633.1.peg.149.
PATRIC	20019393. VBIMycPen56664_0152.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
PhylomeDB	Q8EWQ5.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	MPEN272633:MYPE1480-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_MYCPE

Accession

Primary (citable) accession number: Q8EWQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program