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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Mycoplasma penetrans (strain HF-2)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei578 – 5781ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMPEN272633:GJBP-413-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:MYPE3890
OrganismiMycoplasma penetrans (strain HF-2)
Taxonomic identifieri272633 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000002522: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 799799Leucine--tRNA ligasePRO_0000152049Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272633.MYPE3890.

Structurei

3D structure databases

ProteinModelPortaliQ8EW18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 5012"HIGH" regionAdd
BLAST
Motifi575 – 5795"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8EW18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNHNLVEKK WRKIWKDSNL HTFKDNLKKP KFYALDMFPY PSGAGLHMGH
60 70 80 90 100
VKSYTPTDVY ARFKRYQGYS VLHPMGWDAF GLPAEQFALA TKNHPATFTD
110 120 130 140 150
QNINNFKLQI DQLGFWFDWN KEVNTTDPNF YKWTQWIFIK LFENGLAEIK
160 170 180 190 200
DIDVNWCQEL GTVLANEEVL TDEKGNKVSE RGKYPVIKKP MKQWVLKITK
210 220 230 240 250
FADQLIDDLE LTDWTVGLKN IQKKWIGKSI GATVKFKIQN SDSEIEVFTS
260 270 280 290 300
RPDTIFGVSF IGLSSDHELV LKEKTKNKKI EAFLNELSSL KEYERTAINV
310 320 330 340 350
EKKGVLLDIK AIHPITKQLV PVYVCNYVLS NYGNGAIMGV PAGDKRDYDF
360 370 380 390 400
AKLFNLEIKE IIKDHPAPYE EDGIHINSDF LNGLNNEDAI AKIVEYLEKN
410 420 430 440 450
KIGKKQVNYK LKDWLFSRQR YWGEPFPVLF DEKGNIIVEK NLPLLLPETN
460 470 480 490 500
DIKPSGTGES PLANLTEWVN VKIDSKLYRR ETNTMPQWAG SCWYYLAYLL
510 520 530 540 550
KDGDSYLPLD SKQAYEIFKR WLPVDIYIGG QEHAVLHLLY ARFWHKFLHQ
560 570 580 590 600
INVVPNKEPF YKIINQGMIL VNGEKMSKSK GNVVNPSDFV VSHGADALRL
610 620 630 640 650
YMMFMGPITA SLPWEESGID GMYKWVQRVY RLFETKQIDK NFNDENLEKK
660 670 680 690 700
YHQFVKKASE FMENFDFNLV ISEMMIFINE CYKYEKINYD YMLNFCVILS
710 720 730 740 750
CFAPFITEEI NEVFLKNKKF ISDNLWPKYD EKKIVETTIK IPVQINGKIR
760 770 780 790
EVLEINLGAT QKDVVDLAIK NEKIIKWIEN KKIVKEIYIE NKILNLIIK
Length:799
Mass (Da):93,330
Last modified:March 1, 2003 - v1
Checksum:i33B62E3CD73D06FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000026 Genomic DNA. Translation: BAC44178.1.
RefSeqiNP_757774.1. NC_004432.1.
WP_011077214.1. NC_004432.1.

Genome annotation databases

EnsemblBacteriaiBAC44178; BAC44178; BAC44178.
GeneIDi1041130.
KEGGimpe:MYPE3890.
PATRICi20019929. VBIMycPen56664_0402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000026 Genomic DNA. Translation: BAC44178.1.
RefSeqiNP_757774.1. NC_004432.1.
WP_011077214.1. NC_004432.1.

3D structure databases

ProteinModelPortaliQ8EW18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272633.MYPE3890.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC44178; BAC44178; BAC44178.
GeneIDi1041130.
KEGGimpe:MYPE3890.
PATRICi20019929. VBIMycPen56664_0402.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciMPEN272633:GJBP-413-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans."
    Sasaki Y., Ishikawa J., Yamashita A., Oshima K., Kenri T., Furuya K., Yoshino C., Horino A., Shiba T., Sasaki T., Hattori M.
    Nucleic Acids Res. 30:5293-5300(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HF-2.

Entry informationi

Entry nameiSYL_MYCPE
AccessioniPrimary (citable) accession number: Q8EW18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: March 1, 2003
Last modified: March 4, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.