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Q8EVJ8 (DEF_MYCPE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Peptide deformylase
Short name=PDF
EC=3.5.1.88
Alternative name(s):
Polypeptide deformylase
Gene names
Name:def
Ordered Locus Names:MYPE5650
OrganismMycoplasma penetrans (strain HF-2) [Complete proteome] [HAMAP]
Taxonomic identifier272633 [NCBI]
Taxonomic lineageBacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length186 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163

Catalytic activity

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163

Cofactor

Binds 1 Fe2+ ion By similarity. HAMAP MF_00163

Sequence similarities

Belongs to the polypeptide deformylase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandIron
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtranslation

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functioniron ion binding

Inferred from electronic annotation. Source: InterPro

peptide deformylase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 186186Peptide deformylase HAMAP MF_00163
PRO_0000082802

Sites

Active site1581 By similarity
Metal binding1131Iron By similarity
Metal binding1571Iron By similarity
Metal binding1611Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8EVJ8 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: D6E65F8052B19B16

FASTA18621,541
        10         20         30         40         50         60 
MFKKLDPSPK WIVYDNNPVM HKPIEDVVFP LTKEDEHVIS QMLSYVDASY EGEADKYDIR 

        70         80         90        100        110        120 
AGIGIAAIQL GCPKKIIYIH LDDKNGEHKY LMANPKIIKE STSKMYLKNG EGCLSVKKDH 

       130        140        150        160        170        180 
KGLSIRKSIV WVKGIDLFTN KEIEVKATDL LAACFQHEVD HNNNKFYYNR INESDPYYVE 


KNWEEI

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References

[1]"The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans."
Sasaki Y., Ishikawa J., Yamashita A., Oshima K., Kenri T., Furuya K., Yoshino C., Horino A., Shiba T., Sasaki T., Hattori M.
Nucleic Acids Res. 30:5293-5300(2002) [PubMed: 12466555] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HF-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000026 Genomic DNA. Translation: BAC44355.1.
RefSeqNP_757951.1. NC_004432.1.

3D structure databases

ProteinModelPortalQ8EVJ8.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1041435.
GenomeReviewsGene locus MYPE5650 in contig BA000026_GR.
KEGGmpe:MYPE5650.
NMPDRfig|272633.1.peg.565.
PATRIC20020280. VBIMycPen56664_0576.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG665227.
OMALKNGEGC.
PhylomeDBQ8EVJ8.
ProtClustDBPRK00150.

Enzyme and pathway databases

BioCycMPEN272633:MYPE5650-MONOMER.

Family and domain databases

HAMAPMF_00163. Pep_deformylase.
[Tree]
InterProIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
Gene3DG3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KOK01462.
PfamPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFPIRSF004749. Pep_def. 1 hit.
PRINTSPR01576. PDEFORMYLASE.
SUPFAMSSF56420. Fmet_deformylase. 1 hit.
TIGRFAMsTIGR00079. Pept_deformyl. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDEF_MYCPE
AccessionPrimary (citable) accession number: Q8EVJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: March 1, 2003
Last modified: January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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