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Protein

Probable ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Mycoplasma penetrans (strain HF-2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

Cofactori

Mg2+By similarity

Pathway:iglycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. Probable ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogenBy similarity
Metal bindingi103 – 1031Magnesium; catalyticBy similarity
Active sitei127 – 1271Proton acceptorBy similarity
Binding sitei222 – 2221SubstrateBy similarity
Binding sitei249 – 2491Substrate; shared with dimeric partnerBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATPBy similarity
Nucleotide bindingi102 – 1054ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMPEN272633:GJBP-618-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:MYPE5920
OrganismiMycoplasma penetrans (strain HF-2)
Taxonomic identifieri272633 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000002522 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Probable ATP-dependent 6-phosphofructokinasePRO_0000111964Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Protein-protein interaction databases

STRINGi272633.MYPE5920.

Structurei

3D structure databases

ProteinModelPortaliQ8EVH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1273Substrate bindingBy similarity
Regioni169 – 1713Substrate bindingBy similarity
Regioni255 – 2584Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiGRECGYL.
OrthoDBiEOG644ZRM.

Family and domain databases

InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8EVH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIAILTSG GDASTMNKCL STFITYASKY NCEVVFVKNG YKGIYDNEFV
60 70 80 90 100
KPDYTETKSW WSLPGTKIYS SRFPEILDEQ VRKQMVDNLH KNSIDTLVVI
110 120 130 140 150
GGDGSYKGAR LLSKSGIKVI GLPGTIDNDI ASTSYSIGFD TSLNAIVNSI
160 170 180 190 200
KEIKSCMDSH ANVAMIEIMG RHCIDLTVFA GIATEADIII TPESFYTPQQ
210 220 230 240 250
LLAKINEKRK TNSRGIIILY VELLLGTENI PTVEEYIKYI QANSKESVKK
260 270 280 290 300
NILGYLQRGG NPTAMDMIRA SLMTEHALKM ISENQYNKII GVDEFKVVSY
310 320
DLETAINMKN PSRKDLIDKF FN
Length:322
Mass (Da):35,983
Last modified:March 1, 2003 - v1
Checksum:iE88ED024B048CCD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000026 Genomic DNA. Translation: BAC44382.1.
RefSeqiWP_011077414.1. NC_004432.1.

Genome annotation databases

EnsemblBacteriaiBAC44382; BAC44382; BAC44382.
KEGGimpe:MYPE5920.
PATRICi20020336. VBIMycPen56664_0604.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000026 Genomic DNA. Translation: BAC44382.1.
RefSeqiWP_011077414.1. NC_004432.1.

3D structure databases

ProteinModelPortaliQ8EVH2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272633.MYPE5920.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC44382; BAC44382; BAC44382.
KEGGimpe:MYPE5920.
PATRICi20020336. VBIMycPen56664_0604.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiGRECGYL.
OrthoDBiEOG644ZRM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciMPEN272633:GJBP-618-MONOMER.

Family and domain databases

InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans."
    Sasaki Y., Ishikawa J., Yamashita A., Oshima K., Kenri T., Furuya K., Yoshino C., Horino A., Shiba T., Sasaki T., Hattori M.
    Nucleic Acids Res. 30:5293-5300(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HF-2.

Entry informationi

Entry nameiPFKA_MYCPE
AccessioniPrimary (citable) accession number: Q8EVH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2003
Last modified: July 22, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.