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Q8EVH2 (PFKA_MYCPE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:MYPE5920
OrganismMycoplasma penetrans (strain HF-2) [Complete proteome] [HAMAP]
Taxonomic identifier272633 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

Cofactor

Magnesium By similarity.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Subunit structure

Homodimer or homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 322322Probable ATP-dependent 6-phosphofructokinase
PRO_0000111964

Regions

Nucleotide binding72 – 732ATP By similarity
Nucleotide binding102 – 1054ATP By similarity
Region125 – 1273Substrate binding By similarity
Region169 – 1713Substrate binding By similarity
Region255 – 2584Substrate binding By similarity

Sites

Active site1271Proton acceptor By similarity
Metal binding1031Magnesium; catalytic By similarity
Binding site111ATP; via amide nitrogen By similarity
Binding site2221Substrate By similarity
Binding site2491Substrate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8EVH2 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: E88ED024B048CCD3

FASTA32235,983
        10         20         30         40         50         60 
MKKIAILTSG GDASTMNKCL STFITYASKY NCEVVFVKNG YKGIYDNEFV KPDYTETKSW 

        70         80         90        100        110        120 
WSLPGTKIYS SRFPEILDEQ VRKQMVDNLH KNSIDTLVVI GGDGSYKGAR LLSKSGIKVI 

       130        140        150        160        170        180 
GLPGTIDNDI ASTSYSIGFD TSLNAIVNSI KEIKSCMDSH ANVAMIEIMG RHCIDLTVFA 

       190        200        210        220        230        240 
GIATEADIII TPESFYTPQQ LLAKINEKRK TNSRGIIILY VELLLGTENI PTVEEYIKYI 

       250        260        270        280        290        300 
QANSKESVKK NILGYLQRGG NPTAMDMIRA SLMTEHALKM ISENQYNKII GVDEFKVVSY 

       310        320 
DLETAINMKN PSRKDLIDKF FN 

« Hide

References

[1]"The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans."
Sasaki Y., Ishikawa J., Yamashita A., Oshima K., Kenri T., Furuya K., Yoshino C., Horino A., Shiba T., Sasaki T., Hattori M.
Nucleic Acids Res. 30:5293-5300(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HF-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000026 Genomic DNA. Translation: BAC44382.1.
RefSeqNP_757978.1. NC_004432.1.

3D structure databases

ProteinModelPortalQ8EVH2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272633.MYPE5920.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC44382; BAC44382; BAC44382.
GeneID1041468.
KEGGmpe:MYPE5920.
PATRIC20020336. VBIMycPen56664_0604.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248870.
KOK00850.
OMAGFGGRCV.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycMPEN272633:GJBP-618-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

InterProIPR012003. ATP_PFK_prok.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
ProtoNetSearch...

Entry information

Entry namePFKA_MYCPE
AccessionPrimary (citable) accession number: Q8EVH2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways