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Protein

Probable ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Mycoplasma penetrans (strain HF-2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.By similarity
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. Probable ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11ATP; via amide nitrogenBy similarity1
Metal bindingi103Magnesium; catalyticBy similarity1
Active sitei127Proton acceptorBy similarity1
Binding sitei222SubstrateBy similarity1
Binding sitei249Substrate; shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 73ATPBy similarity2
Nucleotide bindingi102 – 105ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMPEN272633:G1G3E-616-MONOMER
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:MYPE5920
OrganismiMycoplasma penetrans (strain HF-2)
Taxonomic identifieri272633 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000002522 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119641 – 322Probable ATP-dependent 6-phosphofructokinaseAdd BLAST322

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272633.MYPE5920

Structurei

3D structure databases

ProteinModelPortaliQ8EVH2
SMRiQ8EVH2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 127Substrate bindingBy similarity3
Regioni169 – 171Substrate bindingBy similarity3
Regioni255 – 258Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107S9E Bacteria
COG0205 LUCA
HOGENOMiHOG000248870
KOiK00850
OMAiALYDLTF
OrthoDBiPOG091H01AC

Family and domain databases

InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit

Sequencei

Sequence statusi: Complete.

Q8EVH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIAILTSG GDASTMNKCL STFITYASKY NCEVVFVKNG YKGIYDNEFV
60 70 80 90 100
KPDYTETKSW WSLPGTKIYS SRFPEILDEQ VRKQMVDNLH KNSIDTLVVI
110 120 130 140 150
GGDGSYKGAR LLSKSGIKVI GLPGTIDNDI ASTSYSIGFD TSLNAIVNSI
160 170 180 190 200
KEIKSCMDSH ANVAMIEIMG RHCIDLTVFA GIATEADIII TPESFYTPQQ
210 220 230 240 250
LLAKINEKRK TNSRGIIILY VELLLGTENI PTVEEYIKYI QANSKESVKK
260 270 280 290 300
NILGYLQRGG NPTAMDMIRA SLMTEHALKM ISENQYNKII GVDEFKVVSY
310 320
DLETAINMKN PSRKDLIDKF FN
Length:322
Mass (Da):35,983
Last modified:March 1, 2003 - v1
Checksum:iE88ED024B048CCD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000026 Genomic DNA Translation: BAC44382.1
RefSeqiWP_011077414.1, NC_004432.1

Genome annotation databases

EnsemblBacteriaiBAC44382; BAC44382; BAC44382
KEGGimpe:MYPE5920

Similar proteinsi

Entry informationi

Entry nameiPFKA_MYCPE
AccessioniPrimary (citable) accession number: Q8EVH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health