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Q8EV81 (TYSY_MYCPE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:MYPE6870
OrganismMycoplasma penetrans (strain HF-2) [Complete proteome] [HAMAP]
Taxonomic identifier272633 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length289 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 289289Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000140986

Sites

Active site1701 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8EV81 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 900CF21403355D46

FASTA28933,890
        10         20         30         40         50         60 
MKEYKDLLKL VLDNGIMQEN RTDTDAIAYF GTQSRYDLTK GFPLLTTKKM AYKAIFHELL 

        70         80         90        100        110        120 
WFLKGETNIK YLVDNNVKIW NEWPYENYKK SPYFKNESID EFILKIKEDD SFAKQFGELG 

       130        140        150        160        170        180 
PVYGRQWRNF NGVDQITNLI EEIKKNKYSR RLIVSVWNPS EIKNMLLPPC HCLFQFFVNS 

       190        200        210        220        230        240 
KNELSCQLYQ RSADLFLGVP FNIASYSLLT YMIAQVTNTT AKEFVHTIGV AHIYNNHIDQ 

       250        260        270        280 
VKEQLTREPL QLPTLVLNKN IKNIFDFKIE DIEIKDYNSH PAIKGKVAV 

« Hide

References

[1]"The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans."
Sasaki Y., Ishikawa J., Yamashita A., Oshima K., Kenri T., Furuya K., Yoshino C., Horino A., Shiba T., Sasaki T., Hattori M.
Nucleic Acids Res. 30:5293-5300(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HF-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000026 Genomic DNA. Translation: BAC44479.1.
RefSeqNP_758075.1. NC_004432.1.

3D structure databases

ProteinModelPortalQ8EV81.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272633.MYPE6870.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC44479; BAC44479; BAC44479.
GeneID1040542.
KEGGmpe:MYPE6870.
PATRIC20020544. VBIMycPen56664_0706.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257899.
KOK00560.
OMANLGPVYG.
OrthoDBEOG6K6V53.
ProtClustDBPRK01827.

Enzyme and pathway databases

BioCycMPEN272633:GJBP-717-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_MYCPE
AccessionPrimary (citable) accession number: Q8EV81
Entry history
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways