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Q8EUN9

- SYI_MYCPE

UniProt

Q8EUN9 - SYI_MYCPE

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma penetrans (strain HF-2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei550 – 5501Aminoacyl-adenylateUniRule annotation
    Binding sitei594 – 5941ATPUniRule annotation
    Metal bindingi871 – 8711ZincUniRule annotation
    Metal bindingi874 – 8741ZincUniRule annotation
    Metal bindingi888 – 8881ZincUniRule annotation
    Metal bindingi891 – 8911ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMPEN272633:GJBP-912-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:MYPE8820
    OrganismiMycoplasma penetrans (strain HF-2)
    Taxonomic identifieri272633 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000002522: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 900900Isoleucine--tRNA ligasePRO_0000098423Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272633.MYPE8820.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8EUN9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi591 – 5955"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8EUN9-1 [UniParc]FASTAAdd to Basket

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    MSDYKKTLSM PNTNFEMKAN LSVKETKIQE NWVLEKIEKK ILAKNKSNTP    50
    FIIADGPPYA NGDLHTGHAL NKILKDIILR FKASVGFYTK YIPGWDTHGL 100
    PIEQEMAKRG LNKNANQTIT EKRKNCKNFA IENVYKQRDQ FRRLGILSEM 150
    DEIYVTCDLD YVIRQIKIFN KMLSKGLIYQ DLKPVYWSWS SQTALADAEI 200
    IYQDVESDSI YVSLEILDKN EFVSKGDKVI IWTTTPWTLP SNLAIAANPK 250
    VTYCRVQVEN SVYVISKNLV EKLAETLEWT NYEILSEFSG KKLENLPYKS 300
    PISDKKCKII VDGYVSDSDG TGLVHNAPGF GHEDYLACKK YNIKPYCPID 350
    NLGKFTGEVN DKELAGLFYK DANPIIIERL AKKKLLLKAS KFTHSAAHDW 400
    RTKKPVIYRA TKQWFVNIDK ISKEIVAALN KVKSIDNTII KKIKEMVLNR 450
    QEWCISRQRI WGVPICIIYD KDYNPILDPK LLNNIVDILN DEGINAWYNE 500
    DAKYFLTDSY NTSKKYIKET DIMDVWFDSG TSYSVLQADK LGYPADLYFE 550
    GKDQFRGWFN SSLITSVAAF KKSPYKELLT HGFVLDENGN KMSKSLGNII 600
    DPLQVCKEYG ADVLRLWVAS VDFSKDVSIS KDIIAQNAEL YRRIRNTLFR 650
    FILGNLNGFN LKKLRGAKYS EADLYVLSCL SNDIKTIKAC YDKYDFKQIV 700
    KIVSKNVADL SSWYFDYIKD PLYCEKEDNE QRIAIQATLY QLLDSYLRIL 750
    APIIPHTCEE AYEFFDKKDK QKSVHLEGFT NFKIDSKYKV DFKKWEEFFD 800
    LKDKVYSEIE KTRNEKVINS KGQAHITIHS KSLPFDEQTL ERYLGVAKVE 850
    FKVKEKDGTT IKVKNSKYAR CERCWNYYPT NLIANELCER CKKVIGNKKI 900
    Length:900
    Mass (Da):104,162
    Last modified:March 1, 2003 - v1
    Checksum:iE7710FE7B619458C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000026 Genomic DNA. Translation: BAC44673.1.
    RefSeqiNP_758269.1. NC_004432.1.

    Genome annotation databases

    EnsemblBacteriaiBAC44673; BAC44673; BAC44673.
    GeneIDi1041417.
    KEGGimpe:MYPE8820.
    PATRICi20020952. VBIMycPen56664_0908.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000026 Genomic DNA. Translation: BAC44673.1 .
    RefSeqi NP_758269.1. NC_004432.1.

    3D structure databases

    ProteinModelPortali Q8EUN9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272633.MYPE8820.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC44673 ; BAC44673 ; BAC44673 .
    GeneIDi 1041417.
    KEGGi mpe:MYPE8820.
    PATRICi 20020952. VBIMycPen56664_0908.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci MPEN272633:GJBP-912-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans."
      Sasaki Y., Ishikawa J., Yamashita A., Oshima K., Kenri T., Furuya K., Yoshino C., Horino A., Shiba T., Sasaki T., Hattori M.
      Nucleic Acids Res. 30:5293-5300(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HF-2.

    Entry informationi

    Entry nameiSYI_MYCPE
    AccessioniPrimary (citable) accession number: Q8EUN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3