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Q8EUN9

- SYI_MYCPE

UniProt

Q8EUN9 - SYI_MYCPE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma penetrans (strain HF-2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei550 – 5501Aminoacyl-adenylateUniRule annotation
Binding sitei594 – 5941ATPUniRule annotation
Metal bindingi871 – 8711ZincUniRule annotation
Metal bindingi874 – 8741ZincUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMPEN272633:GJBP-912-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MYPE8820
OrganismiMycoplasma penetrans (strain HF-2)
Taxonomic identifieri272633 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000002522: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Isoleucine--tRNA ligasePRO_0000098423Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272633.MYPE8820.

Structurei

3D structure databases

ProteinModelPortaliQ8EUN9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8EUN9-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSDYKKTLSM PNTNFEMKAN LSVKETKIQE NWVLEKIEKK ILAKNKSNTP
60 70 80 90 100
FIIADGPPYA NGDLHTGHAL NKILKDIILR FKASVGFYTK YIPGWDTHGL
110 120 130 140 150
PIEQEMAKRG LNKNANQTIT EKRKNCKNFA IENVYKQRDQ FRRLGILSEM
160 170 180 190 200
DEIYVTCDLD YVIRQIKIFN KMLSKGLIYQ DLKPVYWSWS SQTALADAEI
210 220 230 240 250
IYQDVESDSI YVSLEILDKN EFVSKGDKVI IWTTTPWTLP SNLAIAANPK
260 270 280 290 300
VTYCRVQVEN SVYVISKNLV EKLAETLEWT NYEILSEFSG KKLENLPYKS
310 320 330 340 350
PISDKKCKII VDGYVSDSDG TGLVHNAPGF GHEDYLACKK YNIKPYCPID
360 370 380 390 400
NLGKFTGEVN DKELAGLFYK DANPIIIERL AKKKLLLKAS KFTHSAAHDW
410 420 430 440 450
RTKKPVIYRA TKQWFVNIDK ISKEIVAALN KVKSIDNTII KKIKEMVLNR
460 470 480 490 500
QEWCISRQRI WGVPICIIYD KDYNPILDPK LLNNIVDILN DEGINAWYNE
510 520 530 540 550
DAKYFLTDSY NTSKKYIKET DIMDVWFDSG TSYSVLQADK LGYPADLYFE
560 570 580 590 600
GKDQFRGWFN SSLITSVAAF KKSPYKELLT HGFVLDENGN KMSKSLGNII
610 620 630 640 650
DPLQVCKEYG ADVLRLWVAS VDFSKDVSIS KDIIAQNAEL YRRIRNTLFR
660 670 680 690 700
FILGNLNGFN LKKLRGAKYS EADLYVLSCL SNDIKTIKAC YDKYDFKQIV
710 720 730 740 750
KIVSKNVADL SSWYFDYIKD PLYCEKEDNE QRIAIQATLY QLLDSYLRIL
760 770 780 790 800
APIIPHTCEE AYEFFDKKDK QKSVHLEGFT NFKIDSKYKV DFKKWEEFFD
810 820 830 840 850
LKDKVYSEIE KTRNEKVINS KGQAHITIHS KSLPFDEQTL ERYLGVAKVE
860 870 880 890 900
FKVKEKDGTT IKVKNSKYAR CERCWNYYPT NLIANELCER CKKVIGNKKI
Length:900
Mass (Da):104,162
Last modified:March 1, 2003 - v1
Checksum:iE7710FE7B619458C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000026 Genomic DNA. Translation: BAC44673.1.
RefSeqiNP_758269.1. NC_004432.1.
WP_011077702.1. NC_004432.1.

Genome annotation databases

EnsemblBacteriaiBAC44673; BAC44673; BAC44673.
GeneIDi1041417.
KEGGimpe:MYPE8820.
PATRICi20020952. VBIMycPen56664_0908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000026 Genomic DNA. Translation: BAC44673.1 .
RefSeqi NP_758269.1. NC_004432.1.
WP_011077702.1. NC_004432.1.

3D structure databases

ProteinModelPortali Q8EUN9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272633.MYPE8820.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC44673 ; BAC44673 ; BAC44673 .
GeneIDi 1041417.
KEGGi mpe:MYPE8820.
PATRICi 20020952. VBIMycPen56664_0908.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MPEN272633:GJBP-912-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans."
    Sasaki Y., Ishikawa J., Yamashita A., Oshima K., Kenri T., Furuya K., Yoshino C., Horino A., Shiba T., Sasaki T., Hattori M.
    Nucleic Acids Res. 30:5293-5300(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HF-2.

Entry informationi

Entry nameiSYI_MYCPE
AccessioniPrimary (citable) accession number: Q8EUN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3