Q8EU94 (SYE_MYCPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	MYPE10370

Organism

Mycoplasma penetrans (strain HF-2) [Complete proteome] [HAMAP]

Taxonomic identifier

272633 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119607
Regions
	Motif	13 – 23	11	"HIGH" region HAMAP MF_00022_B
	Motif	255 – 259	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	258	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EU94 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 90742E0CB5157E46	FASTA	487	55,636
	10 20 30 40 50 60 MNKNKIIRTR YAPSPTGLFH IGGARTALFN YLFAKKNNGD FIVRIEDTDI DRNVENGIES 70 80 90 100 110 120 QLLNLKWLKI FPDESPLNPG NYGPYIQTER LPIYKEKAEQ LLKEKKAYRC FCTPEQLEKN 130 140 150 160 170 180 RKKALKDGKT PKYNRTCLSL SEDQISEKIK NNIPFSLRFK ITDNTEIKWN DIVRGEMCVP 190 200 210 220 230 240 TSALTDPVIL KSNGIPTYNF AVVIDDNDML ISHIIRGEEH LSNTPYQIAI NEALDINKNI 250 260 270 280 290 300 VFGHLSVIID ETGKKLSKRN MELKQFIEDY KNMGFSPEAI VNFMYLLGLS SSDNKEIFSL 310 320 330 340 350 360 AEAVKNFDIK KVSKSPTTFD FKKMEWISSE HFKMMSDSAF ISFAKPFITI DLGFLKGNEN 370 380 390 400 410 420 DVILLFKNQI AYAKQINDLI DETFFSPETF AAVCEKFPFL KQADTKELIK VFIKQLQELP 430 440 450 460 470 480 NWNISEIATV IDAVKKQTGK SGKELFMPIR VYSSHNSHGP ELAKVIFILG KDKVIKNAKS FLDNKGA

References

[1]

"The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans." Sasaki Y., Ishikawa J., Yamashita A., Oshima K., Kenri T., Furuya K., Yoshino C., Horino A., Shiba T., Sasaki T., Hattori M. Nucleic Acids Res. 30:5293-5300(2002) [PubMed: 12466555] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HF-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000026 Genomic DNA. Translation: BAC44822.1.
RefSeq	NP_758418.1. NC_004432.1.
3D structure databases
ProteinModelPortal	Q8EU94.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1041059.
GenomeReviews	Gene locus MYPE10370 in contig BA000026_GR.
KEGG	mpe:MYPE10370.
NMPDR	fig|272633.1.peg.1032.
PATRIC	20021262. VBIMycPen56664_1061.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	SVKWLLN.
PhylomeDB	Q8EU94.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	MPEN272633:MYPE10370-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_MYCPE

Accession

Primary (citable) accession number: Q8EU94

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program