Q8EU37 (ISPE_OCEIH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	OB0055

Organism

Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831) [Complete proteome] [HAMAP]

Taxonomic identifier

221109 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000189240
Regions
	Nucleotide binding	91 – 101	11	ATP Potential
Sites
	Active site	7	1	By similarity
	Active site	133	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EU37 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 4432A4FF8124FF8E	FASTA	280	30,958
	10 20 30 40 50 60 MEKAPAKINL SLDVLHKRDD GYHDVEMVMT TIDLSDRIEL QQLPKDEIQI ALESRYVPSD 70 80 90 100 110 120 ERNLAYQAAK LFKKTYQIPN GVRIQIEKNI PVSAGLAGGS TDAAAVLRGL NRLFHVNAPL 130 140 150 160 170 180 KDLAKLGSNL GADVAFCVYG KTAIAKGFGD QIEMLPSPPN CWVITARPDI GVSTRTIFER 190 200 210 220 230 240 IELQHLQHPD TKKVLEALQK RDFYQICISL GNALEDITFA LHPEVNRIKN AMLQAGADGA 250 260 270 280 MMSGSGPTVF GLVQHFSKAQ RIYNSMRGFC EDVQLVRILG

References

[1]

"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000028 Genomic DNA. Translation: BAC12011.1.
RefSeq	NP_690976.1. NC_004193.1.
3D structure databases
ProteinModelPortal	Q8EU37.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1015116.
GenomeReviews	Gene locus OB0055 in contig BA000028_GR.
KEGG	oih:OB0055.
NMPDR	fig|221109.1.peg.55.
PATRIC	22790921. VBIOceIhe82024_0055.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	SSTKEMY.
PhylomeDB	Q8EU37.
ProtClustDB	PRK00128.
Enzyme and pathway databases
BioCyc	OIHE221109:OB0055-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_OCEIH

Accession

Primary (citable) accession number: Q8EU37

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program