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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi211 – 218ATP 1By similarity8
Nucleotide bindingi548 – 555ATP 2By similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:OB0093
OrganismiOceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831)
Taxonomic identifieri221109 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
Proteomesi
  • UP000000822 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001911501 – 809Chaperone protein ClpBAdd BLAST809

Proteomic databases

PRIDEiQ8EU05.

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Protein-protein interaction databases

STRINGi221109.OB0093.

Structurei

3D structure databases

ProteinModelPortaliQ8EU05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini420 – 455UVRPROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 144N-terminalBy similarityAdd BLAST144
Regioni163 – 344NBD1By similarityAdd BLAST182
Regioni345 – 488LinkerBy similarityAdd BLAST144
Regioni498 – 716NBD2By similarityAdd BLAST219
Regioni717 – 809C-terminalBy similarityAdd BLAST93

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili395 – 471By similarityAdd BLAST77

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated
Contains 1 UVR domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
HOGENOMiHOG000218210.
KOiK03696.
OMAiDMGIELS.
OrthoDBiPOG091H019M.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
PF02151. UVR. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8EU05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMFGRFTERA QKVLALSQEE AVRLGHNNIG TEHILLGLVR EGEGIAAKAL
60 70 80 90 100
QSLGLEVSKI QEEVEKLIGV GKQPTQSIHY TPRAKKVVEL SQDEARKLGH
110 120 130 140 150
SYVGTEHILL GLIREGEGVA ARVLNNLGVS LNKARQQVLQ LLGSNESQAG
160 170 180 190 200
RQGRSGQQSN ASTPTLDSLA RDLTVSAKEG KIDPVIGRSK EIERVIQVLS
210 220 230 240 250
RRTKNNPVLI GEPGVGKTAV AEGLAQQIID NEVPETLRDK RVMTLDMGTV
260 270 280 290 300
VAGTKYRGEF EDRLKKVMEE IRQAGNIILF IDELHTLIGA GGAEGAIDAS
310 320 330 340 350
NILKPSLARG ELQCIGATTL DEYRKYIEKD AALERRFQPI QVDEPTLEET
360 370 380 390 400
IQILNGLRDR YEAHHRVTIT DEAIEAAASL SDRYITDRFL PDKAIDLIDE
410 420 430 440 450
AGSKVRLRSY TVPPNLKELE QKLDEVRKEK DAAVQSQEFE KAASLRDSEQ
460 470 480 490 500
RFREELETTK NQWKEKQGQT DSEVTMEDIA AVVSTWTGVP VSKLTKDETD
510 520 530 540 550
RLLNMEKILH DRVIGQSEAV NAVAKAIRRA RAGLKDPKRP IGSFIFLGPT
560 570 580 590 600
GVGKTELARA LAEVMFADED AMIRIDMSEY MERHATSRLV GSPPGYVGYD
610 620 630 640 650
EGGQLTEKVR RKPYSVVLLD EVEKAHPEVF NILLQVLEDG RLTDSKGRVV
660 670 680 690 700
DFRNTVIIMT SNVGASELKR NKYVGFALDN EEKDYKDMKS KVIEELKKAF
710 720 730 740 750
RPEFLNRIDE TIVFHSLEKE HMKDIVTLMV QQLQKRLKEQ DLHLSLTDKA
760 770 780 790 800
IEKIANEGFD PEYGARPLRR SIQKNIEDLL SEELLRGAIE KEQQVKIGLN

NKGEFIVLP
Length:809
Mass (Da):90,503
Last modified:March 1, 2003 - v1
Checksum:i4257E790A0B06B7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC12049.1.
RefSeqiWP_011064496.1. NC_004193.1.

Genome annotation databases

EnsemblBacteriaiBAC12049; BAC12049; BAC12049.
KEGGioih:OB0093.
PATRICi22791057. VBIOceIhe82024_0095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC12049.1.
RefSeqiWP_011064496.1. NC_004193.1.

3D structure databases

ProteinModelPortaliQ8EU05.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi221109.OB0093.

Proteomic databases

PRIDEiQ8EU05.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC12049; BAC12049; BAC12049.
KEGGioih:OB0093.
PATRICi22791057. VBIOceIhe82024_0095.

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
HOGENOMiHOG000218210.
KOiK03696.
OMAiDMGIELS.
OrthoDBiPOG091H019M.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
PF02151. UVR. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPB_OCEIH
AccessioniPrimary (citable) accession number: Q8EU05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.