Flavohemoprotein - Oceanobacillus iheyensis

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Reviewed, UniProtKB/Swiss-Prot Q8ETH0 (HMP_OCEIH)

Last modified June 16, 2009. Version 44. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Flavohemoprotein
Alternative name(s):
    Hemoglobin-like protein
    Flavohemoglobin
    Nitric oxide dioxygenase
      Short name=NO oxygenase
      Short name=NOD
    EC=1.14.12.17

Gene names

Name:	hmp
Ordered Locus Names:	OB0291

Organism

Oceanobacillus iheyensis [Complete proteome] [HAMAP]

Taxonomic identifier

182710 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

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Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O₂ and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress By similarity.
Catalytic activity	2 NO + 2 O₂ + NAD(P)H = 2 NO₃^- + NAD(P)⁺. HAMAP MF_01252
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 1 FAD per subunit By similarity.
Domain	Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H. HAMAP MF_01252
Sequence similarities	Belongs to the globin family. Two-domain flavohemoproteins subfamily. In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

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Ontologies

Keywords
Biological process	Detoxification Oxygen transport Transport
Ligand	FAD Flavoprotein Heme Iron Metal-binding NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxygen transport Inferred from electronic annotation. Source: HAMAP response to toxin Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro nitric oxide dioxygenase activity Inferred from electronic annotation. Source: HAMAP oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 406

406

Flavohemoprotein HAMAP MF_01252

PRO_0000052436

Regions

Domain

158 – 267

110

FAD-binding FR-type

Nucleotide binding

212 – 215

FAD By similarity

Nucleotide binding

280 – 285

NADP By similarity

Nucleotide binding

397 – 400

FAD By similarity

Region

5 – 144

140

Globin HAMAP MF_01252

Region

155 – 406

252

Reductase HAMAP MF_01252

Region

271 – 406

136

NAD or NADP-binding HAMAP MF_01252

Sites

Active site

101

Charge relay system By similarity

Active site

143

Charge relay system By similarity

Metal binding

Iron (heme proximal ligand) By similarity

Binding site

196

FAD By similarity

Site

Involved in heme-bound ligand stabilization and O-O bond activation By similarity

Site

Influences the redox potential of the prosthetic heme and FAD groups By similarity

Site

396

Influences the redox potential of the prosthetic heme and FAD groups By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q8ETH0-1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 226846AECD9F4276
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FASTA

406

45,811

        10         20         30         40         50         60 
MSNTTVLLDK KTTEIIKATV PVLKEHGEAI TKHFYKILLE NNPELKNVFN QTNQRKGAQS 

        70         80         90        100        110        120 
KALANTVYAA AANIEKLEEI LPHVKQIAHK HVSLNIKPEQ YPIVGKYLLI AIKEVLGDAA 

       130        140        150        160        170        180 
TDEIIEAWEK AYFVIADIFI SVEKEMYNEK KNQIGGWTGF RDFKVIKKVK ESKEITSFYL 

       190        200        210        220        230        240 
KPDDNLPITT FIPGQYITIK AQIESEAYVH LRQYSLSTAP GKDYYRISVK REASNQPIGV 

       250        260        270        280        290        300 
VSNYLHTSVE VGSVLPISAP AGDFILDERD HRPLVLISGG VGLTPIMSML ESVVEHQPNR 

       310        320        330        340        350        360 
NVVFIHAAKS IDHQAMRKRV SEIAKSKEQV KQYVVYSNPT NRTDGDKQGY IDYEWLKEVI 

       370        380        390        400 
PTKDAAFYLC GPKPFMSAIN NDLQNMNIAQ NDIHMELFGP LEPIAK

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References

[1]

"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
Takami H., Takaki Y., Uchiyama I.
Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

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Cross-references

Sequence databases
	BA000028 Genomic DNA. Translation: BAC12247.1.
RefSeq	NP_691212.1.
3D structure databases
HSSP	HSSP built from PDB template 1VHB based on UniProtKB P04252.
ModBase	Search...
Genome annotation databases
GeneID	1015481.
GenomeReviews	Gene locus OB0291 in contig BA000028_GR.
KEGG	oih:OB0291.
NMPDR	fig\|221109.1.peg.293.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ETH0.
OMA	Q8ETH0. KHRSLGI.
Enzyme and pathway databases
BioCyc	OIHE221109:OB0291-MON.
BRENDA	1.14.12.17. 278212.
Family and domain databases
HAMAP	MF_01252. [Tree]
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR012292. Globin. IPR000971. Globin_subset. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR001221. Phe_hydroxylase. [Graphical view]
Gene3D	G3DSA:1.10.490.10. Globin_related. 1 hit.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00042. Globin. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00371. FPNCR. PR00410. PHEHYDRXLASE.
PROSITE	PS51384. FAD_FR. 1 hit. PS01033. GLOBIN. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

HMP_OCEIH

Accession

Primary (citable) accession number: Q8ETH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents